Adenosine-Prefabricated Adipose Tissue Improves Fat Graft Survival by Promoting VEGF-Dependent Angiogenesis

Chang, Jiyeon; Song, Woo Jin; Soedono, Shindy; Sharlene, Sharlene; Kim, Yeong Jin; Choi, Chang Yong; Cho, Kae Won

doi:10.1007/s13770-022-00470-4

Cited by 6 publications

(5 citation statements)

References 35 publications

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“…Besides MSC cell behavior itself, dynamic regulation of ASCs with niche is crucial factor to determine the fat graft survival rate. Previously, our group reported that VEGF-induced angiogenesis and lipolysis play an important role in regulating fat graft survival [ 53 , 54 ]. Recent studies have shown that biophysical signals can regulate the immunomodulatory functions of ASCs through cytokine secretion [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Chun

Chang

Soedono

et al. 2022

IJMS

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Cell-assisted lipotransfer (CAL), defined as co-transplantation of aspirated fat with enrichment of adipose-derived stem cells (ASCs), is a novel technique for cosmetic and reconstructive surgery to overcome the low survival rate of traditional fat grafting. However, clinically approved techniques for increasing the potency of ASCs in CAL have not been developed yet. As a more clinically applicable method, we used mechanical stress to reinforce the potency of ASCs. Mechanical stress was applied to the inguinal fat pad by needling . Morphological and cellular changes in adipose tissues were examined by flow cytometric analysis 1, 3, 5, and 7 days after the procedure. The proliferation and adipogenesis potencies of ASCs were evaluated. CAL with ASCs treated with mechanical stress or sham control were performed, and engraftment was determined at 4 weeks post-operation. Flow cytometry analysis revealed that mechanical stress significantly increased the number as well as the frequency of ASC proliferation in fat. Proliferation assays and adipocyte-specific marker gene analysis revealed that mechanical stress promoted proliferation potential but did not affect the differentiation capacity of ASCs. Moreover, CAL with cells derived from mechanical stress-treated fat increased the engraftment. Our results indicate that mechanical stress may be a simple method for improving the efficacy of CAL by enhancing the proliferation potency of ASCs.

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Section: Resultsmentioning

confidence: 99%

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Chun

Chang

Soedono

et al. 2022

IJMS

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“…Our previous study demonstrated the effect of adenosine on engraftment using normal adipose tissue [4]. We chose to use epididy-mal visceral adipose tissue due to its ease of access and the larger quantity available compared to inguinal subcutaneous fat.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, adenosine-an endogenous substance-increases its own production under hypoxic conditions, interacting with adenosine receptors to regulate inflammation and protect cells from diverse stressors [3]. Our research team has demonstrated that prefabrication, as opposed to direct injection, increases angiogenesis and improves graft survival [4].…”

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confidence: 99%

Adenosine washing improves the retention rate of fat grafts under conditions of obesity

Kim,

Cha,

Park

et al. 2024

Arch Aesthetic Plast Surg

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Background Fat grafting is a commonly employed aesthetic procedure for contour enhancement. However, outcome prediction is challenging due to the complex regeneration and remodeling processes involved. We investigated whether adenosine improves engraftment and fat graft survival under conditions of obesity.Methods Fat was harvested from mice fed a high-fat diet. This fat was washed with either Krebs-Ringer bicarbonate HEPES buffer (the vehicle group) or a buffer containing 500 nM adenosine (the adenosine wash group). Subsequently, the fat was transplanted into normal mice at 0.2 mL per mouse. In both groups, 50% of the mice were sacrificed at 1 week and the remainder at 4 weeks post-transplantation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was conducted during week 1. In week 4, micro-computed tomography, immunofluorescence staining, and RT-qPCR were performed. A sample of the initially harvested fat was set aside for lipolysis assay.Results Adenosine washing improved fat graft retention volumes by up to 50%. One week post-transplantation, the expression of adipogenic and angiogenic genes was found to be upregulated in the adenosine wash group. After 4 weeks, immunofluorescence staining revealed greater adipocyte integrity and an increased number of vessels. Furthermore, adenosine appeared to modulate inflammation by stabilizing the lipolysis rate.Conclusions Adenosine washing increased the fat graft survival rate under conditions of obesity. Clinically, this suggests a simple, cost-effective adjuvant method for improving fat graft survival in individuals with obesity. Further research is warranted to elucidate the underlying mechanisms and explore the applicability of this technique for autologous transplantation.

