Adenovirus type 40 and 41 growth in vitro: host range diversity reflected by differences in patterns of DNA replication

Tiemessen, Caroline T.; Kidd, Alistair H.

doi:10.1128/jvi.68.2.1239-1244.1994

Cited by 31 publications

(18 citation statements)

References 30 publications

(33 reference statements)

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“…The fastidiousness of enteric adenoviruses has made it difficult to select an optimum cell line for culturing these viruses (Brown et al 1992;Tiemessen and Kidd 1994). As a result, there are few cells known to be capable of enteric adenoviruses.…”

Section: Discussionmentioning

confidence: 99%

The simultaneous detection of both enteroviruses and adenoviruses in environmental water samples including tap water with an integrated cell culture-multiplex-nested PCR procedure

Lee

et al. 2005

J Appl Microbiol

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Aims:To evaluate an integrated cell culture (ICC)-multiplex-nested PCR using the buffalo green monkey kidney (BGMK) cells for the simultaneous detection of both enteroviruses and adenoviruses in surface water and tap water samples and optimize the procedure for more sensitive detection of virus showing no apparent cytopathic effect (CPE). Methods and Results: A total of 69 surface water and 50 tap water samples were analysed by the ICC-multiplexnested PCR. All the PCRs were performed five times with a cell lysate from each flask after at least 2 weeks incubation. Forty-six surface water samples (66AE7%) and 23 tap water samples (46AE0%) exhibited CPE by the cell culture method. By using an ICC-multiplex-nested PCR, 53 surface water samples (76AE8%) and 29 tap water samples (58AE0%) were determined as containing infectious enteric viral particles. Conclusions: An ICC-PCR method with a long incubation time using BGMK cells enables the simultaneous detection of enteroviruses and adenoviruses from environmental water samples, including tap water, even with low numbers of viruses. Significance and Impact of the Study: A method capable of detecting small numbers of viral particles is necessary.

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Section: Discussionmentioning

confidence: 99%

The simultaneous detection of both enteroviruses and adenoviruses in environmental water samples including tap water with an integrated cell culture-multiplex-nested PCR procedure

Lee

et al. 2005

J Appl Microbiol

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“…Infection and replication of prototype HAdV strains in cell lines. Reports of enteric HAdV-F replication in transformed cells are inconsistent (4,7,8,26,27). We therefore compared the replications of the prototype strains HAdV-41p (HAdV-F), HAdV-5p (HAdV-C), and HAdV-16p (HAdV-B) in two standard cell lines, A549 cells (lung carcinoma) and 293 (embryonic kidney), to determine whether the culture defect for HAdV-F was at the level of initial infection, replication, or both.…”

Section: Resultsmentioning

confidence: 99%

Adenovirus Infection of Human Enteroids Reveals Interferon Sensitivity and Preferential Infection of Goblet Cells

Holly

Smith

2018

J Virol

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Human adenoviruses (HAdV) are significant human pathogens. Although only a subset of HAdV serotypes commonly cause gastroenteritis in humans, most HAdV species replicate in the gastrointestinal tract. Knowledge of the complex interaction between HAdVs and the human intestinal epithelium has been limited by the lack of a suitable cell culture system containing relevant cell types. Recently, this need has been met by the stable and prolonged cultivation of primary intestinal epithelial cells as enteroids. Human enteroids have been used to reveal novel and interesting aspects of rotavirus, norovirus, and enterovirus replication, prompting us to explore their suitability for HAdV culture. We found that both prototype strains and clinical isolates of enteric and non-enteric HAdVs productively replicate in human enteroids. HAdV-5p, a respiratory pathogen, and HAdV-41p, an enteric pathogen, are both sensitive to type I and III interferons in human enteroid monolayers but not A549 cells. Interestingly, HAdV-5p, but not HAdV-41p, preferentially infected goblet cells. And, HAdV-5p but not HAdV-41p was potently neutralized by the enteric human alpha-defensin HD5. These studies highlight new facets of HAdV biology that are uniquely revealed by primary intestinal epithelial cell culture. Enteric adenoviruses are a significant cause of childhood gastroenteritis worldwide, yet our understanding of their unique biology is limited. Here we report robust replication of both prototype and clinical isolates of enteric and respiratory human adenoviruses in enteroids, a primary intestinal cell culture system. Recent studies have shown that other fastidious enteric viruses replicate in human enteroids. Therefore, human enteroids may provide a unified platform for culturing enteric viruses, potentially enabling isolation of a greater diversity of viruses from patients. Moreover, both the ability of interferon to restrict respiratory and enteric adenoviruses and a surprising preference of a respiratory serotype for goblet cells demonstrates the power of this culture system to uncover aspects of adenovirus biology that were previously unattainable with standard cell lines.

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“…However, some adenoviruses, such as Ad40 and Ad41, do not plaque efficiently, so a most‐probable‐number procedure may be used (Meng & Gerba, 1996). There is no standard cell line used for the detection of adenoviruses; cells used include A549, Caco‐2, Chang, HEK, G293, HeLa, HEp2, and PLC/PRF/5 cell lines (Rigotto et al, 2005; Ko et al, 2003; Thompson et al, 2003; Meng & Gerba, 1996; Tiemessen & Kidd, 1994; Brown et al, 1992). Each of these cell lines may be more selective for some adenoviruses serotypes than for others, making it difficult to compare results among studies.…”

Section: Methodsmentioning

confidence: 99%

Effect of adenovirus resistance on UV disinfection requirements: A report on the state of adenovirus science

Yates¹,

Malley²,

Rochelle³

et al. 2006

Journal AWWA

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The dosage of ultraviolet (UV) light required to inactivate adenoviruses serves as the basis for virus inactivation requirements in the Long Term 2 Enhanced Surface Water Treatment Rule.The rule increases the required UV dose from the standard practice of 40 mJ/cm 2 to 186 mJ/cm 2 for 4-log inactivation. Ensuring this delivered dose in the UV reactor requires accounting for uncertainties in the reactor validation testing, which results in an applied UV dose of 200-300 mJ/cm 2 for 4-log virus inactivation credit for a given UV reactor. Concerned about the potential effect of this action on drinking water treatment, a group of experts met to assess the state of the science with respect to adenoviruses in drinking water. This working group reviewed the current science on adenoviruses and identified the effects-positive and negative-on public health protection arising from the elevation of UV design target doses.

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Adenovirus type 40 and 41 growth in vitro: host range diversity reflected by differences in patterns of DNA replication

Cited by 31 publications

References 30 publications

The simultaneous detection of both enteroviruses and adenoviruses in environmental water samples including tap water with an integrated cell culture-multiplex-nested PCR procedure

The simultaneous detection of both enteroviruses and adenoviruses in environmental water samples including tap water with an integrated cell culture-multiplex-nested PCR procedure

Adenovirus Infection of Human Enteroids Reveals Interferon Sensitivity and Preferential Infection of Goblet Cells

Effect of adenovirus resistance on UV disinfection requirements: A report on the state of adenovirus science

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