Adenosine nucleotides in rat bone measured by ion-pair reversed-phase high-performance liquid chromatography: effect of hemorrhagic shock, with and without retransfusion of blood

Manoharan, M.; Schwille, R.; Schwille, P. O.

doi:10.1016/s0378-4347(01)00307-3

Cited by 9 publications

(5 citation statements)

References 17 publications

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“…Different sorbents are used for removing different interferences. ATP, ADP, and AMP are not retained by C18 resins due to their high polarity (16 ). Moreover, C18 resins can efficiently absorb lipids and pigments in RJ, as outlined in the manufacturer's recommendations (21 ).…”

Section: Resultsmentioning

confidence: 99%

“…High-performance liquid chromatography (HPLC) is currently the most commonly used separation technique for adenosine phosphate, used in combination with detection by ultraviolet light , − and mass spectrometry , . In particular, as a conventional method, HPLC can be performed using standard equipment in many laboratories and is simple, sensitive, and suitable for monitoring adenosine phosphate.…”

Section: Introductionmentioning

confidence: 99%

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Online Cleanup of Accelerated Solvent Extractions for Determination of Adenosine 5′-Triphosphate (ATP), Adenosine 5′-Diphosphate (ADP), and Adenosine 5′-Monophosphate (AMP) in Royal Jelly Using High-Performance Liquid Chromatography

Xue

Wang

Zhou

et al. 2009

J. Agric. Food Chem.

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Determination of the levels of adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), and adenosine 5'-monophosphate (AMP) in royal jelly is important for the study of its pharmacological activities, health benefits, and adenosine phosphate degradation. In this study was developed a novel method to determine ATP, ADP, and AMP levels in royal jelly using accelerated solvent extraction (ASE) followed by online cleanup and high-performance liquid chromatography (HPLC) with diode array detection (DAD). The optimum extraction conditions were obtained using an 11 mL ASE cell, ethanol/water (5:5 v/v) as the extraction solvent, 1500 psi, 80 degrees C, a 5 min static time, and a 60% flush volume. Optimum separation of the three compounds was achieved in <25 min using a Waters XBridge Shield RP18 column with 0.05 mol L(-1) NH(4)H(2)PO(4) (pH 5.70) and acetonitrile as the mobile phase. Detection was performed at 257 nm. The method was sensitive (LOD

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Online Cleanup of Accelerated Solvent Extractions for Determination of Adenosine 5′-Triphosphate (ATP), Adenosine 5′-Diphosphate (ADP), and Adenosine 5′-Monophosphate (AMP) in Royal Jelly Using High-Performance Liquid Chromatography

Xue

Wang

Zhou

et al. 2009

J. Agric. Food Chem.

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“…5). Recent studies have employed conditions with run times between~5-20 min (e.g., [14,19,20]). Although the sensitivity of our UV detector precluded the possibility of detecting adenylates at levels present in our micro-samples (e.g., N 100 pmol ATP), these conditions appear appropriate for detecting adenylate nucleotides in larger biological samples.…”

Section: Resultsmentioning

confidence: 99%

Quantitating adenylate nucleotides in diverse organisms

Napolitano

Shain

2005

Journal of Biochemical and Biophysical Methods

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“…Therefore, much attention has been paid to the selective analysis of phosphorylated substances. As a chromatographic separation method, ion-pair reversed-phase HPLC was successfully applied to the analysis of adenosine phosphate compounds [1], and immobilized metal affinity chromatography (IMAC) is employed in phosphopeptide-selective analysis [2]. Recently, titanium dioxide (titania, TiO 2 ) has been found to show uniquely amphoteric ion-exchange properties, and applied as an alternative support material to silica in the field of HPLC packings [3,4].…”

Section: Introductionmentioning

confidence: 99%

Titania-coated monolithic silica as separation medium for high performance liquid chromatography of phosphorus-containing compounds

Miyazaki¹,

Miah

Morisato

et al. 2005

J. Sep. Science

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A method of preparing titania-coated monolithic silica stationary phase has been developed to achieve liquid chromatographic separation of phosphorus-containing compounds, which have recently been attracting increasing attention in biochemical research. The titania-coated silica columns exhibited efficient separation with low pressure drop, which is a typical feature of monolithic structures, and also possessed phospho-selectivity, which is a unique property of the titania surface. The material characteristics of titania-coated monolithic silica were examined, and then resin-clad columns were applied to the HPLC analysis of phosphorylated compounds. Highly efficient separation of phosphorylated substances indicated that the novel titania-coated monolithic silica column will find applications as a useful tool in the field of biochemistry, especially in post-genomic analyses.

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Adenosine nucleotides in rat bone measured by ion-pair reversed-phase high-performance liquid chromatography: effect of hemorrhagic shock, with and without retransfusion of blood

Cited by 9 publications

References 17 publications

Online Cleanup of Accelerated Solvent Extractions for Determination of Adenosine 5′-Triphosphate (ATP), Adenosine 5′-Diphosphate (ADP), and Adenosine 5′-Monophosphate (AMP) in Royal Jelly Using High-Performance Liquid Chromatography

Online Cleanup of Accelerated Solvent Extractions for Determination of Adenosine 5′-Triphosphate (ATP), Adenosine 5′-Diphosphate (ADP), and Adenosine 5′-Monophosphate (AMP) in Royal Jelly Using High-Performance Liquid Chromatography

Quantitating adenylate nucleotides in diverse organisms

Titania-coated monolithic silica as separation medium for high performance liquid chromatography of phosphorus-containing compounds

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