1969
DOI: 10.1016/0005-2787(69)90003-3
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Adenine nucleotide metabolism of blood platelets VI. Subcellular localization of nucleotide pools with different functions in the platelet release reaction

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Cited by 160 publications
(54 citation statements)
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“…Note that the granule fractions also contain metabolic ATP and ADP localized in the mitochondria (less than 3% of total metabolic ATP and ADP, Ref. 19). …”
Section: Analysis Of Metabolic Parametersmentioning
confidence: 99%
“…Note that the granule fractions also contain metabolic ATP and ADP localized in the mitochondria (less than 3% of total metabolic ATP and ADP, Ref. 19). …”
Section: Analysis Of Metabolic Parametersmentioning
confidence: 99%
“…At least 80-90% of release has occurred by 15 sec. By' 30 sec, the total amount of releasable nucleotides and calcium, amounting to approximately 60% of the platelet's total content of these substances (20), appears in the extracellular medium. Incubation to 5 min does not change these levels.…”
Section: Cmentioning
confidence: 99%
“…The tubes were centrifuged at 100 g for 12 min and the platelet-rich supernatant was kept at room tempera- The aggregating agents were adrenaline (1 liM), ADP (1 or 2 pM), and 0.01 ml of the collagen preparation of Holmsen, Day & Storm (1969). They were added to the platelet-rich plasma to give a final volume of 0.4 ml.…”
Section: Methodsmentioning
confidence: 99%