Adeno-Associated Virus-Mediated MicroRNA Delivery and Therapeutics

Xie, Jun; Burt, Daniel Robert; Gao, Guangping

doi:10.1055/s-0034-1397352

Cited by 27 publications

(19 citation statements)

References 80 publications

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“…Baculovirus-mediated miR delivery offers unique advantages because baculovirus cannot proliferate in mammalian hosts and because baculovirus degrades in the host over time, making this methodology a promising therapeutic approach [ 84 ]. Presently, AAV is the most encouraging viral system for gene therapy, as it can prolong transgene expression in its host and can transduce dividing and non-dividing cells [ 84 , 87 ]. AAV is non-pathogenic and thus poses a minimal threat to immune systems in humans.…”

Section: Strategies For Therapeutic Deliverymentioning

confidence: 99%

The Role of Hypoxia-Induced miR-210 in Cancer Progression

Dang

Myers

2015

IJMS

142

117

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Prolonged hypoxia, the event of insufficient oxygen, is known to upregulate tumor development and growth by promoting the formation of a neoplastic environment. The recent discovery that a subset of cellular microRNAs (miRs) are upregulated during hypoxia, where they function to promote tumor development, highlights the importance of hypoxia-induced miRs as targets for continued investigation. miRs are short, non-coding transcripts involved in gene expression and regulation. Under hypoxic conditions, miR-210 becomes highly upregulated in response to hypoxia inducing factors (HIFs). HIF-1α drives miR-210’s overexpression and the resultant alteration of cellular processes, including cell cycle regulation, mitochondria function, apoptosis, angiogenesis and metastasis. Here we discuss hypoxia-induced dysregulation of miR-210 and the resultant changes in miR-210 protein targets that regulate cancer progression. Potential methods of targeting miR-210 as a therapeutic tool are also explored.

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Section: Strategies For Therapeutic Deliverymentioning

confidence: 99%

The Role of Hypoxia-Induced miR-210 in Cancer Progression

Dang

Myers

2015

IJMS

142

117

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“…Although the adenovirus-mediated delivery of TNA seems straightforward, there are a number of fundamental limitations such as the size of the construct for expression and the existence of an adaptive immune response by B cells or CD8 T cells in a significant number of people. 154 Therefore, downregulation of ncRNA in the liver is preferable by the use of chemically modified TNA and alternative nonviral delivery systems. The liver is the main target organ for systemic delivery of modified TNA due to efficient mechanisms of receptor-mediated delivery.…”

Section: Targeting Mirna and Lncrna In The Livermentioning

confidence: 99%

Noncoding RNA in Liver Regeneration—From Molecular Mechanisms to Clinical Implications

2019

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The unique ability of the adult liver to regenerate after injury is the basis for efficient surgical resection and liver transplantation and provides solutions for the treatment of liver cancer and acute liver failure. Current success in surgical treatments could be enhanced by directed regulation of liver regeneration. A number of small molecules and growth factors have been tested in mice models to improve liver regeneration. Noncoding ribonucleic acids (ncRNA) are less studied regulators of various cellular processes. Here, the authors carefully review ncRNA involved in liver regeneration and discuss molecular mechanisms and regulatory networks. These ncRNAs modulate the expression of pro- and antiproliferative genes allowing to orchestrate precisely the proliferation of hepatocytes. The authors expect that ncRNA will become new targets in liver regeneration due to recent progress in therapeutic nucleic acids. Among a large number of preclinical studies on ncRNA, only a few entered clinical trials, and further studies are needed to uncover their potential as therapeutic targets.

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“…Viral vectors have been utilised in order to deliver miR mimics or inhibitors. For example, adeno-associated virus (AAV) is a popular gene delivery system; however, a consistent issue which has inhibited the progression of some AAV therapies to human clinical trials is the induction of low grade immune activation (118). Lenti-viral vectors for delivery suffer from the same immunogenicity issues.…”

Section: Microrna Therapeuticsmentioning

confidence: 99%

MicroRNAs in the skin: role in development, homoeostasis and regeneration

et al. 2017

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The skin is the largest organ of the integumentary system and possesses a vast number of functions. Due to the distinct layers of the skin and the variety of cells which populate each, a tightly regulated network of molecular signals control development and regeneration, whether due to programmed cell termination or injury. MicroRNAs (miRs) are a relatively recent discovery; they are a class of small non-coding RNAs which possess a multitude of biological functions due to their ability to regulate gene expression via post-transcriptional gene silencing. Of interest, is that a plethora of data demonstrates that a number of miRs are highly expressed within the skin, and are evidently key regulators of numerous vital processes to maintain non-aberrant functioning. Recently, miRs have been targeted as therapeutic interventions due to the ability of synthetic 'antagomiRs' to down-regulate abnormal miR expression, thereby potentiating wound healing and attenuating fibrotic processes which can contribute to disease such as systemic sclerosis (SSc). This review will provide an introduction to the structure and function of the skin and miR biogenesis, before summarizing the literature pertaining to the role of miRs. Finally, miR therapies will also be discussed, highlighting important future areas of research.

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Adeno-Associated Virus-Mediated MicroRNA Delivery and Therapeutics

Cited by 27 publications

References 80 publications

The Role of Hypoxia-Induced miR-210 in Cancer Progression

The Role of Hypoxia-Induced miR-210 in Cancer Progression

Noncoding RNA in Liver Regeneration—From Molecular Mechanisms to Clinical Implications

MicroRNAs in the skin: role in development, homoeostasis and regeneration

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