1978
DOI: 10.1016/0022-2828(78)90381-4
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Adenylate cyclase in cardiac microsomal fractions

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Cited by 14 publications
(3 citation statements)
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“…A sarcolemmal prepartion of guinea pig ventricles was prepared as reported by Drummond et al (1970) with the modifications described (Schrader et al, 1977a). Protein was determined according to the method of Lowry.…”
Section: Analytical Proceduresmentioning
confidence: 99%
“…A sarcolemmal prepartion of guinea pig ventricles was prepared as reported by Drummond et al (1970) with the modifications described (Schrader et al, 1977a). Protein was determined according to the method of Lowry.…”
Section: Analytical Proceduresmentioning
confidence: 99%
“…The enzyme adenylate cyclase has a structure similar to that of the calcium channels, and it is commonly found in the inner layer of the sarcolemma. However, it may also exist in the sarcoplasmic reticulum (SPR) [18][19][20][21] . Usually, adenylate cyclase is activated by the stimulation of β−adrenergic receptors; however, it may also undergo direct action of forskolim @ or be activated by the stimulation of other specific receptors, such as histamine (H 2 ), dopamine (DA 1 ), glucagon and prostacyclin.…”
Section: Signal Transduction -The β β β β β-Adrenergic Pathwaymentioning
confidence: 99%
“…Adenylate cyclase activity was determined by a slight modification of the method of Drummond and Duncan (1970) as previously described (Nij/ar, Au and Chaudhary 1981) with (8-14 C) ATP as substrate. The assay medium contained 20 mM caffeine and 2 mM cyclic AMP to prevent the degradation of radioactive cyclic AMP produced by the reaction.…”
Section: Adenylate Cyclase Assaymentioning
confidence: 99%