Adenine Nucleotides of Ischemic Intestine Do Not Reflect Injury

Canada, Andrew T.; Coleman, Larry; Fabian, Michael; Bollinger, R. Randal

doi:10.1006/jsre.1993.1162

Cited by 11 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They were also comparable to results from the rat heart [8,18], small intestine [11][12], and blood vessels ment of their values for indicating ischemia cannot be made. However, the data from Soussi et al, [24] indi- [19][20].…”

Section: Discussionsupporting

confidence: 86%

“…Canada et cleotides to reflect a high oxygenation and oxygen conal. [12] have suggested that recovery of small intestine sumption state induced by skeletal muscle, muscle mefrom ischemia is not well predicted from ATP levels. tabolites during hyperoxemia induced by high perfuIn comparison, xanthine did seem to correlate with sion flow rate plus norepinephrine infusion [6] were ischemia.…”

Section: Discussionmentioning

confidence: 98%

“…In addition, lactate and glycerol were enzymatically measured to assess potential de novo synthesis of the energy compounds from glycolysis and lipolysis of triglyceride during the process. In the heart and intestine, ATP levels are particularly sensitive to oxygen supply and immediately falls when blood flow to the tissues decreases [11][12][13]. However, the use of adenine nucleotides as indicators for early ischemia or hypoxia in tissues such as skeletal muscle [14] and intestine [11] are not certain.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Creatine Phosphate as the Preferred Early Indicator of Ischemia in Muscular Tissues

Clark

Colquhoun

1996

Journal of Surgical Research

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Creatine Phosphate as the Preferred Early Indicator of Ischemia in Muscular Tissues

Clark

Colquhoun

1996

Journal of Surgical Research

View full text Add to dashboard Cite

“…Analogously, EC values of human uterine and bladder smooth muscle were distinctly lower than those of rectus striated muscle [25,26], in agreement with those obtained on intestinal wall biopsies in the present study. Our present energy data on pig intestinal wall biopsies are also concordant with previous data on rat intestinal wall biopsies [27][28][29]. This means that an EC value of about 0.82 should be considered as physiological for smooth muscle although different from the expected normal EC value of the rectus striated muscle of about 0.92.…”

Section: Discussionsupporting

confidence: 81%

Intramucosal pH and pCO<sub>2</sub> Do Not Strictly Correlate with Intestinal Energy Metabolism in Experimental Peritonitis

Ljungdahl

Rasmussen²,

Ronquist³

et al. 2000

Eur Surg Res

View full text Add to dashboard Cite

This study aimed to investigate tissue hypoxia on the cellular level in sepsis. Eighteen pigs weighing 18–27 kg were studied. Intramucosal-arterial PCO₂ gradient (PCO₂-gap) and intramucosal pH (pH_i) were calculated using tonometry. A blind loop of the small intestine was constructed for repeated tissue biopsies to measure intestinal energy-related metabolites and lactate concentration. Six animals served as controls. In 12 animals, faecal peritonitis was induced. Six of these animals were studied without further interventions, while the others were resuscitated with dextran to maintain cardiac index at baseline level. Untreated peritonitis caused an increase in PCO₂-gap and a drop in pH_i. The intestinal energy metabolism was not disturbed until the end of the experimental period, with a decreased energy charge value and a moderately increased lactate concentration. In peritonitis-dextran animals, PCO₂-gap and pH_i remained at baseline level and the energy metabolism was not disturbed. We conclude that in peritonitis, PCO₂-gap – like pH_i – can be influenced by other factors than strictly anaerobic tissue metabolism.

show abstract

“…These biochemical findings did not correlate with the more severe histologic mucosal damage seen in the small intestine during ischemia and after reperfusion. Indeed, Canada et al [25] indicated that xanthine was the only biochemical marker that correlated with the duration of ischemia, but energy charge was of no value in indicating the extent of injury in the small intestine. Hypothermic storage significantly retarded adenine nucleotide and purine catabolite changes.…”

Section: Discussionmentioning

confidence: 99%

Effect of Ischemia on the Canine Large Bowel: A Comparison with the Small Intestine

Takeyoshi

Zhang

Nakamura

et al. 1996

Journal of Surgical Research

View full text Add to dashboard Cite

Mucosal injury caused by ischemia and reperfusion has been well documented with the small intestine, but little is known about the colon. In the present study, the effect of warm and cold ischemia on the canine colon was studied and compared to that on the small intestine. After in situ flushing, the small intestine and the colon from six beagle dogs were removed and stored for 0.5, 1.5, and 3 hr at 37°C (warm ischemia) or for 1, 6, 12, 24, 36, and 48 hr at 4°C (cold ischemia). Electrophysiology, permeability, biochemistry, and histopathology of the specimens at each ischemic period and after reperfusion in the Ussing chamber were determined. Warm and cold ischemia induced durationdependent suppression of electrophysiology in both organs, but the colonic mucosa retained higher activity of absorptive enterocytes and cryptic cells than the small intestine. Only the colon showed increased permeability of FITC-conjugated Dextran from the mucosal surface to the submucosal layer after prolonged ischemia. Changes in adenine nucleotides and purine catabolites were not markedly different between the organs. Histopathologic abnormalities during ischemia and after reperfusion were more serious with the small intestine than with the colon. Compared to warm ischemia, hypothermia lessened or delayed these morphofunctional derangements in both organs, which became universally worsened after reperfusion. Colonic mucosa receives morphofunctional derangements from ischemia and reperfusion, but the severity of the damage was much less severe in the colon than in the small intestine.

show abstract

Adenine Nucleotides of Ischemic Intestine Do Not Reflect Injury

Cited by 11 publications

References 0 publications

Creatine Phosphate as the Preferred Early Indicator of Ischemia in Muscular Tissues

Creatine Phosphate as the Preferred Early Indicator of Ischemia in Muscular Tissues

Intramucosal pH and pCO<sub>2</sub> Do Not Strictly Correlate with Intestinal Energy Metabolism in Experimental Peritonitis

Effect of Ischemia on the Canine Large Bowel: A Comparison with the Small Intestine

Contact Info

Product

Resources

About