Adenovirus-mediated ING4/PTEN double tumor suppressor gene co-transfer modified by RGD enhances antitumor activity in human nasopharyngeal carcinoma cells

Wang, Yihong; Yang, Jicheng; Sheng, Wei; Xie, Yufeng; Liu, Jisheng

doi:10.3892/ijo.2015.2822

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…In lung cancer tissues, decreased ING4 expression is present in ~50% of cases and is associated with lymph node metastasis (29). Together with the results of other surveys, ING4 is a promising target in gene-radiotherapy (30)(31)(32).…”

Section: Discussionmentioning

confidence: 95%

Lentiviral vector with a radiation‑inducible promoter, carrying the ING4 gene, mediates radiosensitization controlled by radiotherapy in cervical cancer cells

Guo

Wang

et al. 2020

Oncol Lett

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The presence of hypoxia in solid tumors is considered one of the major factors that contribute to radiation resistance. The aim of the present study was to establish a therapeutic system, which can be controlled by radiation itself, to enhance radiosensitivity. For this purpose, a lentiviral gene therapy vector containing the human inhibitor of growth 4 (ING4) and its upstream promoter, human early growth response factor-1 (EGR1), which possesses the radiation-inducible characteristics to activate the transcription of its downstream genes, was constructed. Downstream fluorescence proteins were investigated to ensure that the EGR1 promoter was induced by irradiation. Furthermore, ING4 open reading frame (ORF) expression was detected by western blotting. The cell cycle was analyzed by fluorescence-activated cell sorting analysis 48 h after the cells were exposed to X-rays ranging between 0 and 8 Gy. In cells stably and transiently transfected with reporter plasmids, the EGR1-driver gene was sensitive to ionizing irradiation. Furthermore, irradiation-induced ING4 gene expression was observed. The enhanced ING4 expression increased the number of cells in the G 2 /M phase and decreased the proportion of cells in the G 1 /S phase. Therefore, ING4 expression inhibited cell proliferation and was associated with less colonies being formed. Furthermore, ING4 suppressed hypoxia-inducible factor 1α expression under hypoxic conditions and promoted cell apoptosis. Overall, these results revealed that combining the EGR1 promoter and ING4 ORF using a lentivirus system may be a promising therapeutic strategy with which to enhance radiosensitivity controlled by radiation. However, further studies using in vivo models are required to confirm these findings.

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Section: Discussionmentioning

confidence: 95%

Lentiviral vector with a radiation‑inducible promoter, carrying the ING4 gene, mediates radiosensitization controlled by radiotherapy in cervical cancer cells

Guo

Wang

et al. 2020

Oncol Lett

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“…In colon cancer, PI3K/Akt/NF-κB pathway can be used as upstream factor to activate GLI1, while PI3K/Akt/NF-κB pathway is negatively regulated by PTEN (18,21). Previous studies have shown that ING4 is co-expressed with PTEN (22)(23)(24). In addition, ING5, a homologous protein of ING4, inhibits Akt activity (25).…”

Section: Discussionmentioning

confidence: 99%

Effect of bevacizumab on expression level of GLI1 and ING4 in colon cancer animal model

Suo

Mei

2020

Oncol Lett

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This study aimed to investigate the effect of bevacizumab on GLI1 and ING4 expression in colon cancer animal model. Colon cancer model in rats was induced by azoxymethane (AOM). Bevacizumab was used for the treatment of colon cancer rats. Tumor volume and weight were measured, tumor growth curve was visualized and tumor inhibition rate was calculated. GLI1 and ING4 of colon cancer cells were silencing expressed. Western blot analysis was used to detect the expressions of GLI1, ING4, caspase-3, Bax, β-catenin, Bcl2, PTEN, PI3K, Akt, NF-κB. The apoptosis rate was detected by flow cytometry. MTT assay was used to detect cell activity to get IC 50 value. After AOM induced colon cancer model in rats, the expressions of ING4, caspase-3, Bax and PTEN were downregulated, the expressions of GLI1, β-catenin, Bcl2, PI3K, Akt and NF-κB were upregulated and the apoptosis rate was downregulated. After bevacizumab treatment, the tumor volume and weight decreased, the expressions of ING4, caspase-3, Bax, PTEN were upregulated, the expressions of GLI1, β-catenin, Bcl2, PI3K, Akt, NF-κB were downregulated, and the cell apoptosis rate increased. Cell experiments showed that GLI1 promotes tumor growth and reduces the sensitivity of bevacizumab, while ING4 inhibits tumor growth and increases the sensitivity of bevacizumab. Bevacizumab inhibits the growth of colon cancer tumor by upregulating ING4 and downregulating GLI1. Materials and methods AOM-induced colon cancer model in rats. SD male rats were randomly divided into control group, sham operation group,

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“…The most common modification in the fiber is the addition of an RGD sequence in the HI loop of the knob. This insertion enhances infection of cells that express high levels of α√β3/5 integrins, in particular, dendritic cells and cancer cells. − …”

Section: Penton Complex Capsid Incorporationmentioning

confidence: 99%

Capsid-Incorporation Strategy To Display Antigens for an Alternative Adenoviral Vector Vaccine Approach

2018

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The adenovirus (Ad) is widely used as a vaccine because of its ability to induce a cellular and humoral immune response. In addition, human clinical trials have validated the safety and efficacy of Ad as a vaccine vector. The traditional approach for employing the adenovirus as vaccine is to configure the antigen genes into the expression cassette of the Ad genome. An alternative method for inducing an immune response is the "capsid-incorporation" strategy. This strategy is based upon the incorporation of proteins or peptides into the capsid proteins. This review will focus on the established uses of this approach as well as highlighting the new developments regarding the capsid-incorporation strategy.

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Adenovirus-mediated ING4/PTEN double tumor suppressor gene co-transfer modified by RGD enhances antitumor activity in human nasopharyngeal carcinoma cells

Cited by 11 publications

References 35 publications

Lentiviral vector with a radiation‑inducible promoter, carrying the ING4 gene, mediates radiosensitization controlled by radiotherapy in cervical cancer cells

Lentiviral vector with a radiation‑inducible promoter, carrying the ING4 gene, mediates radiosensitization controlled by radiotherapy in cervical cancer cells

Effect of bevacizumab on expression level of GLI1 and ING4 in colon cancer animal model

Capsid-Incorporation Strategy To Display Antigens for an Alternative Adenoviral Vector Vaccine Approach

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