Adeno-Associated Virus-Mediated Single-Cell Labeling of Mitral Cells in the Mouse Olfactory Bulb: Insights into the Developmental Dynamics of Dendrite Remodeling

Togashi, Katsumi; Tsuji, Michiko; Takeuchi, Shunsuke; Nakahama, Ryota; Koizumi, Hiroyuki; Emoto, Kazuo

doi:10.3389/fncel.2020.572256

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…After extending multiple processes into different glomeruli, M/TC apical dendrites undergo an extensive process of morphological refinement between postnatal day (P) 0 and P7. By around P6, the vast majority of M/TCs have an extensively branched apical tuft in just one glomerulus ( Blanchart et al, 2006 ; Fujimoto et al, 2019 preprint; Malun and Brunjes, 1996 ; Santacana et al, 1992 ; Togashi et al, 2020 ) ( Fig. 2 ).…”

Section: Ob Glutamatergic Neuron Developmentmentioning

confidence: 99%

Development of the mammalian main olfactory bulb

et al. 2022

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The mammalian main olfactory bulb is a crucial processing centre for the sense of smell. The olfactory bulb forms early during development and is functional from birth. However, the olfactory system continues to mature and change throughout life as a target of constitutive adult neurogenesis. Our Review synthesises current knowledge of prenatal, postnatal and adult olfactory bulb development, focusing on the maturation, morphology, functions and interactions of its diverse constituent glutamatergic and GABAergic cell types. We highlight not only the great advances in the understanding of olfactory bulb development made in recent years, but also the gaps in our present knowledge that most urgently require addressing.

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Section: Ob Glutamatergic Neuron Developmentmentioning

confidence: 99%

Development of the mammalian main olfactory bulb

et al. 2022

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“…Adeno-associated virus injection. We performed AAVs injection as previously described 52,53 . In brief, mice 6-8 weeks of age were stereotaxically injected.…”

Section: Methodsmentioning

confidence: 99%

The circadian clock in the piriform cortex intrinsically tunes daily changes of odor-evoked neural activity

et al. 2023

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The daily activity in the brain is typically fine-tuned by the circadian clock in the local neurons as well as by the master circadian clock in the suprachiasmatic nucleus (SCN) of the hypothalamus. In the olfactory response, odor-evoked activity in the piriform cortex (PC) and olfactory behavior retain circadian rhythmicity in the absence of the SCN, yet how the circadian rhythm in the PC is achieved independently of the SCN remains elusive. Here, to define neurons regulating the circadian rhythm of the odor-evoked activity in the PC, we knocked out the clock gene Bmal1 in a host of specific neurons along the olfactory circuit. We discovered that Bmal1 knockout in the PC largely abolishes the circadian rhythm of the odor-evoked activity. We further showed that isolated PC exhibits sustained circadian rhythms of the clock gene Per2 expression. Quantitative PCR analysis revealed that expression patterns of multiple genes involved in neural activity and synaptic transmission exhibit circadian rhythm in the PC in a BMAL1-dependent manner. Our findings indicate that BMAL1 acts intrinsically in the PC to control the circadian rhythm of the odor-evoked activity in the PC, possibly through regulating expression patterns of multiple genes involved in neural activity and transmission.

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Deficiency of CAMSAP2 impairs olfaction and the morphogenesis of mitral cells

Zhou,

Yang,

Mao

et al. 2024

EMBO Rep

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In developing olfactory bulb (OB), mitral cells (MCs) remodel their dendrites to establish the precise olfactory circuit, and these circuits are critical for individuals to sense odors and elicit behaviors for survival. However, how microtubules (MTs) participate in the process of dendritic remodeling remains elusive. Here, we reveal that calmodulin-regulated spectrin-associated proteins (CAMSAPs), a family of proteins that bind to the minus-end of the noncentrosomal MTs, play a crucial part in the development of MC dendrites. We observed that Camsap2 knockout (KO) males are infertile while the reproductive tract is normal. Further study showed that the infertility was due to the severe defects of mating behavior in male mice. Besides, mice with loss-of-function displayed defects in the sense of smell. Furthermore, we found that the deficiency of CAMSAP2 impairs the classical morphology of MCs, and the CAMSAP2-dependent dendritic remodeling process is responsible for this defect. Thus, our findings demonstrate that CAMSAP2 plays a vital role in regulating the development of MCs.

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Adeno-Associated Virus-Mediated Single-Cell Labeling of Mitral Cells in the Mouse Olfactory Bulb: Insights into the Developmental Dynamics of Dendrite Remodeling

Cited by 4 publications

References 42 publications

Development of the mammalian main olfactory bulb

Development of the mammalian main olfactory bulb

The circadian clock in the piriform cortex intrinsically tunes daily changes of odor-evoked neural activity

Deficiency of CAMSAP2 impairs olfaction and the morphogenesis of mitral cells

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