Additive effects of Capsaicin Oleoresin, Irbesartan and Amlodipine Besylate on the blood pressure of spontaneously hypertensive rats

Abstract

The effects of oral intake of capsaicin oleoresin (CO) on arterial blood pressure was examined in male spontaneously hypertensive (SHR) rats. Animals were randomized into control (C), capsaicin oleoresin (CO), capsaicin oleoresin-irbesartan (COI) and capsaicin oleoresin-amlodipine besylate (COAB) fed groups. The experimental groups received 50 mg of Capsaicin Oleoresin only, CO with 25 mg of Irbesartan, and CO with 2.5 mg of Amlodipine per kilogram of body weight every other day for 4 weeks. Blood pressure was measured weekly by tail-cuff for 4 weeks. Biological samples were obtained at baseline, day 18, and day 34 (terminal) for aldosterone, arginine vasopressin (AVP), renin, 6-Keto-PGF-1 α , Prostaglandin E 2 , catecholamines, adrenocorticotropic hormone (ACTH) levels, and serum minerals. Statistical significance was set at p≤0.05. The results trended towards decreasing (p=0.190) systolic blood pressure (SBP) in the COAB and CO compared to the COI and control groups. However, significance among group differences were observed for urine aldosterone and AVP, and serum sodium, calcium, magnesium, potassium, and catecholamine (p≤0.05), but not for ACTH, renin, and 6-Keto-PGF-1 α levels. Capsaicin treatment was non-significantly associated with decreased SBP in SHR. These findings suggested that the effects of Capsaicin on SPB may involve aldosterone and arteriolar vascular tone.