Adenylate cyclase in cardiac microsomal fractions

Drummond, George I.; Dunham, Jean

doi:10.1016/0022-2828(78)90381-4

Cited by 14 publications

(3 citation statements)

References 24 publications

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“…A sarcolemmal prepartion of guinea pig ventricles was prepared as reported by Drummond et al (1970) with the modifications described (Schrader et al, 1977a). Protein was determined according to the method of Lowry.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Inhibitory action of adenosine on histamine- and dopamine-stimulated cardiac contractility and adenylate cyclase in guinea pigs.

Baumann¹,

Schrader²,

Gerlach³

1981

Circulation Research

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Infusion of adenosine into the coronary arteries of isolated guinea pig hearts produced a dose-dependent inhibition of cardiac contractile force development elicited by bolus injections of histamine (7.5 X 10(-9) mol) or dopamine (1.5 X 10(-8) mol). Threshold concentration of adenosine was 10(-7) M and maximal inhibition (90%) was obtained at 3 X 10(-5) M. Adenosine in the effective concentration range did not alter Ca2+-induced increases in contractile force. The rise in tissue levels of cAMP induced by equieffective doses of histamine (7.5 X 10(-9) mol) and dopamine (1.5 X 10(-8) mol) was inhibited by adenosine (3.5 X 10(-5) M) by about 60%. In a particular membrane preparation of guinea pig ventricles the adenylate cyclase activity stimulated by the histamine (10(-5) M) and dopamine (10(-4) M) was inhibited in a dose-dependent manner by adenosine. This effect could be reversed by theophylline (5 X 10(-5) M) in a competitive manner. The hormone-insensitive adenylate cyclase of a Lubrol-PX solubilized membrane preparation stimulated by NaF or 5'-guanylylimido-diphosphate (GppNHp) was also inhibited by adenosine (40% and 90% inhibition at 10(-5) M and 10(-4) M, respectively). Adenosine did not influence the Km value of the adenylate cyclase for ATP, but markedly lowered Vmax of the enzyme. From additional studies with purine-substituted (N6-methyl-adenosine, N6-phenylisopropyl-adenosine) and ribose-substituted (2'-deoxy-adenosine and arabino-furanosyl-adenine) adenosine analogues, we conclude that adenosine may inhibit the inotropic responses to hormones as well as the adenylate cyclase activity by specifically interacting with at least two different sites associated with the adenylate cyclase.

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Section: Analytical Proceduresmentioning

confidence: 99%

Inhibitory action of adenosine on histamine- and dopamine-stimulated cardiac contractility and adenylate cyclase in guinea pigs.

Baumann¹,

Schrader²,

Gerlach³

1981

Circulation Research

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“…The enzyme adenylate cyclase has a structure similar to that of the calcium channels, and it is commonly found in the inner layer of the sarcolemma. However, it may also exist in the sarcoplasmic reticulum (SPR) [18][19][20][21] . Usually, adenylate cyclase is activated by the stimulation of β−adrenergic receptors; however, it may also undergo direct action of forskolim @ or be activated by the stimulation of other specific receptors, such as histamine (H 2 ), dopamine (DA 1 ), glucagon and prostacyclin.…”

Section: Signal Transduction -The β β β β β-Adrenergic Pathwaymentioning

confidence: 99%

Beta-adrenergic pathway in healthy and hypertrophied hearts

Barros¹,

Okoshi²,

Cicogna³

1999

Arq. Bras. Cardiol.

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“…Adenylate cyclase activity was determined by a slight modification of the method of Drummond and Duncan (1970) as previously described (Nij/ar, Au and Chaudhary 1981) with (8-14 C) ATP as substrate. The assay medium contained 20 mM caffeine and 2 mM cyclic AMP to prevent the degradation of radioactive cyclic AMP produced by the reaction.…”

Section: Adenylate Cyclase Assaymentioning

confidence: 99%

Endocrine Control of Cytosolic Factors Stimulating Adenylate Cyclase in Rat Lung

Ms²

1988

Horm Metab Res

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Endocrine control of cytoplasmic factors modulating adenylate cyclase activity in rat lung membranes was investigated. Hypophysectomy, adrenalectomy and thyroidectomy showed an adverse effect on the body and organ weights. Lung protein, glycogen and DNA contents were decreased in the endocrine ablated animals which were restored to the normal values on hormone treatment. Phosphodiesterase and phosphorylase activities were increased and decreased in adrenalectomized and thyroidectomized animals, respectively. The activities of these enzymes were restored to normal values on hormone treatment. Adrenalectomy and thyroidectomy affected ATPases differently. Basal adenylate cyclase activity in rat lung membranes was not affected by adrenalectomy and hormone treatment. However, the total enzyme activity was increased by both dexamethasone (DEX) and thyroxine (T4) treatments. The activation of the particulate adenylate cyclase by the cytoplasmic factors was markedly decreased in the lung from hypophysectomized, adrenalectomized and thyroidectomized rats. This decrease in the cytoplasmic activation of adenylate cyclase was restored to or above the control values on hormone treatment. Alteration in the activation of enzyme by cytoplasmic factors did not appear to be due to the change in the responsiveness of the enzyme. Glucocorticoids appeared to have a specific effect on the cytoplasmic factors modulating the enzyme.

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Adenylate cyclase in cardiac microsomal fractions

Cited by 14 publications

References 24 publications

Inhibitory action of adenosine on histamine- and dopamine-stimulated cardiac contractility and adenylate cyclase in guinea pigs.

Inhibitory action of adenosine on histamine- and dopamine-stimulated cardiac contractility and adenylate cyclase in guinea pigs.

Beta-adrenergic pathway in healthy and hypertrophied hearts

Endocrine Control of Cytosolic Factors Stimulating Adenylate Cyclase in Rat Lung

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