AdEasy-based cloning system to generate tropism expanded replicating adenoviruses expressing transgenes late in the viral life cycle

Rapid, Efficient, and Modular Generation of Adenoviral Vectors via Isothermal Assembly

“…This technology, called the pAdEasy system, is commercially available (Murakami et al, 2002). It is efficient in recombination; however, it is complex and requires a three-step transformation in two types of E. coli (Zeng et al, 2001;Lie et al, 2005;Luo et al, 2007;Janssen et al, 2013).…”

Development of an AdEasy‐based system to produce first‐ and second‐generation adenoviral vectors with tropism for CAR‐ or CD46‐positive cells

“…However, it was found that the resulting SG AdV preparations displayed ten‐fold lower titers than those corresponding to their E4 ‐positive counterparts. In addition, although the AdEasy system has been adapted to generate AdVs with tropism‐altering fiber modifications , these endeavors were, to the best of our knowledge, never tested in the context of SG AdVs. In the present study, we report the construction and validation of an AdEasy‐based system for the efficient generation of E1 ‐ and E1 ‐ plus E2A ‐deleted AdVs equipped with the prototypic HAdV5 fiber (F5) or with a chimeric fiber consisting of the tail of HAdV5 and the shaft and knob motifs of HAdV serotype 50 (F5/50).…”

Efficacy of recombinant adenovirus-mediated double suicide gene therapy in human keloid fibroblasts

“…Additionally, high titres (amplified up to 10 11 plaque-forming units (pfu) ⁄ mL) and high-level gene expression can be obtained. 7 Members of the Bcl-2 family are regulators of apoptotic signalling, and have been shown to be dysregulated in diverse pathological conditions associated with apoptosis. 8 Among the Bcl-2 family members, Bcl-2, an antiapoptotic gene, and BAX, a pro-apoptotic gene, play important roles in the process of apoptosis.…”