Additive manufacturing of barium-doped calcium silicate/poly-ε-caprolactone scaffolds to activate CaSR and AKT signalling and osteogenic differentiation of mesenchymal stem cells

Chiu, Yung-Cheng; Lin, Cheng-Yao; Chen, Yiwen; Kuo, Ting-You; Shie, Ming‐You

doi:10.1039/d3tb00208j

Cited by 6 publications

(3 citation statements)

References 56 publications

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“…Moreover, the Ca 2+ concentration played a critical role in this process. This factor can regulate osteoblast differentiation by activating calcium-sensing receptors and increasing Ca 2+ influx into osteoblasts (Chiu et al, 2023). In addition to these potential reasons, a recent study reported that Cu 2+ could promote higher expression of osteogenesis-related genes, and mineralization of the cells (Burghardt et al, 2015).…”

Section: Scaffolds Promote Osteogenic Differentiation Of Bmscs In Vitromentioning

confidence: 99%

Calcium sulfate-Cu2+ delivery system improves 3D-Printed calcium silicate artificial bone to repair large bone defects

Gao,

Li,

Lei

et al. 2023

Front. Bioeng. Biotechnol.

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There are still limitations in artificial bone materials used in clinical practice, such as difficulty in repairing large bone defects, the mismatch between the degradation rate and tissue growth, difficulty in vascularization, an inability to address bone defects of various shapes, and risk of infection. To solve these problems, our group designed stereolithography (SLA) 3D-printed calcium silicate artificial bone improved by a calcium sulfate-Cu2+ delivery system. SLA technology endows the scaffold with a three-dimensional tunnel structure to induce cell migration to the center of the bone defect. The calcium sulfate-Cu2+ delivery system was introduced to enhance the osteogenic activity of calcium silicate. Rapid degradation of calcium sulfate (CS) induces early osteogenesis in the three-dimensional tunnel structure. Calcium silicate (CSi) which degrades slowly provides mechanical support and promotes bone formation in bone defect sites for a long time. The gradient degradation of these two components is perfectly matched to the rate of repair in large bone defects. On the other hand, the calcium sulfate delivery system can regularly release Cu2+ in the temporal and spatial dimensions, exerting a long-lasting antimicrobial effect and promoting vascular growth. This powerful 3D-printed calcium silicate artificial bone which has rich osteogenic activity is a promising material for treating large bone defects and has excellent potential for clinical application.

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Section: Scaffolds Promote Osteogenic Differentiation Of Bmscs In Vitromentioning

confidence: 99%

Calcium sulfate-Cu2+ delivery system improves 3D-Printed calcium silicate artificial bone to repair large bone defects

Gao,

Li,

Lei

et al. 2023

Front. Bioeng. Biotechnol.

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“…Recently, significant attention has been directed toward the role of barium in bone repair and regeneration, particularly in the context of osteogenesis [24][25][26]. It has been demonstrated that the incorporation of Ba 2+ into bioceramic compositions enhances the mineralization of their surfaces under in vitro conditions, as observed during immersion in simulated body fluid (SBF) [26][27][28][29][30]. One such oxide ceramic mineral phase, which includes silicon, calcium, and barium, is known as walstromite.…”

Section: Introductionmentioning

confidence: 99%

New 3D Printed Scaffolds Based on Walstromite Synthesized by Sol–Gel Method

Chiriac,

Popescu,

Pele

et al. 2024

JFB

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This study explores the potential utilization of walstromite (BaCa2Si3O9) as a foundational material for creating new bioceramics in the form of scaffolds through 3D printing technology. To achieve this objective, this study investigates the chemical–mineralogical, morphological, and structural characteristics, as well as the biological properties, of walstromite-based bioceramics. The precursor mixture for walstromite synthesis is prepared through the sol–gel method, utilizing pure reagents. The resulting dried gelatinous precipitate is analyzed through complex thermal analysis, leading to the determination of the optimal calcination temperature. Subsequently, the calcined powder is characterized via X-ray diffraction and scanning electron microscopy, indicating the presence of calcium and barium silicates, as well as monocalcium silicate. This powder is then employed in additive 3D printing, resulting in ceramic scaffolds. The specific ceramic properties of the scaffold, such as apparent density, absorption, open porosity, and compressive strength, are assessed and fall within practical use limits. X-ray diffraction analysis confirms the formation of walstromite as a single phase in the ceramic scaffold. In vitro studies involving immersion in simulated body fluid (SBF) for 7 and 14 days, as well as contact with osteoblast-like cells, reveal the scaffold’s ability to form a phosphate layer on its surface and its biocompatibility. This study concludes that the walstromite-based ceramic scaffold exhibits promising characteristics for potential applications in bone regeneration and tissue engineering.

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“…Three-dimensional (3D) printing is often used for industrial applications due to its flexibility and capability to provide ample mechanical strength and support after fabrication [ 1 ]. Furthermore, 3D printing is cost- and time-efficient and can provide design versatility [ 2 , 3 , 4 ]. It can fabricate scaffolds with lattice-like structures, providing weight reduction and ample mechanical strength and stiffness for numerous engineering applications such as automobile, aerospace, and biomedical engineering [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancing the Mechanical Properties and Aging Resistance of 3D-Printed Polyurethane through Polydopamine and Graphene Coating

Tung,

Lin,

Chen

et al. 2023

Polymers

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Three-dimensional (3D) printing is a versatile manufacturing method widely used in various industries due to its design flexibility, rapid production, and mechanical strength. Polyurethane (PU) is a biopolymer frequently employed in 3D printing applications, but its susceptibility to UV degradation limits its durability. To address this issue, various additives, including graphene, have been explored to enhance PU properties. Graphene, a two-dimensional carbon material, possesses remarkable mechanical and electrical properties, but challenges arise in its dispersion within the polymer matrix. Surface modification techniques, like polydopamine (PDA) coating, have been introduced to improve graphene’s compatibility with polymers. This study presents a method of 3D printing PU scaffolds coated with PDA and graphene for enhanced UV stability. The scaffolds were characterized through X-ray diffraction, Fourier-transform infrared spectroscopy, mechanical testing, scanning electron microscopy, and UV durability tests. Results showed successful PDA coating, graphene deposition, and improved mechanical properties. The PDA–graphene-modified scaffolds exhibited greater UV resistance over time, attributed to synergistic effects between PDA and graphene. These findings highlight the potential of combining PDA and graphene to enhance the stability and mechanical performance of 3D-printed PU scaffolds.

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Additive manufacturing of barium-doped calcium silicate/poly-ε-caprolactone scaffolds to activate CaSR and AKT signalling and osteogenic differentiation of mesenchymal stem cells

Abstract: The 3D-printed scaffold is suitable for patient-specific implant preparation for bone regeneration in large-scale critical bone defects. In addition, these scaffolds should have mechanical and biological properties similar to those...

Cited by 6 publications

References 56 publications

Calcium sulfate-Cu2+ delivery system improves 3D-Printed calcium silicate artificial bone to repair large bone defects

Calcium sulfate-Cu2+ delivery system improves 3D-Printed calcium silicate artificial bone to repair large bone defects

New 3D Printed Scaffolds Based on Walstromite Synthesized by Sol–Gel Method

Enhancing the Mechanical Properties and Aging Resistance of 3D-Printed Polyurethane through Polydopamine and Graphene Coating

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