Adenylate cyclase from sea urchin eggs is positively and negatively regulated by D-1 and D-2 dopamine receptors

Carginale, Vincenzo; Capasso, Antonio; Madonna, Lucia; Borrelli, Lucia; Parisi, Elio

doi:10.1016/0014-4827(92)90026-5

Cited by 12 publications

(9 citation statements)

References 22 publications

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“…However, the association of Hp-DRD1 with such granules shows remarkable similarity to previous immunohistochemical findings on several subtypes of DA receptors in the human brain (Kumar and Patel, 2007) and multiciliated ependyma in rat, mouse and bovine species (Tomé et al, 2007). The observed DA/DRD1-Gs are far larger than a single GPCR protein (Cherezov et al, 2007), which suggests that each DA/DRD1-G is composed of multiple heteropolymers of components of the DA/DRD1 signaling pathway (Capasso et al, 1988;Carginale et al, 1992;Girault and Greengard, 2004;Arguello and Gogos, 2008).…”

Section: Formation and Sub-cellular Localization Of Da/drd1-gssupporting

confidence: 83%

Development of a dopaminergic system in sea urchin embryos and larvae

Katow

Suyemitsu

Ooka

et al. 2010

Journal of Experimental Biology

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SUMMARYThe mechanisms that regulate the organized swimming movements of sea urchin blastulae are largely unknown. Using immunohistochemistry, we found that dopamine (DA) and the Hemicentrotus pulcherrimus homolog of the dopamine receptor D1 (Hp-DRD1) were strongly co-localized in 1-2m diameter granules (DA/DRD1 granules). Furthermore, these granules were arranged across the entire surface of blastulae as they developed locomotory cilia before hatching, and remained evident until metamorphosis. DA/DRD1 granules were associated with the basal bodies of cilia, and were densely packed in the ciliary band by the eight-arm pluteus stage. The transcription of Hp-DRD1 was detected from the unfertilized egg stage throughout the period of larval development. Treatment with S-(-)-carbidopa, an inhibitor of aromatic-L-amino acid decarboxylase, for 20-24h (i) from soon after insemination until the 20h post-fertilization (20hpf) early gastrula stage and (ii) from the 24hpf prism larva stage until the 48hpf pluteus stage, inhibited the formation of DA granules and decreased the swimming activity of blastulae and larvae in a dose-dependent manner. Exogenous DA rescued these deprivations. The formation of DRD1 granules was not affected. However, in 48hpf plutei, the serotonergic nervous system (5HT-NS) developed normally. Morpholino antisense oligonucleotides directed against Hp-DRD1 inhibited the formation of DRD1 granules and the swimming of larvae, but did not disturb the formation of DA granules. Thus, the formation of DRD1 granules and DA granules occurs chronologically closely but mechanically independently and the swimming of blastulae is regulated by the dopaminergic system. In plutei, the 5HT-NS closely surrounded the ciliary bands, suggesting the functional collaboration with the dopaminergic system in larvae. Supplementary material available online at

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Section: Formation and Sub-cellular Localization Of Da/drd1-gssupporting

confidence: 83%

Development of a dopaminergic system in sea urchin embryos and larvae

Katow

Suyemitsu

Ooka

et al. 2010

Journal of Experimental Biology

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“…The second group is receptors located on the cell surface and plays a role in regulating Ca 2+ levels, transmembrane transport of biogenic amines, and other post-fertilization processes. According to Carginale et al (1992Carginale et al ( , 1995 Lytechinus pictus, embryos from the mesenchyme blastula to the late prism stage, but they observed a significant increase in dopamine level later in development. The authors concluded that the "absence of dopamine in midgastrula embryos seems to be the result of its rapid metabolism to DOPAC and to a lesser extent to its conversion to norepinephrine" by dopamine β-hydroxylase (Anitole-Misleh & Brown, 2004) Studies on the role of dopamine across a variety of invertebrates showed that it affects animal's behavioral response to food stimuli in cnidarians (Hanai & Kitajima, 1984), nematodes (Sawin, Ranganathan, & Horvitz, 2000), insects (Honjo & Furukubo-Tokunaga, 2009;Wright et al, 2010), crustaceans (Van Alstyne, Nelson, Vyvyan, & Cancilla, 2006, molluscs (Kemenes, Hiripi, & Benjamin, 1990;Van Alstyne et al, 2006), and adult sea urchins (Van Alstyne et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Effect of dopamine on early larvae of sea urchins, Mesocentrotus nudus and Strongylocentrotus intermedius

