Adenylate cyclase activity in Escherichia coli cultured under various conditions

Janeček, J.; Náprstek, J.; Dobrová, Z.; Jirešová, M.; Spížek, J.

doi:10.1111/j.1574-6968.1979.tb03727.x

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Role For Camp In Regulation Of Telmination Of Transcription1

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Cited by 11 publications

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“…Several authors have suggested that adenylate cyclase is a repressible enzyme (176,189). This suggestion is supported by genetic and physiological evidence (26) and by direct measurements of adenylate cyclase activity in broken cell preparations (109,151).…”

Section: Role For Camp In Regulation Of Telmination Of Transcriptionmentioning

confidence: 78%

Cyclic nucleotides in procaryotes.

Botsford¹

1981

Microbiological Reviews

113

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Section: Role For Camp In Regulation Of Telmination Of Transcriptionmentioning

confidence: 78%

Cyclic nucleotides in procaryotes.

Botsford¹

1981

Microbiological Reviews

113

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cAMP and Regulation of Carbohydrate Metabolism

Botsford

1981

Trends in the Biology of Fermentations for Fuels and Chemicals

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Multiple regulation of the activity of adenylate cyclase in Escherichia coli

et al. 1982

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We have studied the correlation between the activities of adenylate cyclase (ATP pyrophosphatelyase-(cyclizing); EC 4.6.1.1) and in vivo rates of synthesis and intracellular concentrations of adenosine 3',5' cyclic monophosphate (cAMP) under various growth conditions in wild-type Escherichia coli and in mutants lacking or overproducing the cAMP receptor protein (CAP). We showed that when wild-type bacteria are grown in the presence of a variety of carbon sources the intracellular concentrations of cAMP are inversely related to the adenylate cyclase activities determined in permeabilized cells, suggesting that the carbon source-dependent modulation of cAMP levels is not directly related to the regulation of adenylate cyclase activity. In mutants lacking functional CAP (crp) the in vivo rates of cAMP synthesis are several hundred-fold higher than in the wild-type parent without a parallel increase of adenylate cyclase activities. In a strain carrying multiple copies of the crp gene and overproducing CAP the activity of adenylate cyclase is severely inhibited, although the in vivo rate of cAMP synthesis is similar to the parental strain. We interpret these results as indicating that CAP controls mainly the activity rather than the synthesis of adenylate cyclase.

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Adenylate cyclase activity in Escherichia coli cultured under various conditions

Cited by 11 publications

References 6 publications

Cyclic nucleotides in procaryotes.

Cyclic nucleotides in procaryotes.

cAMP and Regulation of Carbohydrate Metabolism

Multiple regulation of the activity of adenylate cyclase in Escherichia coli

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