Adenocarcinoma of the lung: Electron microscopy of fine-needle aspiration biopsy specimens?a review of 73 cases

Reyes, Cesar V.; Jensen, JoAnne; Graham, Galen

doi:10.1002/(sici)1097-0339(199905)20:5<257::aid-dc2>3.0.co;2-e

Cited by 3 publications

(1 citation statement)

References 7 publications

(15 reference statements)

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“…According to the histological heterogeneity found in clinical, imaging, molecular biological, and pathological studies, lung adenocarcinoma is divided into four subtypes: preinvasive lesions, minimally invasive adenocarcinoma, invasive adenocarcinoma, and variants of invasive adenocarcinoma ( 1 ). Lung adenocarcinoma cells can also be subclassified into six cytological subtypes by electron microscopy: bronchial cell surface type with little or no mucus, goblet cell type, bronchial gland cell type, Clara cell type, type II alveolar epithelial cell type, and mixed cell type ( 2 - 4 ).…”

Section: Introductionmentioning

confidence: 99%

Proteomic and ultrastructural analysis of Clara cell and type II alveolar epithelial cell-type lung cancer cells

Hou¹,

Chang²,

Liu³

et al. 2020

Transl Cancer Res TCR

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Background: Currently, the identification of Clara cell and type II alveolar epithelial cell-type cancer cells requires electron microscopy, which is a time-consuming and expensive process involving a complicated tissue sampling procedure. The aim of this study was to identify unique biomarkers for Clara cell and type II alveolar epithelial cell-type lung cancer cells, respectively, with proteomic profiling.Methods: Six human lung adenocarcinoma cell lines (A549, NCI-H358, NCI-H1650, HCC827, NCI-H1395, and NCI-H1975) were investigated for their ultrastructural characteristics. The differentially expressed proteins (DEPs) were screened between NCI-H358 cells (Clara cell type) and A549 cells (type II alveolar epithelial cell type) using two-dimensional difference gel electrophoresis (2D-DIGE) and matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS), and then they were validated by western blot. The protein expression levels of endoplasmic reticulum oxidoreductin 1-α (ERO1L), Clara cell 10-kD protein (CC10), and surfactant protein C (SP-C) were also determined in the six cell lines assayed.Results: NCI-H358 cells featured Clara cell differentiation; A549, NCI-H1975, and HCC827 cells had characteristics of type II alveolar epithelial cells; and NCI-H1395 and NCI-H1650 cells had no differentiation characteristics of any lung adenocarcinoma cell type. Five DEPs including ubiquitin carboxylterminal hydrolase isozyme L1 (UCHL1), cytokeratin 19 (CK19), cytokeratin 8 (CK8), ERO1L, and peroxiredoxin 2 (PRDX2) between NCI-H358 and A549 cells were identified for further validation; however, none of them showed suitability as an effective biomarker. Similarly, CC10 and SP-C were not appropriate biomarkers.Conclusions: Cytological subtypes of NCI-H1975 and HCC827 cells were identified, but no promising biomarker was discovered in the present study.

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Section: Introductionmentioning

confidence: 99%

Proteomic and ultrastructural analysis of Clara cell and type II alveolar epithelial cell-type lung cancer cells

Hou¹,

Chang²,

Liu³

et al. 2020

Transl Cancer Res TCR

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Lung, chest wall and pleura

Segal¹,

Frost²,

Silverman³

2012

Orell and Sterrett's Fine Needle Aspiration Cytology

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Pulmonary Carcinomas With Mucinous and Neuroendocrine Differentiation

Weissferdt

2018

American Journal of Surgical Pathology

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Amphicrine carcinoma is a distinct type of carcinoma characterized by synchronous exocrine and endocrine differentiation within the same tumor cell. Such tumors are exceedingly rare and most commonly recognized in the gastrointestinal tract. In the lung, sporadic descriptions of such lesions exist. This report presents 3 more such tumors, expanding the spectrum of amphicrine carcinomas in this organ. The patients were 3 men, 53 to 78 years of age (mean: 63 y) and all were current or former smokers. In one patient the tumor was an incidental finding, the others presented with lower respiratory symptoms. Microscopically, the lesions had an organoid pattern, consisting of nests of large tumor cells with peripheral palisading, vesicular nuclei and prominent nucleoli reminiscent of large cell neuroendocrine carcinoma. In addition, the tumor nests also showed glandular features, characterized by gland-like lumina filled with mucin and intracytoplasmic mucin droplets. Immunohistochemical studies showed diffuse reactivity of the tumor cells with synaptophysin and chromogranin and variable reactivity with TTF-1. Mucin stains highlighted extracellular and intracellular mucin deposition. Clinical follow-up demonstrated that one patient had died of his disease 12 months after surgery while another was alive and free of disease 21 months after surgery. The third patient was alive with widespread metastatic disease 8 months after diagnosis. This series highlights a rare variant of pulmonary carcinoma showing synchronous exocrine and endocrine differentiation. Recognition of this type of tumor is important in terms of tumor classification and to identify an entity with potential aggressive behavior.

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Adenocarcinoma of the lung: Electron microscopy of fine-needle aspiration biopsy specimens?a review of 73 cases

Cited by 3 publications

References 7 publications

Proteomic and ultrastructural analysis of Clara cell and type II alveolar epithelial cell-type lung cancer cells

Proteomic and ultrastructural analysis of Clara cell and type II alveolar epithelial cell-type lung cancer cells

Lung, chest wall and pleura

Pulmonary Carcinomas With Mucinous and Neuroendocrine Differentiation

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