Additive methane-mitigating effect between linseed oil and nitrate fed to cattle1

Guyader, Jessie; Eugène, Maguy; Meunier, Bruno; Doreau, M.; Morgavi, Diego P.; Silberberg, Mathieu; Rochette, Yvanne; Gérard, Claude; Loncke, Christelle; Martin, Cécile

doi:10.2527/jas.2014-8196

Cited by 102 publications

(134 citation statements)

References 36 publications

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“…For each feed and refusals sample, one aliquot was used to determine DM content (103°C for 24 h) and the other aliquot was stored at 4°C (hay and concentrates) or −20°C (maize silage) until further analyses. Chemical composition analyses (ash, N, NDF, ADF, starch, GE, ether extract and fatty acid) were carried out on fresh (hay, concentrates) or freeze-dried (maize silage) samples after grinding (1 mm) (InVivo Labs, Chierry, France) and as previously described (Guyader et al, 2015). Juice from fresh maize silage was obtained by maceration to analyse pH, ammonia (N-NH 3 ; Kjeldahl method 2001.11;AOAC, 2005), acetic and lactic acid (gas chromatography with a flame ionization detector) concentrations (InVivo Labs).…”

Section: Measurements and Analysesmentioning

confidence: 99%

“…Daily enteric CH 4 emission of each animal was continuously measured for 2 consecutive days using open circuit respiration chambers (one animal per chamber) after 5 and 16 weeks of distribution of dietary treatments (Guyader et al, 2015). During measurement periods, chamber rear doors were opened in the morning for milking and to remove faeces and urine, and in the afternoon for milking.…”

Section: Measurements and Analysesmentioning

confidence: 99%

“…Total tract apparent digestibility and N balance were determined from total and separate collection of faeces and urine for 5 days during week 17 (Guyader et al, 2015). At the end of week 16, cows were moved from CH 4 chambers to individual digestibility crates to give animals a 3-day adaptation period to new housing conditions before the first collection.…”

Section: Measurements and Analysesmentioning

confidence: 99%

“…One study reported a lower DMI (−7%) by lactating cows fed a grass silage-based diet supplemented with linseed (3% added fat, Martin et al, 2011). The only study that simultaneously used linseed plus nitrate (4% added fat plus 2.3% nitrate) on cows did not result in intake changes, but the cows were non-lactating and restricted-fed (Guyader et al, 2015). We hypothesise that LIN + NIT fed together ad libitum may have an inhibitory effect on voluntary intake linked to a tendency for lower ADF digestibility.…”

mentioning

confidence: 92%

“…In a short-term experiment on non-lactating cows, linseed oil (4% of dry matter; DM) plus nitrate (2.25% of DM) reduced methanogenesis by 32% without affecting apparent diet digestibility (Guyader et al, 2015). Compared to linseed oil and nitrate fed individually, the effect of this combination on CH 4 production was additive because these two strategies share different modes of action in the rumen: polyunsaturated lipids from linseed are thought to act as inhibitors of H 2 producers such as protozoa, whereas nitrate is thought to act as a H 2 sink, competing with methanogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Long-term effect of linseed plus nitrate fed to dairy cows on enteric methane emission and nitrate and nitrite residuals in milk

