Adenovirus vector-mediated Gli1 siRNA induces growth inhibition and apoptosis in human pancreatic cancer with Smo-dependent or Smo-independent Hh pathway activation in vitro and in vivo

Guo, Jiefang; Gao, Jun; Li, Zhao‐Shen; Yang, Gong; Man, Xiao-hua; Jin, Jing; H, Wu

doi:10.1016/j.canlet.2013.06.010

Cited by 31 publications

(17 citation statements)

References 35 publications

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“…The proteins were then incubated with horseradish peroxidase-conjugated anti-rabbit antibodies (Sigma, St. Louis, MO, USA) for 1 h at 4°C. The proteins were subsequently detected by enhanced chemiluminescence (ECL; Amersham Pharmacia Biotech, Piscataway, NJ, USA) according to the manufacturer’s instructions and quantified by densitometry using UN-SCAN-IT software (Silk Scientific Corp., Orem, UT, USA) (18) with β-actin used as an internal control.…”

Section: Methodsmentioning

confidence: 99%

EBP50 regulates the apoptosis of pancreatic cancer cells by decreasing the expression levels of Bcl-2

Liu²,

et al. 2014

Experimental and Therapeutic Medicine

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Increasing evidence has demonstrated that ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 (EBP50) is involved in the malignant transformation of numerous human cancers. The present study investigated the involvement of EBP50 overexpression in the tumorigenicity of pancreatic cancer (PC). The results revealed that overexpression of EBP50 suppressed cell growth, promoted cell apoptosis and arrested G1-to-S phase progression in two human PC cell lines. Overexpression of EBP50 also suppressed B-cell lymphoma 2 (Bcl-2) expression. Furthermore, nude mouse tumor xenograft models were established by the subcutaneous injection of cell lines stably transfected with an EBP50-expressing plasmid. The in vivo data indicated that overexpression of EBP50 inhibited the growth of the PC tumors and induced cell apoptosis. Thus, the present study demonstrated that EBP50 overexpression induces growth inhibition and apoptosis in PC by decreasing Bcl-2 expression. The results suggest that EBP50 may function as a potential tumor suppressor in vivo and in vitro.

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Section: Methodsmentioning

confidence: 99%

EBP50 regulates the apoptosis of pancreatic cancer cells by decreasing the expression levels of Bcl-2

Liu²,

et al. 2014

Experimental and Therapeutic Medicine

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“…1B) and as well as RMS13 (data not shown). We evaluated whether BCL2 plays a role in the known abilities of GLI1 to promote cell survival (32)(33)(34). We found that BCL2 overexpression was able to reduce the levels of apoptosis induced by the knockdown of GLI1 as detected by changes in annexin V staining (Fig.…”

Section: Gli1 Mediates Tgf␤-induced Gene Expression In Cancermentioning

confidence: 99%

The Transcription Factor GLI1 Interacts with SMAD Proteins to Modulate Transforming Growth Factor β-Induced Gene Expression in a p300/CREB-binding Protein-associated Factor (PCAF)-dependent Manner

Nye

Almada

Fernández‐Barrena

et al. 2014

Journal of Biological Chemistry

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Background:The molecular mechanisms mediating the oncogenic activity of the transcription factor GLI1 remain elusive. Results: GLI1 interacts with SMAD factors and PCAF to regulate TGF␤-induced gene expression. Conclusion:These results define a novel epigenetic mechanism underlying the role of GLI1 as an oncogene. Significance: This study increases our understanding of gene expression regulation in cancer cells and its potential impact in tumor development.

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“…The present study examined the inhibitory potential of GSL on Gli-mediated transcription in human pancreatic cancer PANC-1 cells, which overexpress Gli and are Smo insensitive (2,42). The inhibition of Gli-mediated transcription activity suggests that GSL suppresses downstream of Gli to inhibit cancer cell proliferation or gene expression.…”

Section: Concentration Of Gsl µM -----------------------------------mentioning

confidence: 96%

“…Despite research efforts, there has been limited progress regarding the treatment of this disease; therefore, novel strategies to identify new therapeutic agents are urgently required. The Hedgehog (Hh) signaling pathway is critical for morphogenesis signaling and is inappropriately activated in patients with pancreatic cancer (2,3). In addition, this pathway is associated with the growth and metastasis of various types of tumors, including pancreatic cancer (4,5).…”

Section: Introductionmentioning

confidence: 99%

A sesquiterpene lactone from Siegesbeckia glabrescens suppresses Hedgehog/Gli-mediated transcription in pancreatic cancer cells

Lee

et al. 2016

Oncology Letters

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Abstract. Pancreatic cancer is aggressive and therefore difficult to treat; however, continued efforts have been made with the aim of developing an effective therapy against the disease. The Hedgehog (Hh) signaling pathway is reportedly involved in the proliferation and survival of pancreatic cancer cells. The transcription factor glioma-associated oncogene (Gli) is a key component of the Hh signaling pathway and the primary effector of pancreatic cancer development. Inhibiting Gli is a proven therapeutic strategy for this disease. The present study examined the regulation of Gli and the expression of its target genes to identify an inhibitor of the Sonic Hh (Shh) pathway. A germacranolide sesquiterpene lactone (GSL) was isolated from Siegesbeckia glabrescens as an inhibitor of Gli-mediated transcription. The results demonstrated that GSL inhibited Shh-induced osteoblast differentiation and Gli homolog 1 (Gli1)-mediated transcriptional activity in mesenchymal C3H10T1/2 stem cells. Furthermore, GSL suppressed Gli-mediated transcriptional activity in human pancreatic cancer PANC-1 and AsPC-1 cells, which resulted in reduced cancer cell proliferation and downregulated expression of the Gli-target genes, Gli1 and cyclin D1. A sesquiterpene lactone from S. glabrescens may therefore serve as a candidate for the treatment of Hh/Gli-dependent pancreatic cancer.

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Adenovirus vector-mediated Gli1 siRNA induces growth inhibition and apoptosis in human pancreatic cancer with Smo-dependent or Smo-independent Hh pathway activation in vitro and in vivo

Cited by 31 publications

References 35 publications

EBP50 regulates the apoptosis of pancreatic cancer cells by decreasing the expression levels of Bcl-2

EBP50 regulates the apoptosis of pancreatic cancer cells by decreasing the expression levels of Bcl-2

The Transcription Factor GLI1 Interacts with SMAD Proteins to Modulate Transforming Growth Factor β-Induced Gene Expression in a p300/CREB-binding Protein-associated Factor (PCAF)-dependent Manner

A sesquiterpene lactone from Siegesbeckia glabrescens suppresses Hedgehog/Gli-mediated transcription in pancreatic cancer cells

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