Addressing the recent outbreak of African horse sickness in Lagos, Nigeria

Adesola, Ridwan Olamilekan; Bakre, Adetolase; Gulumbe, Bashar Haruna

doi:10.3389/fvets.2023.1160856

Cited by 1 publication

(1 citation statement)

References 7 publications

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“…In December 2022, an AHS outbreak was notified in Nigeria [ 41 , 42 ], a country where AHS is endemic. Ten clinical samples were received by the LCV, and AHSV serotype 9 was detected in all of them by TS rRT-PCR typing assays.…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications

Villalba,

Tena-Tomás,

Ruano

et al. 2024

Viruses

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The African horse sickness virus (AHSV) belongs to the Genus Orbivirus, family Sedoreoviridae, and nine serotypes of the virus have been described to date. The AHSV genome is composed of ten linear segments of double-stranded (ds) RNA, numbered in decreasing size order (Seg-1 to Seg-10). Genome segment 2 (Seg-2) encodes outer-capsid protein VP2, the most variable AHSV protein and the primary target for neutralizing antibodies. Consequently, Seg-2 determines the identity of the virus serotype. An African horse sickness (AHS) outbreak in an AHS-free status country requires identifying the serotype as soon as possible to implement a serotype-specific vaccination program. Considering that nowadays ‘polyvalent live attenuated’ is the only commercially available vaccination strategy to control the disease, field and vaccine strains of different serotypes could co-circulate. Additionally, in AHS-endemic countries, more than one serotype is often circulating at the same time. Therefore, a strategy to rapidly determine the virus serotype in an AHS-positive sample is strongly recommended in both epidemiological situations. The main objective of this study is to describe the development and validation of three triplex real-time RT-PCR (rRT-PCR) methods for rapid AHSV serotype detection. Samples from recent AHS outbreaks in Kenia (2015–2017), Thailand (2020), and Nigeria (2023), and from the AHS outbreak in Spain (1987–1990), were included in the study for the validation of these methods.

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Section: Discussionmentioning

confidence: 99%