Guyader

Doreau

Morgavi

et al. 2016

Animal

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A previous study showed the additive methane (CH 4 )-mitigating effect of nitrate and linseed fed to non-lactating cows. Before practical application, the use of this new strategy in dairy cows requires further investigation in terms of persistency of methanogenesis reduction and absence of residuals in milk products. The objective of this experiment was to study the long-term effect of linseed plus nitrate on enteric CH 4 emission and performance in dairy cows. We also assessed the effect of this feeding strategy on the presence of nitrate residuals in milk products, total tract digestibility, nitrogen (N) balance and rumen fermentation. A total of 16 lactating Holstein cows were allocated to two groups in a randomised design conducted in parallel for 17 weeks. Diets were on a dry matter (DM) basis: (1) control (54% maize silage, 6% hay and 40% concentrate; CON) or (2) control plus 3.5% added fat from linseed and 1.8% nitrate (LIN + NIT). Diets were equivalent in terms of CP (16%), starch (28%) and NDF (33%), and were offered twice daily. Cows were fed ad libitum, except during weeks 5, 16 and 17 in which feed was restricted to 95% of dry matter intake (DMI) to ensure complete consumption of meals during measurement periods. Milk production and DMI were measured weekly. Nitrate and nitrite concentrations in milk and milk products were determined monthly. Daily CH 4 emission was quantified in open circuit respiration chambers (weeks 5 and 16). Total tract apparent digestibility, N balance and rumen fermentation parameters were determined in week 17. Daily DMI tended to be lower with LIN + NIT from week 4 to 16 (−5.1 kg/day on average). The LIN + NIT diet decreased milk production during 6 non-consecutive weeks (−2.5 kg/day on average). Nitrate or nitrite residuals were not detected in milk and associated products. The LIN + NIT diet reduced CH 4 emission to a similar extent at the beginning and end of the trial (−47%, g/day; −30%, g/kg DMI; −33%, g/kg fat-and protein-corrected milk, on average). Diets did not affect N efficiency and nutrients digestibility. In the rumen, LIN + NIT did not affect protozoa number but reduced total volatile fatty acid (−12%) and propionate (−31%) concentrations. We concluded that linseed plus nitrate may have a long-term CH 4 -mitigating effect in dairy cows and that consuming milk products from cows fed nitrate may be safe in terms of nitrate and nitrite residuals. Further work is required to optimise the doses of linseed plus nitrate to avoid reduced cows performance.

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Section: Measurements and Analysesmentioning

confidence: 99%

Section: Measurements and Analysesmentioning

confidence: 99%

Section: Measurements and Analysesmentioning

confidence: 99%

mentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Long-term effect of linseed plus nitrate fed to dairy cows on enteric methane emission and nitrate and nitrite residuals in milk

Guyader

Doreau

Morgavi

et al. 2016

Animal

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Defatted silkworm pupae meal as an alternative protein source for cattle

Rashmi

Soren

Prasad

et al. 2022

Trop Anim Health Prod

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Review of current in vivo measurement techniques for quantifying enteric methane emission from ruminants

Hammond

Crompton

Bannink

et al. 2016

Animal Feed Science and Technology

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Review of current in vivo measurement techniques for quantifying enteric methane emission from ruminants.Animal Feed Science and Technology http://dx.doi.org/10. 1016/j.anifeedsci.2016.05.018 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. techniques based on short-term measurements of gas concentrations in samples of exhaled air. This includes automated head chambers (e.g. the GreenFeed system), the use of carbon dioxide (CO 2 ) as a marker, and (handheld) laser CH 4 detection. Each of the techniques are compared and assessed on their capability and limitations, followed by methodology recommendations. It is concluded that there is no "one size fits all" method for measuring CH 4 emission by individual animals. Ultimately, the decision as to which method to use should be based on the experimental objectives and resources available. However, the need for high throughput methodology e.g. for screening large numbers of animals for genomic studies, does not justify the use of methods that are inaccurate. All CH 4 measurement techniques are subject to experimental variation and random errors. Many sources of variation must be considered when measuring CH 4 concentration in exhaled air samples without a quantitative or at least regular collection rate, or use of a marker to indicate (or adjust) for the proportion of exhaled CH 4 sampled. Consideration of the number and timing of measurements relative to diurnal patterns of CH 4 emission and respiratory exchange are important, as well as consideration of feeding patterns and associated patterns of rumen 4 fermentation rate and other aspects of animal behaviour. Regardless of the method chosen, appropriate calibrations and recovery tests are required for both method establishment and routine operation. Successful and correct use of methods requires careful attention to detail, rigour, and routine self-assessment of the quality of the data they provide.

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Additive methane-mitigating effect between linseed oil and nitrate fed to cattle1

Cited by 102 publications

References 36 publications

Long-term effect of linseed plus nitrate fed to dairy cows on enteric methane emission and nitrate and nitrite residuals in milk

Long-term effect of linseed plus nitrate fed to dairy cows on enteric methane emission and nitrate and nitrite residuals in milk

Defatted silkworm pupae meal as an alternative protein source for cattle

Review of current in vivo measurement techniques for quantifying enteric methane emission from ruminants

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