Kalachev

2020

J Exp Zool Pt B

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Larvae of many echinoids are known to be phenotypically plastic and capable of changing the growth rate of their post-oral arms depending on the microalgae concentration in their habitat. As literature data show, developing larvae use chemosensation to detect algae in the environment and "adjust" the rate of growth of their post-oral arms through dopamine signaling. According to our results, dopamine has a significant effect on the post-oral arm growth in early larvae of two sea urchin species, Mesocentrotus nudus and Strongylocentrotus intermedius. The dopamine effect depends on concentration: the higher the dopamine concentration in the water, the shorter the post-oral arms. We suggest that the pattern of response to variation in dopamine concentration, manifested by early larvae of both species, is similar to that observed at different concentrations of microalgae.

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“…This suggests that therapeutically D-amphetamine and methylphenidate may act to enhance 5-HT mediated modulation of sensory activity at the level of the SC, thereby normalising collicular hypersensitivity and reducing symptoms of distractibility. Although metergoline also interacts with dopamine receptors as well as 5-HT receptors [25] and dopamine receptors are present in the SC [26], the agonistic actions of metergoline appear to be largely confined to D2-type dopamine receptors [27], which are scarce in the superficial layers [22]. The involvement of 5-HT is additionally supported by the finding in Dommett et al [24] that the inhibitory action of D-amphetamine and methylphenidate was mimicked by 5-HT itself.…”

Section: Current Pharmacotherapies For Adhdmentioning

confidence: 85%

Enhancing the efficacy of 5-HT uptake inhibitors in the treatment of attention deficit hyperactivity disorder

Riley

Overton

2019

Medical Hypotheses

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Attention Deficit Hyperactivity Disorder (ADHD) is one of the most common childhood behavioural disorders, the frontline treatments for which are drugs with abuse potential. As a consequence, there is an urgent need to develop non addictive drug treatments with equivalent efficacy. Preclinical evidence suggests that selective serotonin uptake inhibitors (SSRIs) are likely to be effective in ADHD, however clinical reports suggest that SSRIs are of limited therapeutic value for the treatment of ADHD. We propose that this disconnect can be explained by the pattern of drug administration in existing clinical trials (administration for short periods of time, or intermittently) leading to inadequate control of the autoregulatory processes which control 5-HT release, most notably at the level of inhibitory 5-HT1A somatodendritic autoreceptors. These autoreceptors reduce the firing rate of 5-HT neurons (limiting release) unless they are desensitised by a long term, frequent pattern of drug administration. As such, we argue that the participants in earlier trials were not administered SSRIs in a manner which realises any potential benefits of targeting 5-HT in the pharmacotherapy of ADHD. In light of this, we hypothesise that there may be under-researched potential to exploit 5-HT transmission therapeutically in ADHD, either through changing the administration regime, or by pharmacological means. Recent pharmacological research has successfully potentiated the effects of SSRIs in acute animal preparations by antagonising inhibitory 5-HT1A autoreceptors prior to the administration of the SSRI fluoxetine. We suggest that combination therapies linking SSRIs and 5-HT1A antagonists are a potential way forward in the development of efficacious nonaddictive pharmacotherapies for ADHD.

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Adenylate cyclase from sea urchin eggs is positively and negatively regulated by D-1 and D-2 dopamine receptors

Cited by 12 publications

References 22 publications

Development of a dopaminergic system in sea urchin embryos and larvae

Development of a dopaminergic system in sea urchin embryos and larvae

Effect of dopamine on early larvae of sea urchins, Mesocentrotus nudus and Strongylocentrotus intermedius

Enhancing the efficacy of 5-HT uptake inhibitors in the treatment of attention deficit hyperactivity disorder

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