Adenine sulphate and AgNO3PromoteIn vitroShoot Proliferation inMillettia pinnata(L.) Panigrahi-a Biodiesel Producing Tree

Nagar, Durga Singh; Jha, Suman Kumar; Jani, Jigar N.; Chauhan, R. S.

doi:10.5958/2229-4473.2015.00031.2

Cited by 2 publications

(1 citation statement)

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“…However, BAP was able to induce higher multiple shoot bud frequency compared to TDZ. In previous studies, multiple shoot buds or multiple shoots of P. pinnata were induced from nodal segments (Kesari et al 2012) and cotyledonary nodes (Nagar et al 2015a(Nagar et al , 2015b using BAP. Besides BAP, TDZ was also able to induce multiple shoot buds or multiple shoots from different explants such as deembryonated cotyledon (Sujatha et al 2008), cotyledonary node (Belide et al 2010), axillary meristem (Sujatha and Hastra 2006;Sujatha and Hazra 2007), embryo and hypocotyl explants (Humánez et al 2011;Sharma et al 2011).…”

Section: Multiple Shoot Bud Induction From Nodal Explantsmentioning

confidence: 99%

Multiple shoot bud induction and plant regeneration studies of Pongamia pinnata

Tan

Tee

Wong

2018

Plant Biotechnology

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Pongamia pinnata is a legume plant which has great potential to be used as a biofuel feedstock. Conventional propagation of P. pinnata was found to be inefficient for mass propagation. Employing plant tissue culture techniques for micropropagation and further plant improvement of P. pinnata will be the right path to fulfill future challenges in biofuel production. This study aimed to establish a plant regeneration system for potential micropropagation and genetic manipulation of P. pinnata in future. In vitro nodal explants were used and Woody Plant Medium (WPM) containing 30 µM 6-benzylaminopurine (BAP) and 1 mM phloroglucinol (PG) was able to induce higher frequency of multiple shoot buds compared to other media investigated in this study. For shoot regeneration study, WPM containing 15 µM of zeatin and 1 mM PG was able to induce longer shoots while rooting of the regenerated shoots was enhanced by WPM supplemented with indole-3-butyric acid (IBA) in combination with silver thiosulphate (STS). A simple and effective acclimatisation protocol was established with very high survival frequency of regenerated plantlets. Root nodulation of the successfully acclimatised plants was also observed. In short, multiple shoot buds were successfully induced, regenerated and rooted in vitro. The rooted plantlets were successfully acclimatised and grown healthily. It was concluded that a successful plant regeneration protocol of P. pinnata was achieved for potential application in micropropagation and genetic manipulation.

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Section: Multiple Shoot Bud Induction From Nodal Explantsmentioning

confidence: 99%

Multiple shoot bud induction and plant regeneration studies of Pongamia pinnata

Tan

Tee

Wong

2018

Plant Biotechnology

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Direct regeneration and Genetic transformation studies in Hemidesmus indicus (L.) R. Br. (Indian Sarasaprilla)

Ranganatha

Sharma

et al. 2023

Preprint

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Since ages, plants continue to provide new remedies to mankind. Hemidesmus indicus L. R. Br. is one such plant belonging to family Apocynaceae, showing potent medicinal properties known through traditional knowledge. Hemidesmus is also explored for the presence of flavoring compound namely 2-hydroxy-4-methoxybenzaldehyde (HMB) which is used in pharmaceutical and nutraceutical industries. Due to anthropogenic activities, the plant has been exploited till the ridge for its ethnobotanical properties for mankind. Biotechnological intervention to conserve this endangered sps through in vitro plant cultures, micropropogation and genetic transformation studies is the pre-requite to maintain it from extinction. The objective of the study is to improve the regeneration potential and optimize the genetic transformation in Hemidesmus indicus. The direct regeneration of Hemidesmus indicus through leaf explants, nodal explants with subsequent plant regeneration using Murashige and Skoog (MS) medium supplemented with various plant growth regulators (auxins, cytokinins, and gibberellic acid), adenine sulphate, TRIA. The Agrobacterium tumefaciens mediated genetic transformation studies in Hemidesmus indicus was carried out in callus cultures using the plant expression vector pCAMBIA 1301. The caulogenic response of 78.8%, 73.3% and 71.4% was observed when the leaf explant was inoculated on MS media containing 2.3 mgL− 1 BAP + 0.2 mgL− 1 2,4-D, 0.02 mgL− 1 TRIA + 2 mgL− 1 BAP, 1 mgL− 1 KIN + 1 mgL− 1 NAA respectively with creamish yellow nodular friable callus by the 4 weeks. The initiation of shoot bud was observed within three days after inoculation of nodal explant on media supplemented with 1 mgL− 1 BAP + 0.1 mgL− 1 NAA, 1 mgL− 1 BAP + 0.1 mgL− 1 NAA + 40 mgL− 1 AgNO3, 1 mgL− 1 BAP + 0.1 mgL− 1 NAA + 40 mgL− 1 AgNO3 + 40 mgL− 1 adenine sulphate respectively and incubated in the dark for 2 weeks. Shoot regeneration from the leaf explants was also observed within 4 weeks after inoculation in MS medium with 1 mgL− 1 BAP + 0.1 mgL− 1 NAA. Agrobacterium mediated genetic transformation was carried out successfully in callus cultures of H. indicus. The transformation efficiency was found to be 26%. The efficient shoot regeneration was observed within 4 weeks and transformation study can be further applied for over expression of biosynthetic genes to enhance the bioactive components that have immense significance in pharmaceutical, nutraceutical and cosmetic industries.

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Adenine sulphate and AgNO₃PromoteIn vitroShoot Proliferation inMillettia pinnata(L.) Panigrahi-a Biodiesel Producing Tree

Cited by 2 publications

References 0 publications

Multiple shoot bud induction and plant regeneration studies of <i>Pongamia pinnata</i>

Multiple shoot bud induction and plant regeneration studies of <i>Pongamia pinnata</i>

Direct regeneration and Genetic transformation studies in Hemidesmus indicus (L.) R. Br. (Indian Sarasaprilla)

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