Adenovirus and minute virus of mice DNAs are localized at the nuclear periphery

Moen, Phillip T.; Fox, Elizabeth; Bodnar, John W.

doi:10.1093/nar/18.3.513

Cited by 24 publications

(16 citation statements)

References 20 publications

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“…Thus, the E1B transcription unit is exposed to a different population of trans-regulatory factors early versus late after infection. Recent electron microscope work has localized adenovirus DNA in the late phase to discrete foci or 'replication factories' in the nucleus (Moen et al, 1990) and thus one could postulate that two templates in the same cell can be in separate nuclear compartments. Several groups have shown that adenovirus DNA is linked tightly to the nuclear matrix throughout infection and that this linkage is important for viral transcription and replication (Bodnar et al, 1989;Schaack et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…Several groups have shown that adenovirus DNA is linked tightly to the nuclear matrix throughout infection and that this linkage is important for viral transcription and replication (Bodnar et al, 1989;Schaack et al, 1990). Recent electron microscope work has localized adenovirus DNA in the late phase to discrete foci or 'replication factories' in the nucleus (Moen et al, 1990) and thus one could postulate that two templates in the same cell can be in separate nuclear compartments. Additionally there is evidence that not only transcription but also processing of mRNA is believed to occur on the nuclear matrix and in discrete places (Lawrence et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

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DNA template effect on RNA splicing: two copies of the same gene in the same nucleus are processed differently.

Adami

Babiss

1991

The EMBO Journal

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Many cellular and viral genes are parts of complex transcription units containing multiple splicing choices. During the course of an adenoviral replicative cycle, different spliced versions of a single gene predominate, depending on the stage of infection. This is true for several adenoviral genes. In this paper we show for the viral E1B transcription unit that splice site usage regulates this process. The change in alternative splicing in this system does not depend on the sequence of the transcribed genes. Non‐adenoviral genes, such as the SV40 early region and the polyoma early region, which normally show little or no regulation of spliced RNA product formation, become regulated for mRNA production after insertion into the adenoviral genome. Additional studies show that E1B splicing regulation in adenovirus is a cis effect. Staggered infections using two discernable viral genomes resulted in a situation where both early and late genomes exist in the same nucleus. Neither genome was able to impose its regulated splicing pattern on the other, indicating that the cue for the switch in viral gene splicing is not directly dependent on global changes in trans‐acting splicing factors. This suggests a model where the signal for changes in RNA processing for the E1B gene is linked to the state of the DNA template or its localization within nuclear subcompartments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

DNA template effect on RNA splicing: two copies of the same gene in the same nucleus are processed differently.

Adami

Babiss

1991

The EMBO Journal

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“…The NM is involved in many important cellular activities, such as transcription, mRNA splicing, mRNA transport, chromatin organization, and DNA replication (6,28,35,49). Therefore, it is not surprising that sites of Ad replication and transcription are discretely localized to foci in the nuclear substructure (11,37). The interaction of Ad pTP with the NM may link these cellular processes with the viral life cycle.…”

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confidence: 99%

Tyrosine kinase-dependent release of an adenovirus preterminal protein complex from the nuclear matrix

Angeletti

Engler

1996

J Virol

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Adenovirus (Ad) replicative complexes form at discrete sites on the nuclear matrix (NM) through the interaction of Ad preterminal protein (pTP). The NM is a highly salt-resistant fibrillar network which is known to anchor transcription, mRNA splicing, and DNA replication complexes. Incubation of rATP with NM to which pTP was bound caused the release of pTP as a pTP-NM complex with a size of 220 to 230 kDa; incubation with 5 adenylylimidodiphosphate (rAMP-PNP) showed no significant release, indicating that rATP hydrolysis was required. With NM extracts, it was shown that a pTP-NM complex which was capable of binding Ad origin DNA could be reconstituted in vitro. A number of high-molecular-weight NM proteins ranging in size from 120 to 200 kDa were identified on Far Western blots for their ability to bind pTP. rATP-dependent release of pTP from the NM was inhibited in a dose-dependent fashion by the addition of tyrosine kinase inhibitors, such as quercetin, methyl-2,5-dihydroxycinnamate, or genistein. NM-mediated phosphorylation of a poly(Glu, Tyr) substrate was also significantly abrogated by the addition of these compounds. rATP-dependent release of Ad DNA termini bound to the NM via pTP was also blocked by the addition of these inhibitors. These results indicate that a tyrosine kinase mechanism controls the release of pTP from its binding sites on the NM. These data support the concept that phosphorylation may play a key role in the modulation of pTP binding sites on the NM.

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“…AAV-2 then uses the cellular replication factors and factors involved in RNA transcription and processing recruited to these centers for its own propagation. Interestingly, the genome replication of the autonomous minute virus of mice (MVM) is increased strongly by Ad2 infection in HeLa cells (15) accompanied by a recompartmentalization of the MVM DNA from the nucleolus to the adenoviral replication centers (15,33). In contrast to the situation with B19V, however, 293 cells are able to replicate MVM DNA quite efficiently in the absence of adenovirus, and no extra stimulation is observed by providing additional adenoviral functions, although recompartmentalization of the MVM DNA is also observed (15).…”

Section: Discussionmentioning

confidence: 99%

Roles of E4orf6 and VA I RNA in Adenovirus-Mediated Stimulation of Human Parvovirus B19 DNA Replication and Structural Gene Expression

Winter

Kietzell

Heilbronn

et al. 2012

J Virol

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Despite its very narrow tropism for erythroid progenitor cells, human parvovirus B19 (B19V) has recently been shown to replicate and form infectious progeny virus in 293 cells in the presence of early adenoviral functions provided either by infection with adenovirus type 5 or by addition of the pHelper plasmid encoding the E2a, E4orf6, and VA RNA functions. In the present study we dissected the individual influence of these functions on B19V genome replication and expression of structural proteins VP1 and VP2. We show that, in the presence of the constitutively expressed E1A and E1B, E4orf6 alone is able to promote B19V DNA replication, resulting in a concomitant increase in VP expression levels. The stimulatory effects of E4orf6 require the integrity of the BC box motifs, which target cellular proteins such as p53 and the Mre11 DNA repair complex for proteosomal degradation through formation of an E3 ubiquitin ligase complex with E1B. VA RNA also strongly induces VP expression but, in contrast to E4orf6, in a replication-independent manner. This stimulation could be attributed exclusively to the VA I RNA transcript and does not involve major activating effects at the level of the B19V p6 promoter, but the nucleotide residues required for the welldefined pathway of VA I RNA mediated stimulation of translation through functional inactivation of protein kinase R. These data show that the cellular pathways regulating B19V replication may be very similar to those governing the productive cycle of the helper-dependent parvoviruses, the adeno-associated viruses.

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Adenovirus and minute virus of mice DNAs are localized at the nuclear periphery

Cited by 24 publications

References 20 publications

DNA template effect on RNA splicing: two copies of the same gene in the same nucleus are processed differently.

DNA template effect on RNA splicing: two copies of the same gene in the same nucleus are processed differently.

Tyrosine kinase-dependent release of an adenovirus preterminal protein complex from the nuclear matrix

Roles of E4orf6 and VA I RNA in Adenovirus-Mediated Stimulation of Human Parvovirus B19 DNA Replication and Structural Gene Expression

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