The anaerobic digestion process is an interesting method for the treatment of biochemical matter. Anaerobic inhibitors, such as high volatile fatty acids (VFAs) and sulphur may reduce the metabolic activity, and consequently cause digester failure. Nonetheless, metallic additives may help to reduce the influence of inhibition and improve the stability of the anaerobic process for improved methane and biogas productions. Therefore, this study investigated the effect of five (5) metallic additives (nanoparticles); iron (Fe-NPs), nickel (Ni-NPs), copper (Cu-NPs), zinc (Zn-NPs) and magnetite (Fe3O4-NPs) on the performance of the anaerobic digestion process. The experimental work was executed using 1 L digesters, with a working volume of 0.8 L and a headspace 0.2 L. The application of metallic additives in anaerobic digestion revealed higher biogas production and greater pollutant removals. The Fe3O4-NPs digester generated the highest biogas production of 260 ml/g VSfed and also showed higher removals for chemical oxygen demand, total suspended solids, total organic carbon, and ammonia-nitrogen. In terms of kinetics study, the Fe3O4-NPs digester revealed the shortest lag phase (1.9 days), highest coefficient of determination (0.95) and greatest hydrolysis constant rate (0.310/day). In conclusion, the metallic additives were successfully applied in anaerobic digestion for improving metabolic activity.