Adenosine and TNF-α exert similar inotropic effect on heart cultures, suggesting a cardioprotective mechanism against hypoxia

El-Ani, Dalia; Zimlichman, Reuven; Mashiach, Yaakov; Shainberg, Asher

doi:10.1016/j.lfs.2007.06.030

Cited by 12 publications

(19 citation statements)

References 59 publications

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“…There have been many reports describing the relationship between the two myocardial enzymes and cardioprotective effects [13][14][15]. The CK and LDH are released into the blood stream after myocardial injury.…”

Section: Discussionmentioning

confidence: 99%

Cardioprotective effects of <i>Dan-Yang-Fu-Xin</i> decoction on chronic heart failure in rats

Guan¹,

Chen²,

Yang³

2016

Trop. J. Pharm Res

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Purpose: To evaluate the cardioprotective effects and possible mechanisms of Dan-Yang-Fu-Xin decoction (DYFX) in a rat chronic heart failure (CHF). Methods: A CHF rat model induced by ligation of the left anterior descending coronary artery was used to investigate the cardioprotective effects of DYFX. After intragastric administration for 8 weeks, several functional cardiac indices, including fractional shortening (FS), ejection fraction (EF), heart rate (HR) and cardiac output (CO) were assessed by ultrasound examination. Subsequently, inflammatory markers, viz, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), myocardial enzymes, namely, lactate dehydrogenase (LDH) and creatine kinase (CK), were also assessed by enzyme-linked immunosorbent assay (ELISA). Results: Intragastric administration of DYFX (200, 400 and 600 mg/kg) significantly reversed the decrease in body weight and increase in cardiac weight (p < 0.05) induced by CHF. Treatment with DYFX also significantly reversed EF, FS, HR, and CO changes in CHF rats. In addition, DYFX inhibited the two inflammatory cytokines and myocardial enzymes (CK and LDH)

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Section: Discussionmentioning

confidence: 99%

Cardioprotective effects of <i>Dan-Yang-Fu-Xin</i> decoction on chronic heart failure in rats

Guan¹,

Chen²,

Yang³

2016

Trop. J. Pharm Res

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“…The hearts were minced into small fragments and then gently agitated in RDB, a solution of proteolytic enzymes prepared from figtree extract (Biological Institute, Ness-Ziona, Israel). RDB was diluted 1:100 in Ca 2+ -and Mg 2+ -free PBS at 25°C and incubated with the heart fragments for several cycles of 10 min each, as previously described (El-Ani et al, 2007). Dulbecco's modified Eagle's medium, supplemented with 10% inactivated horse serum (Biological Industries, Kibbutz Beit Haemek, Israel) and 0.5% chick embryo extract, was added to the supernatant containing a suspension of dissociated cells.…”

Section: Preparation Of Heart Culturesmentioning

confidence: 99%

“…Experiments were performed as previously described (El-Ani et al, 2007). Heart culture dishes were pre-incubated for 20 min with or without 10 μM rapamycin in glucose-free PBS at 37°C.…”

Section: Hypoxia and Reoxygenation (H/r)mentioning

confidence: 99%

“…Measurements were performed as previously described (El-Ani et al, 2007) according to Sigma protocol. After H/R of cardiomyocytes in 96 well plates, 10 μl of the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) solution (5 mg/ml) was transferred to 100 μl PBS of each well.…”

Section: Mtt Assaymentioning

confidence: 99%

“…Skinning procedure and 45 Ca 2+ accumulation in the SR Experiments were performed as previously described (El-Ani et al, 2007). I) Skinning procedure: Cell were incubated in 1 ml skinning solution (140 mM KCl, 20 mM HEPES buffer, 10 mM EGTA, 50 μg/ml saponin, 5 mM NaN 3 , 5 mM potassium oxalate, 1 μM ruthenium red, pH 7.4) at 25°C for 20 min.…”

Section: Immunostaining After Hypoxia and Rapamycin Treatmentmentioning

confidence: 99%

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Rapamycin (sirolimus) protects against hypoxic damage in primary heart cultures via Na+/Ca2+ exchanger activation

El-Ani¹,

Stav²,

Guetta³

et al. 2011

Life Sciences

Self Cite

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The role of the T cell in age-related inflammation

Macaulay

Akbar

Henson

2012

AGE

105

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Ageing is accompanied by alterations to T-cell immunity and also by a low-grade chronic inflammatory state termed inflammaging. The significance of these phenomena is highlighted by their being predictors of earlier mortality. We have recently published that the proinflammatory cytokine TNFα is a strong inducer of CD4 + T-cell senescence and T-cell differentiation, adding to the growing body of literature implicating proinflammatory molecules in mediating these critical age-related T-cell alterations. Moreover, the inflammatory process is also being increasingly implicated in the pathogenesis of many common and severe age-related diseases, including cancer, cardiovascular diseases and type 2 diabetes. Furthermore, major age-related risk factors for poor health, such as obesity, stress and smoking, are also associated with an upregulation in systemic inflammatory markers. We propose the idea that the ensuing inflammatory response to influenza infection propagates cardiovascular diseases and constitutes a major cause of influenzarelated mortality. While inflammation is not a negative phenomenon per se, this age-related dysregulation of inflammatory responses may play crucial roles driving age-related pathologies, T-cell immunosenescence and CMV reactivation, thereby underpinning key features of the ageing process.

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Adenosine and TNF-α exert similar inotropic effect on heart cultures, suggesting a cardioprotective mechanism against hypoxia

Cited by 12 publications

References 59 publications

Cardioprotective effects of <i>Dan-Yang-Fu-Xin</i> decoction on chronic heart failure in rats

Cardioprotective effects of <i>Dan-Yang-Fu-Xin</i> decoction on chronic heart failure in rats

Rapamycin (sirolimus) protects against hypoxic damage in primary heart cultures via Na+/Ca2+ exchanger activation

The role of the T cell in age-related inflammation

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