Adeno-associated virus packaged TRPC5 gene therapy alleviated spinal cord ischemic reperfusion injury in rats

Abstract

Spinal cord injury (SCI) is a devastating disease with few effective treatments. This study mainly explored the mechanism of TRPC5 gene in the treatment of spinal cord ischemia reperfusion injury from the perspective of angiogenesis. Western blot, immunohistochemistry, hematoxylin and eosin, ELISA, and reverse transcription-PCR (RT-PCR) were used to detect the expression levels of related angiogenic proteins such as von Willebrend factor (vWF), vascular endothelial growth factor (VEGF), CD31, and HIF-1α. The results showed that compared with the IR group, the Basso, Beattie, and Bresnahan scores of IR + adeno-associated virus (AAV) + TRPC5 group were higher with significant difference. And compared with ischemia/reperfusion (I/R) group, RT-PCR and ELISA results showed that inflammatory factors such as IL-6, IL-1β, and TNF-α were significantly reduced in IR + AAV + TRPC5 group. In addition, the expression of vascular related proteins such as vWF, VEGF, and CD31 in spinal cord tissue were all increased. Taken together the results, we suggest that TRPC5 could significantly increase the expression of angiogenic protein and slow down the occurrence of inflammatory response to repair the SCI.

Drug Discovery in Spinal Cord Injury With Ankylosing Spondylitis Identified by Text Mining and Biomedical Databases

“…Experiencing compressive injury, the levels of VWF and VEGFA expression in the rat spinal cord were decreased. Interestingly, the levels of two gene expressions were dramatically increased after decompression (Cheng et al, 2021), which was consensus with another study (Shen et al, 2020). Furthermore, VWF production in men with AS was more than Each drug-gene interaction ensured that the hypothetical drug had an expected effect on the condition.…”

Wirelessly Powered Electrical-Stimulation Based on Biodegradable 3D Piezoelectric Scaffolds Promotes the Spinal Cord Injury Repair

“…Angiogenesis is another important guarantee for the repair of the injured spinal cord . In the quantitative results of immunofluorescent staining at week 8 postsurgery, there are abundant platelet endothelial cell adhesion molecule-1 and vascular endothelial growth factor (CD31/VEGF) labeled vascular endothelial cells in the PLA/KNN@PDA-US groups, which are much more numerous than those in the other control groups (Figure G–I).…”

“…Angiogenesis is another important guarantee for the repair of the injured spinal cord. 50 In the quantitative results of immunofluorescent staining at week 8 postsurgery, there are abundant platelet endothelial cell adhesion molecule-1 and vascular endothelial growth factor (CD31/VEGF) labeled vascular endothelial cells in the PLA/KNN@PDA-US groups, which are much more numerous than those in the other control groups (Figure 7G−I). The results indicated that the US-driven wireless ES based on PLA/KNN@PDA piezoelectric scaffolds enhances the expression of the vascular endothelial growth factor and accelerates angiogenesis and therefore provides an excellent platform for tissue regeneration.…”

New Insights into the Reparative Angiogenesis after Myocardial Infarction

“…Similarly, TRPC5 channel has been proposed as fair therapeutic candidate that may recover ischemic tissue through angiogenesis [87], because TRPC5 expression together with Orai1 was increased after MI, forming SOC channels [6]. TRPC5 role in angiogenesis has been proved in hind limb ischemia model [88], and an animal model of spinal cord ischemia/reperfusion (I/R) injury [89]. Nevertheless, the impact of TRPC5 on the improvement of myocardial angiogenesis has not been demonstrated.…”