Adenovirus DNA

Landgraf-Leurs, Martina; Green, Maurice

doi:10.1016/0022-2836(71)90457-8

Cited by 51 publications

(4 citation statements)

References 21 publications

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“…Ad2 DNA and Restriction Enzyme Fragments. Ad2 DNA was isolated (Landgraf-Leurs & Green, 1971) and EcoRl restriction fragments were prepared on agarose gels as described (Biittner et al, 1976). £coRI-A fragment (mp 0-58.5) was eluted from the agarose gel, redigested with £coRI, reelectrophoresed, eluted, and immobilized to nitrocellulose filters (Schleicher & Schuell, Inc.) .…”

Section: Methodsmentioning

confidence: 99%

Nucleotide sequences of 5' termini of adenovirus 2 early transforming region Ela and Elb messenger ribonucleic acids

Hashimoto¹,

Wold²,

Brackmann³

et al. 1981

Biochemistry

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Section: Methodsmentioning

confidence: 99%

Nucleotide sequences of 5' termini of adenovirus 2 early transforming region Ela and Elb messenger ribonucleic acids

Hashimoto¹,

Wold²,

Brackmann³

et al. 1981

Biochemistry

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“…Ad2 DNA and restriction fragment -Ad2 DNA was isolated and EcoRI restriction fragments were prepared as described (18,19,15).…”

Section: Methodsmentioning

confidence: 99%

Nucleotide sequences and mapping of novel heterogenous 5′-termini of adenovirus 2 early region 4 mRNA

Hashimoto¹,

Pursley²,

Green³

1981

Nucl Acids Res

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The major 5'-termini of human adenovirus type 2 early gene block 4 mRNA were sequenced. Poly(A+) polyribosomal RNA was isolated from Ad2 early infected cells, the 5'-terminal m7GPPP removed and the 5'-OH of the penultimate 2'-0-methylated nucleotide labeled with [gamma-32P]ATP using polynucleotide kinase. Ad2 E4 mRNA was purified by hybridization to the Ad2 EcoRI-C fragment and was digested with RNase T1. The resulting oligonucleotides were resolved by two dimensional paper electrophoresis-homochromatography. Four major and 3-4 minor 5'-terminal sequences were identified and characterized. The sequence of the 5'-terminal structures of the major four termini are: (1) m7GpppUmU(m)UUACACUGp, (2) m7GpppUmU(m)UACACUGp, (3) m7GpppUmU(m)ACACUGp, and (4) m7Gppp(m6)AmC(m)ACUGp. These major 5'-terminal sequences were aligned with nucleotide 325, 326, 327, and 329 from the righthand end of the known Ad2 DNA sequence (1) in the region mapped as the 5'-terminus of E4 mRNA by electron microscopy (2,3) and S1 nuclease-gel (4) mapping. Two potential ribosomal binding sites and an initiator codon were found at 40 to 65 nucleotides and about 80 nucleotides, respectively, from these heterogenous 5'-termini. Ad2 E4 major mRNA species appear to be unique since mRNA molecules initiate at a pyrimidine, perhaps by RNA polymerase stuttering, or they are products of an unusual type of RNA processing.

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“…1B and C suggests that the heavy-strand peak is skewed toward the light-strand peak, and vice versa. Therefore, fractions containing the heavy or light strands were separately pooled and self-annealed for [3][4][5] hr. The self-annealed strands were then collected on nitrocellulose membrane filters.…”

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Evidence for a Transcription-Control Region of Simian Virus 40 in the Adenovirus 2—Simian Virus 40 Hybrid, Ad2 ⁺ ND ₁

Patch

Lewis

Levine

1972

Proc. Natl. Acad. Sci. U.S.A.

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The complementary DNA strands of the nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, Ad2+ND,, were separated by isopycnic banding in a CsCl density gradient in the presence of synthetic polyribonucleotides. Separated strands were used in DNA-RNA hybridization reactions with RNA from cells productively infected by Ad2 or SV40, and with complementary SV40 RNA transcribed asymmetrically in vitro. About five times as much Ad2 RNA hybridized to the SV40 cRNA-I hybridized with only one strand (the strand) which was selcarated from the unhybridized strand (the "-+" strand) by hydroxyapatite chromatography.Using seIzarated strands, Khoury et al. have shown that about one-third of the -strand of SV40 DNA is transcribed before viral )-AN synithesis begins, while none of the + strand is transcribed during the early phase of infection. On the other hand, the RNA transcribed in the late phase of infection is homologous with one-third of the -strand and two-thirds of the + strand. Thus, the early DNA template occul)ies about one-third of the -strand and the late D)NA template occupies about two-thirds of the + strand.The nondefective Ad2-SV40 hybrid virus Ad2+NDj (14) contains about 10

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Adenovirus DNA

Cited by 51 publications

References 21 publications

Nucleotide sequences of 5' termini of adenovirus 2 early transforming region Ela and Elb messenger ribonucleic acids

Nucleotide sequences of 5' termini of adenovirus 2 early transforming region Ela and Elb messenger ribonucleic acids

Nucleotide sequences and mapping of novel heterogenous 5′-termini of adenovirus 2 early region 4 mRNA

Evidence for a Transcription-Control Region of Simian Virus 40 in the Adenovirus 2—Simian Virus 40 Hybrid, Ad2 ⁺ ND ₁

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Adenovirus DNA

Cited by 51 publications

References 21 publications

Nucleotide sequences of 5' termini of adenovirus 2 early transforming region Ela and Elb messenger ribonucleic acids

Nucleotide sequences of 5' termini of adenovirus 2 early transforming region Ela and Elb messenger ribonucleic acids

Nucleotide sequences and mapping of novel heterogenous 5′-termini of adenovirus 2 early region 4 mRNA

Evidence for a Transcription-Control Region of Simian Virus 40 in the Adenovirus 2—Simian Virus 40 Hybrid, Ad2 + ND 1

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Evidence for a Transcription-Control Region of Simian Virus 40 in the Adenovirus 2—Simian Virus 40 Hybrid, Ad2 ⁺ ND ₁