Adenovirus-mediated gene transfer of basic fibroblast growth factor induces in vitro angiogenesis

Adenoviral gene transfer of basic fibroblast growth factor promotes angiogenesis in rat brain

“…17,18 AxCAJSbFGF has the transgene containing human bFGF cDNA, which does not have a secretory signal sequence. AxCAMAssbFGF also has the transgene containing human bFGF cDNA, but with an artificially fused IL-2 secretory signal sequence.…”

Development of safe and efficient novel nonviral gene transfer using ultrasound: enhancement of transfection efficiency of naked plasmid DNA in skeletal muscle

“…[4][5][6][7][8][9][10][11] Instead of VEGF, other angiogenic growth factors such FGF, HGF and a transcription factor for angiogenesis, HIF (hypoxiainducible factor), have been considered candidates for therapeutic angiogenesis as gene therapy for the treatment of patients with critical limb ischemia. [12][13][14][15][16][17][18][19][20] Although the feasibility of therapeutic angiogenesis using these angiogenic growth factors has been reported in experimental models and human clinical trials, [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] there are still unresolved problems such as undesirable side-effects. Most clinical trials have employed intramuscular injection of naked plasmid DNA despite its low transfection efficiency.…”

“…[8][9][10][11] In addition to VEGF, the utility of gene transfer of other angiogenic growth factors such as fibroblast growth factor (FGF) or hepatocyte growth factor (HGF) has been reported to stimulate collateral formation. [12][13][14][15][16][17][18][19][20] The feasibility of gene therapy using angiogenic growth factors to treat peripheral arterial disease seems to be superior to recombinant protein therapy for the following reasons: (1) It has the potential to maintain an optimally high and local concentration over time. This issue may be critical in the case of arterial gene therapy.…”

“…Recently, many investigators have focused on the adenoviral gene transfer method. 14,15,17,[20][21][22][23][24][25][26] Although the adenoviral vector is very efficient, 14,15,17,[20][21][22][23][24][25][26] there are some theoretical disadvantages such as strong immunogenicity. 27 In addition to efficiency, safety of the gene transfer method is also an important issue, since infusion of adenovirus has recently been reported to cause deleterious side-effects.…”

Adenovirus-mediated ex vivo gene transfer of basic fibroblast growth factor promotes collateral development in a rabbit model of hind limb ischemia

“…)/cell in 3 ml Dulbecco's modified Eagle's minimum essential medium (DMEM, Gibco BRL, Grand Island, NY, USA) with 2% FBS (DMEM-2%). 25 After 1-h incubation, the cells were rinsed twice with PBS and cultured in 10 ml DMEM-2%. The culture medium was changed at 24-h intervals, and the medium and cells were harvested at 1, 4, 7, 10, 14, 21 and 28 days after infection for Western blot analysis, ELISA and 3 H-thymidine incorporation assay.…”