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“…However, the results of imaging studies in humans indicate that adenosine, in addition to the aforementioned effects, and lowering the concentration of free fatty acids, glycerol, and triglycerides in plasma, is also important in the activation of BAT [ 65 ]. In other studies, adenosine has been shown to promote alternative macrophage activation, prevent adipose tissue inflammation, and play a role in maintaining glucose homeostasis [ 66 ]. Taking into account its broad-spectrum effect, it should be considered as an active substance bound to adenosine receptors that would be most effective in reducing body weight in humans, but also harmless regarding the proper functioning of the organism [ 63 , 64 , 65 , 66 ].…”

Section: Methods Of the Futurementioning

confidence: 99%

“…In other studies, adenosine has been shown to promote alternative macrophage activation, prevent adipose tissue inflammation, and play a role in maintaining glucose homeostasis [ 66 ]. Taking into account its broad-spectrum effect, it should be considered as an active substance bound to adenosine receptors that would be most effective in reducing body weight in humans, but also harmless regarding the proper functioning of the organism [ 63 , 64 , 65 , 66 ].…”

Section: Methods Of the Futurementioning

confidence: 99%

Modern and Non-Invasive Methods of Fat Removal

Piłat,

Szpila,

Stojko

et al. 2023

Medicina

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Adipocytes accumulate triacylglycerols as an energy store, thereby causing an increase in the adipose tissue volume. Weight gain can be prevented through damage to the adipocyte structure or an increase in the body’s metabolic rate. Commonly used methods to disintegrate the cell membrane of adipocytes include injection lipolysis, cryolipolysis, ultrasonic lipolysis, radiofrequency lipolysis, laser lipolysis, carboxytherapy, and lipolysis using an electromagnetic field. The names of these methods suggest which substances are being used, and their main advantages are a very low invasiveness, as well as effectiveness. However, new discoveries in medicine, along with individuals’ desire to improve their appearance, have resulted in numerous studies on more ways of reducing body fat. Great potential is seen in beige adipocytes, which can be transformed, i.e., “recruited” from white adipocytes, or synthesized de novo; they also show thermogenic properties. One of the stimuli inducing the formation of beige adipocytes is cold and B3-adrenergic stimulation. Based on these findings, the researchers created, for example, cooling clothing. Additionally, curcumin and natural anthocyanins have proven to be helpful in the treatment of obesity and diabetes, by stimulating the secretion of glucagon-like peptide-1, and inducing the formation of beige adipocytes. Another study showed that the conversion of white adipose tissue is indirectly influenced by interleukin-6 secreted by the muscles, the expression of which is increased in people actively exercising. Moreover, there is potential in adenosine analogs, fenoldopam, rhubarb, the herbal extract Ephedra sinica Stapf, electroacupuncture simulation, and the drug CBL-514. Despite knowledge and experience, the ideal method for a quick and noticeable, but safe and non-invasive reduction of body fat has not been found yet. The research conducted nowadays may bring us closer to the development of a universal method, and turn out to be a breakthrough in the fight against overweight and obesity.

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Adenosine-Prefabricated Adipose Tissue Improves Fat Graft Survival by Promoting VEGF-Dependent Angiogenesis

Cited by 6 publications

References 35 publications

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Adenosine washing improves the retention rate of fat grafts under conditions of obesity

Modern and Non-Invasive Methods of Fat Removal

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