Additive inhibition of lordosis by simultaneous treatments with GABAA and GABAB receptor agonists, muscimol and baclofen, in female rats

Wada, Shizuko; Yamada, Shunji; Yamanouchi, Korehito

doi:10.1016/j.pbb.2008.04.020

Cited by 7 publications

(3 citation statements)

References 39 publications

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“…In addition, half of mating-induced cFos-expressing neurons were not glutamatergic in the lateral PAG. GABA- or neurotensin-expressing neural cell bodies are present in the PAG (Paxinos, 2004 ) and GABA is involved in lordosis behavior (Wada et al, 2008 ). This indicates that GABA neurons are a candidate for the neurons activated by mating stimulus in the lateral PAG.…”

Section: Discussionmentioning

confidence: 99%

Identification of neural cells activated by mating stimulus in the periaqueductal gray in female rats

Yamada

Kawata

2014

Front. Neurosci.

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Induction of lordosis as typical female sexual behavior in rodents is dependent on a mount stimulus from males and blood levels of estrogen. Periaqueductal gray (PAG) efferent neurons have been suggested to be important for lordosis behavior; however, the neurochemical basis remains to be understood. In this study, we neuroanatomically examined (1) whether PAG neurons activated by mating stimulus project to the medullary reticular formation (MRF), which is also a required area for lordosis; and (2) whether these neurons are glutamatergic. Mating stimulus significantly increased the number of cFos-immunoreactive (ir) neurons in the PAG, particularly in its lateral region. Half of cFos-ir neurons in the lateral PAG were positive for a retrograde tracer (FluoroGold; FG) injected into the MRF. cFos-ir neurons also colocalized with mRNA of vesicular glutamate transporter 2 (vGLUT2), a molecular marker for glutamatergic neurons. Using retrograde tracing and in situ hybridization in conjunction with fluorescent microscopy, we also found FG and vGLUT2 mRNA double-positive neurons in the lateral PAG. These results suggest that glutamatergic neurons in the lateral PAG project to the MRF and are involved in lordosis behavior in female rats.

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Section: Discussionmentioning

confidence: 99%

Identification of neural cells activated by mating stimulus in the periaqueductal gray in female rats

Yamada

Kawata

2014

Front. Neurosci.

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“…The fact that both GABA A and GABA B antagonists have similar effects, and that both GABA A and GABA B agonists have the opposite effect, is not particularly surprising. It has repeatedly been shown, for example, that GABA A and GABA B agonists inhibit the display of lordosis in female rats independently of their actions on motor functions . In addition, administration of muscimol into the ventrolateral periaquductal gray inhibits kyphosis (i.e.…”

Section: Discussionmentioning

confidence: 99%

GABA Receptors in the Medial Preoptic Area Modulate the Onset of Oestradiol‐Induced Maternal Behaviour in Hysterectomised‐Ovariectomised, Pregnant Rats

Gómora‐Arrati

Domínguez

Ågmo

2016

J Neuroendocrinology

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We studied the participation of GABA neurotransmission in the medial preoptic area (mPOA) with respect to the onset of the pup retrieval response and nest building. Pregnant female rats were implanted with bilateral cannulae in the mPOA on day 12 of pregnancy and, on day 16, the females were hysterectomised and ovariectomised and given 200 μg/kg of oestradiol benzoate. Two days later, the females received one of the following intracerebral drug treatments: GABA agonist baclofen (200 ng); GABA antagonist phaclofen (1 μg); GABA antagonist bicuculline (60 ng); or physiological saline. Five minutes after intracerebral infusion, three foster pups were introduced into the females' home cage. The subjects were observed for pup grouping (retrieval) during 15 min, after which the pups were left with the female. During the next 12 h, an observation was made every 1 h to determine whether the pups had been grouped (retrieved) or not. The GABA agonist baclofen reduced the proportion of females retrieving pups from 4 to 8 h following pup introduction. By contrast, both the GABA antagonist bicuculline and the GABA antagonist phaclofen enhanced the proportion of females retrieving pups during the first 3 h of observation. The latency to pup retrieval in subjects treated with the GABA agonist baclofen was significantly longer than that in subjects given any of the antagonists. All females built a nest but baclofen reduced nest quality. These data show that activation of GABA receptors in the mPOA has an inhibitory effect on basic maternal behaviours, whereas blockade of either the GABA or GABA receptor facilitates pup retrieval. It is possible that reduced GABAergic tone in the mPOA is a key element in the initiation of maternal behaviours in postparturient rats.

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“…Several lines of research demonstrate that GABA levels are associated with sexual function. Animal studies show that GABA(A) and GABA(B) receptors are involved in the inhibition of lordosis (a response shown by female animals indicating sexual receptivity) as well as mediating the effects of sex steroids such as estrogen in appetitive sexual behavior (e.g., sexual exploration) [37] , [38] . Elevated levels of stress have also been shown to dampen sexual response in animal models as well as being a significant psychological correlate of FSD in women [39] , [40] .…”

Section: Discussionmentioning

confidence: 99%

A Genome-Wide Association Study of Female Sexual Dysfunction

Burri

Hysi²,

Clop³

et al. 2012

PLoS ONE

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BackgroundFemale sexual dysfunction (FSD) is an important but controversial problem with serious negative impact on women’s quality of life. Data from twin studies have shown a genetic contribution to the development and maintenance of FSD.Methodology/Principal FindingsWe performed a genome-wide association study (GWAS) on 2.5 million single-nucleotide polymorphisms (SNPs) in 1,104 female twins (25–81 years of age) in a population-based register and phenotypic data on lifelong sexual functioning. Although none reached conventional genome-wide level of significance (10×-8), we found strongly suggestive associations with the phenotypic dimension of arousal (rs13202860, P = 1.2×10−7; rs1876525, P = 1.2×10−7; and rs13209281 P = 8.3×10−7) on chromosome 6, around 500kb upstream of the locus HTR1E (5-hydroxytryptamine receptor 1E) locus, related to the serotonin brain pathways. We could not replicate previously reported candidate SNPs associated with FSD in the DRD4, 5HT2A and IL-1B loci.Conclusions/SignificanceWe report the first GWAS of FSD symptoms in humans. This has pointed to several “risk alleles” and the implication of the serotonin and GABA pathways. Ultimately, understanding key mechanisms via this research may lead to new FSD treatments and inform clinical practice and developments in psychiatric nosology.

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Additive inhibition of lordosis by simultaneous treatments with GABAA and GABAB receptor agonists, muscimol and baclofen, in female rats

Cited by 7 publications

References 39 publications

Identification of neural cells activated by mating stimulus in the periaqueductal gray in female rats

Identification of neural cells activated by mating stimulus in the periaqueductal gray in female rats

GABA Receptors in the Medial Preoptic Area Modulate the Onset of Oestradiol‐Induced Maternal Behaviour in Hysterectomised‐Ovariectomised, Pregnant Rats

A Genome-Wide Association Study of Female Sexual Dysfunction

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