Adenosine Deaminase in Lymphocytes and its Electrophoretic Separation

Wüst, Hans Joachim

doi:10.1159/000152454

Hum Hered

1971

DOI: 10.1159/000152454

|View full text |Cite

Adenosine Deaminase in Lymphocytes and its Electrophoretic Separation

Hans Joachim Wüst¹

Abstract: The adenosine deaminase polymorphism was found in lymphocyte lysates. Lymphocytes exhibited an additional ADA isozyme distinguished from other ADA isozymes by its lack of free -SH groups. It was suggested that this new isozyme is controlled by a second genetic locus.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1974

2001

Publication Types

Select...

Article5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 13 publications

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Polychlorinated polyamides. II. Synthesis and characterization of polyamides derived from 2, 5‐dichloroterephthaloyl dichloride and perchloroterephthaloyl dichloride

Díaz

Gargallo

Lizana

et al. 1979

J. Polym. Sci. Polym. Chem. Ed.

View full text Add to dashboard Cite

New thermally stable polyamides were prepared by interfacial polymerization of the title compounds and various aliphatic and aromatic diamines. The polymers were characterized by infrared spectrometry, elemental analysis, and differential scanning calorimetry. The molecular weights of the polymers were estimated by viscosity measurements. The influence of the halogen content on the thermal properties and degree of polymerization of the polymers was studied. One of the polyamides was fractionated to determine the degree of polydispersity.

show abstract

Polychlorinated polyamides. II. Synthesis and characterization of polyamides derived from 2, 5‐dichloroterephthaloyl dichloride and perchloroterephthaloyl dichloride

Díaz

Gargallo

Lizana

et al. 1979

J. Polym. Sci. Polym. Chem. Ed.

View full text Add to dashboard Cite

show abstract

Adenosindesaminase-Mangel bei prim�ren Immundefizienzen

Belohradsky¹,

Hennig²,

Marget³

et al. 1976

Klin Wochenschr

View full text Add to dashboard Cite

The occurrence of severe combined immunodeficiency (SCID) with adenosine deaminase (ADA) deficiency in erythrocytes has been reported in 14 patients. Enzyme deficiency may result in early depression of the lymphatic system. ADA is detectable in different tissues by photometric and electrophoretic methods. The gene locus for ADA has been localised on chromosome 20. Studies on the enzyme defect in different forms of primary immunodeficiencies led to the description of a well defined nosological entity. New aspects can be expected in the fields of pathogenesis, prenatal diagnosis, genetic councelling, and possibly therapeutic trials.

show abstract

Adenosine Deaminase Activity and its Isoenzyme Pattern in Patients with Juvenile Rheumatoid Arthritis and Systemic Lupus Erythematosus

et al. 2001

View full text Add to dashboard Cite

Adenosine deaminase (ADA) is involved in purine metabolism and plays a significant role in the mechanisms of the immune system. The aim of this study was to investigate the activity of total ADA (tADA) and its isoenzymes ADA1 and ADA2 in serum and peripheral blood lymphocytes (PBLs) of children with juvenile rheumatoid arthritis (JRA) and systemic lupus erythematosus (SLE) in different phases of the diseases. The study comprised 34 patients with rheumatic disease, 24 with JRA and 10 with SLE, and 64 healthy controls. The tADA activity and its isoenzymes were measured in serum and PBLs of all patients by the method of Giusti and by the presence or absence of EHNA (erythro-9-(2-hydroxy-3-nonyl)adenine) during the active phase of the disease (before treatment), as well as during remission and relapse. Our data show that increased tADA activity in the serum and PBLs of patients with JRA and SLE is correlated mainly to increased levels of ADA2 activity in serum and ADA1 activity in PBLs. It also closely correlates with clinical disease activity and relapse. The cause of this increased tADA/ADA2 activity in serum and tADA/ADA1 activity in PBLs in JRA and SLE remains to be elucidated. Nevertheless, it may be noted that the measurement of tADA activity, together with ADA2 activity in serum and tADA with ADA1 activity in PBLs, could offer a biochemical approach to the assessment of the pathophysiology of JRA and SLE. Also, tADA and its isoenzymes could be used as alternative parameters representing disease activity.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Adenosine Deaminase in Lymphocytes and its Electrophoretic Separation

Abstract: The adenosine deaminase polymorphism was found in lymphocyte lysates. Lymphocytes exhibited an additional ADA isozyme distinguished from other ADA isozymes by its lack of free -SH groups. It was suggested that this new isozyme is controlled by a second genetic locus.

Cited by 13 publications

References 8 publications

Polychlorinated polyamides. II. Synthesis and characterization of polyamides derived from 2, 5‐dichloroterephthaloyl dichloride and perchloroterephthaloyl dichloride

Polychlorinated polyamides. II. Synthesis and characterization of polyamides derived from 2, 5‐dichloroterephthaloyl dichloride and perchloroterephthaloyl dichloride

Adenosindesaminase-Mangel bei prim�ren Immundefizienzen

Adenosine Deaminase Activity and its Isoenzyme Pattern in Patients with Juvenile Rheumatoid Arthritis and Systemic Lupus Erythematosus

Contact Info

Product

Resources

About