1988
DOI: 10.1152/ajpheart.1988.255.3.h554
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Adenosine and hypoxia stimulate proliferation and migration of endothelial cells

Abstract: The proliferation of bovine aortic or coronary venular endothelial cells (EC) in vitro was stimulated by the addition of adenosine (0.5 or 5.0 microM) to the culture medium. Cell counts of adenosine-treated aortic EC were 23-76% and coronary venular EC 19-52% greater than nontreated controls. Because adenosine is known to be released by hypoxic tissues, cell proliferation was quantitated when aortic EC were grown at 2% O2. Cell counts were 41-102% greater under hypoxic conditions than when cells were grown at … Show more

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Cited by 111 publications
(91 citation statements)
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“…Next, the proliferation of BAECs was determined by [ 3 H]-methylthymidine incorporation assay. The DNA synthesis of cells was increased under hypoxia as reported previously, 31 but the FK228 (10 ng/ml) effectively suppressed the hypoxia-stimulated DNA synthesis (Fig. 2a).…”
Section: Fk228 Showed Potent Antiangiogenic Activities In Vitrosupporting
confidence: 86%
“…Next, the proliferation of BAECs was determined by [ 3 H]-methylthymidine incorporation assay. The DNA synthesis of cells was increased under hypoxia as reported previously, 31 but the FK228 (10 ng/ml) effectively suppressed the hypoxia-stimulated DNA synthesis (Fig. 2a).…”
Section: Fk228 Showed Potent Antiangiogenic Activities In Vitrosupporting
confidence: 86%
“…That the enzyme activity is required for angiogenic activity was shown with three active site mutants that maintained their secondary structure, as determined by their capacity to dimerize, but had reduced TP activity. The mutants did not exhibit angiogenic activity in the rat sponge model when assayed at the (25) and to be angiogenic in vivo (26). TP hydrolyzes thymidine to 2'-deoxy-D-ribose 1-phosphate and thymine.…”
Section: Discussionmentioning
confidence: 95%
“…Neovascularization is of relevance during inflammation, wound healing, and tumor growth and the study of these processes has resulted in the identification of a wide variety of angiogenesis inducers (3,4,15,19). The morphogenetic program of the neovascular response requires multiple steps, and an increase in blood flow and vasodilation of the parent venule before the emergence of the first capillary sprouts are important steps (6,32).…”
Section: Methodsmentioning
confidence: 99%