Adenosine A1 receptors modulate the Na+-Hypertonicity induced glutamate release in hypothalamic glial cells

Braga, Danielle Valente; Picanço-Diniz, Domingos Luiz Wanderley; Oliveira, Karen Renata Matos; Luz, Waldo Lucas; Moraes, Suellen Alessandra Soares de; Passos, Adelaide da Conceição Fonseca; Batista, Evander de Jesus Oliveira; Grisólia, Alan Araújo; Herculano, Anderson Manoel

doi:10.1016/j.neuint.2019.02.013

Cited by 3 publications

(2 citation statements)

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“…In this study, we found that XYS treatment downregulated the increases in the protein expression levels of A2AR in the striatum of rats induced by CRS. Studies have found that A2AR mediates phosphorylation and upregulation of the extracellular signal ERK1/2, resulting in increased Glu release ( Braga et al, 2019 ; Köfalvi et al, 2020 ), causing high NF-κB expression and promoting microglial proliferation and activation ( Mohamed et al, 2016 ). Our data also showed that the expression levels of p-ERK and NF-κB p65 in the striatum of rats in the CRS model group were increased, whereas XYS treatment downregulated these protein levels.…”

Section: Discussionmentioning

confidence: 99%

Xiaoyaosan Ameliorates Chronic Restraint Stress-Induced Depression-Like Phenotype by Suppressing A2AR Signaling in the Rat Striatum

Zhu

Yang

et al. 2022

Front. Pharmacol.

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Depression is a common mental disorder characterized by pessimism and world-weariness. In our previous study, we found that Xiaoyaosan (XYS) could have antidepressive effects, however the underlying mechanisms remain unclear. Several studies have shown that adenosine A (2 A) receptor (A2AR) in the brain is a key point in the treatment of depression. Our present study aimed to investigate the effects of XYS on A2AR signaling in the striatum of rats exposed to chronic restraint stress (CRS). Ninety-six male Sprague–Dawley rats were randomly divided into 8 groups (control, model, negative control, XYS, A2AR antagonist, A2AR antagonist + XYS, A2AR agonist, A2AR agonist + XYS). The rats in the model group, XYS group, A2AR antagonist group and A2AR antagonist + XYS group were subjected to CRS for 3 h a day. The XYS decoction [2.224 g/(kg·d)] was intragastrical administered by oral gavage to the rats in the negative control group, XYS group, A2AR antagonist + XYS group, and A2AR agonist + XYS group. The rats in the A2AR antagonist group and A2AR antagonist + XYS group were treated with SCH 58261 [0.05 mg/(kg·d)], and the rats in the A2AR agonist and A2AR agonist + XYS group were treated with CGS 21680 [0.1 mg/(kg·d)]. These procedures were performed for 21 consecutive days. Behavioral studies including the open field test, elevated plus maze test, sucrose preference test and forced swimming test, were performed to examine depression-like phenotypes. Then, the effects of XYS on CRS- or A2AR agonist-induced striatal subcellular damage, microglial activation and A2AR signaling changes in the striatum were examined. Here, we report that XYS ameliorates depression-like phenotypes (such as body weight loss as well as depression- and anxiety-like behaviors) and improves synaptic survival and growth in the stratum of the CRS rats. Moreover, XYS reduces A2AR activity and suppresses hyper-activation of striatal microglia. The tissue and cellular effects of XYS were similar to those of the known A2AR antagonists. In conclusion, XYS alleviates depression in the CRS rats via inhibiting A2AR in the striatum.

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Section: Discussionmentioning

confidence: 99%

Xiaoyaosan Ameliorates Chronic Restraint Stress-Induced Depression-Like Phenotype by Suppressing A2AR Signaling in the Rat Striatum

Zhu

Yang

et al. 2022

Front. Pharmacol.

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“…High performance liquid chromatography (HpLc). HPLC system used in the present study was based in previous studies [33][34][35][36] using a Shimadzu (LC-10 AD) model with an injection circuit accomplished to LC-20AT pump, analytical Shimadzu C18 column and fluorescence detector (RF-10AXL). The mobile phase (A phase) was composed by 50 mM sodium acetate, 5% methanol and 2-propanol.…”

Section: Drugs and Reagentsmentioning

confidence: 99%

Acute Restraint Stress Evokes Anxiety-Like Behavior Mediated by Telencephalic Inactivation and GabAergic Dysfunction in Zebrafish Brains

Assad

Luz

Santos-Silva

et al. 2020

Sci Rep

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Acute stress is an important factor in the development of anxiety disorders. Zebrafish are an organism model widely used by studies that aim to describe the events in the brain that control stress-elicited anxiety. the goal of the current study was to evaluate the pattern of cell activation in the telencephalon of adult zebrafish and the role of the GABAergic system on the modulation of anxiety-like behavior evoked by acute restraint stress. Zebrafish that underwent acute restraint stress presented decreased expression of the c-fos protein in their telencephalon as well as a significant decrease in GABA release. The data also supports that decreased GABA levels in zebrafish brains have diminished the activation of GABAA receptors eliciting anxiety-like behavior. Taken together these findings have helped clarify a neurochemical pathway controlling anxiety-like behavior evoked by acute stress in zebrafish while also opening the possibility of new perspective opportunities to use zebrafish as an animal model to test anxyolitic drugs that target the GABAergic system. open Scientific RepoRtS | (2020) 10:5551 | https://doi.

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Cochlear glial cells mediate glutamate uptake through a sodium-independent transporter

Martins¹,

Silva²,

Pinheiro³

et al. 2023

Hearing Research

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Adenosine A1 receptors modulate the Na+-Hypertonicity induced glutamate release in hypothalamic glial cells

Cited by 3 publications

References 31 publications

Xiaoyaosan Ameliorates Chronic Restraint Stress-Induced Depression-Like Phenotype by Suppressing A2AR Signaling in the Rat Striatum

Xiaoyaosan Ameliorates Chronic Restraint Stress-Induced Depression-Like Phenotype by Suppressing A2AR Signaling in the Rat Striatum

Acute Restraint Stress Evokes Anxiety-Like Behavior Mediated by Telencephalic Inactivation and GabAergic Dysfunction in Zebrafish Brains

Cochlear glial cells mediate glutamate uptake through a sodium-independent transporter

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