Addressing K/L-edge overlap in elemental analysis from micro-X-ray fluorescence: bioimaging of tungsten and zinc in bone tissue using synchrotron radiation and laser ablation inductively coupled plasma mass spectrometry

VanderSchee, Cassidy R.; Kuter, David J.; Chou, Hsiang; Jackson, Brian P.; Mann, Koren K.; Bohle, D. Scott

doi:10.1007/s00216-019-02244-9

Cited by 12 publications

(3 citation statements)

References 27 publications

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“…Cytotoxicity Analysis. First, we analyzed the impact of CC, CC-Aln and CC-Trm NPs on cellular mitochondrial activity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in two cell cultures, EA.hy926 vascular endothelial cells and HepG2 hepatic cells, at three different times (24,48, and 72 h) and five different NP concentrations (results included in the Supporting Information). Next, we studied whether the observed low cytotoxicity was due to apoptosis or necrosis with the Annexin V Apoptosis Detection Kit APC (Ebioscience #88-8007-72).…”

Section: Discussionmentioning

confidence: 99%

“…To gain a deeper understanding of fundamental aspects of NP targeted imaging, we completed our study with synchrotron X-ray fluorescence (SXRF) analysis of arterial tissue. This technique provided elemental information on the targeted plaques and NP location in the aortic tissue, 24 revealing different behaviors in the temporal accumulation of NPs functionalized with different ligands. Our study provides insights into the field of targeted imaging of atherosclerosis and demonstrates the potential of advanced MRI contrast agents based on Gd-doped amorphous CC NPs for use as a safe and effective tool in comparative studies of targeted imaging.…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Study of Ultrasmall Calcium Carbonate Nanoparticles for Targeting and Imaging Atherosclerotic Plaque

et al. 2023

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Atherosclerosis is a complex disease that can lead to life-threatening events, such as myocardial infarction and ischemic stroke. Despite the severity of this disease, diagnosing plaque vulnerability remains challenging due to the lack of effective diagnostic tools. Conventional diagnostic protocols lack specificity and fail to predict the type of atherosclerotic lesion and the risk of plaque rupture. To address this issue, technologies are emerging, such as noninvasive medical imaging of atherosclerotic plaque with customized nanotechnological solutions. Modulating the biological interactions and contrast of nanoparticles in various imaging techniques, including magnetic resonance imaging, is possible through the careful design of their physicochemical properties. However, few examples of comparative studies between nanoparticles targeting different hallmarks of atherosclerosis exist to provide information about the plaque development stage. Our work demonstrates that Gd (III)-doped amorphous calcium carbonate nanoparticles are an effective tool for these comparative studies due to their high magnetic resonance contrast and physicochemical properties. In an animal model of atherosclerosis, we compare the imaging performance of three types of nanoparticles: bare amorphous calcium carbonate and those functionalized with the ligands alendronate (for microcalcification targeting) and trimannose (for inflammation targeting). Our study provides useful insights into ligand-mediated targeted imaging of atherosclerosis through a combination of in vivo imaging, ex vivo tissue analysis, and in vitro targeting experiments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Ultrasmall Calcium Carbonate Nanoparticles for Targeting and Imaging Atherosclerotic Plaque

et al. 2023

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“…However, for heavier elements, SR-mXRF may be less sensitive and accurate than LA-ICP-MS, especially when the incident X-ray beam cannot reach the K-edge of the desired element (e.g. tungsten and cadmium), requiring the use of the L-or M-edge that yield fewer fluorescent X-rays and may overlap with fluorescent X-rays from the K-edge of lighter elements (VanderSchee et al, 2020). Additionally, despite SR-mXRF being a powerful tool for answering questions of elemental localization and speciation, it is generally considered qualitative or semi-quantitative.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of quantitative synchrotron radiation micro-X-ray fluorescence in rice grain

Limmer¹,

Webb²,

Seyfferth³

2023

J Synchrotron Radiat

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Concentrations of nutrients and contaminants in rice grain affect human health, specifically through the localization and chemical form of elements. Methods to spatially quantify the concentration and speciation of elements are needed to protect human health and characterize elemental homeostasis in plants. Here, an evaluation was carried out using quantitative synchrotron radiation microprobe X-ray fluorescence (SR-µXRF) imaging by comparing average rice grain concentrations of As, Cu, K, Mn, P, S and Zn measured with rice grain concentrations from acid digestion and ICP-MS analysis for 50 grain samples. Better agreement was found between the two methods for high-Z elements. Regression fits between the two methods allowed quantitative concentration maps of the measured elements. These maps revealed that most elements were concentrated in the bran, although S and Zn permeated into the endosperm. Arsenic was highest in the ovular vascular trace (OVT), with concentrations approaching 100 mg kg−1 in the OVT of a grain from a rice plant grown in As-contaminated soil. Quantitative SR-µXRF is a useful approach for comparison across multiple studies but requires careful consideration of sample preparation and beamline characteristics.

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Distribution and accumulation of mercury in pot-grown African rice cultivars (Oryza glaberrima Steud. and Oryza sativa L.) determined via LA-ICP-MS

Punshon

Jackson

Donohue

et al. 2022

Environ Geochem Health

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Addressing K/L-edge overlap in elemental analysis from micro-X-ray fluorescence: bioimaging of tungsten and zinc in bone tissue using synchrotron radiation and laser ablation inductively coupled plasma mass spectrometry

Cited by 12 publications

References 27 publications

A Comparative Study of Ultrasmall Calcium Carbonate Nanoparticles for Targeting and Imaging Atherosclerotic Plaque

A Comparative Study of Ultrasmall Calcium Carbonate Nanoparticles for Targeting and Imaging Atherosclerotic Plaque

Evaluation of quantitative synchrotron radiation micro-X-ray fluorescence in rice grain

Distribution and accumulation of mercury in pot-grown African rice cultivars (Oryza glaberrima Steud. and Oryza sativa L.) determined via LA-ICP-MS

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