Additions to the technique of epi-illumination light microscopy for the study of floral and vegetative apices

Charlton, W. A.; MacDonald, A. D.; Posluszny, Usher; Wilkins, C. P.

doi:10.1139/b89-220

Cited by 52 publications

(26 citation statements)

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“…The material was immediately fixed in FAA (5 parts formalin, 5 parts glacial acetic acid, 90 parts 50% ethanol). After a fixation period of 24 h, the samples were rinsed, dehydrated in 70% ethanol and further dehydrated to 95% ethanol prior to being stained with alcohol soluble nigrosin (0.4% nigrosin in 95% ethanol) for at least 2 days according to the method reported by Charlton et al (1989). The specimens were washed in 95% ethanol before microscopic evaluations.…”

Section: Methodsmentioning

confidence: 99%

Floral ontogeny in Astragalus compactus (Leguminosae: Papilionoideae: Galegeae): variable occurrence of bracteoles and variable patterns of sepal initiation

Naghiloo

Dadpour

Movafeghi

2011

Planta

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Comparative studies of floral ontogeny represent a growing field that promise to provide new insights on floral evolution. Floral ontogenetic information has been used successfully in Leguminosae for re-examining phylogenetic relationships at different levels. Using epi-illumination light microscopy, we present original ontogenetic data in Astragalus compactus, which was chosen because of its unusual arrangement of inflorescence and variable occurrence of bracteoles on flowers. Based on our results, uncommon ontogeny of the inflorescence led to the arrangement of flowers in four different positions. Variation was observed not only in the presence of bracteoles, but also in the order of sepal initiation in flowers of the same inflorescence. Surprisingly, besides the widely stated unidirectional pattern, bidirectional, sequential and an atypical unreported order were observed. High degree of overlapping between whorls and formation of two types of common primordia also were found. The variable occurrence of bracteoles suggests that the species is in an intermediate state towards fully lacking of bracteoles. We propose that the variability of the sequence of sepal initiation is possibly a consequence of the function of mechanical forces generated by surrounding leaves. Relationships between mechanical force and auxin signalling are discussed.

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Section: Methodsmentioning

confidence: 99%

Floral ontogeny in Astragalus compactus (Leguminosae: Papilionoideae: Galegeae): variable occurrence of bracteoles and variable patterns of sepal initiation

Naghiloo

Dadpour

Movafeghi

2011

Planta

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“…The prepared samples were dissected under a SZX 9 stereomicroscope (Olympus, Tokyo, Japan) and stained with alcohol soluble nigrosin (Merck, Darmstadt, Germany) according to a previous report (Charlton et al 1989). Further washing of the samples was carried out in 95 % ethanol for 2 d. Nigrosin stained apices were examined using an Olympus BX60 microscope equipped with U-URBL illuminator and U-RLBC mirror cassette.…”

Section: Methodsmentioning

confidence: 99%

Determination of floral initiation in Malus domestica: a novel morphogenetic approach

Dadpour¹,

Movafeghi²,

Grigorian³

et al. 2011

Biologia plant.

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Floral initiation in apple (Malus domestica Borkh) was studied by a novel morphogenetic approach. Developmental stages of apices were evaluated based on the morphology of shoot apical meristem (SAM) from various collection dates. Besides, the frequency of each stage was calculated within apices populations after full blooming (DAFB). Prior to doming of apex, three marked phases were found based on SAM morphology: 1) narrow appearance (vegetative phase), 2) broadened form (transition phase), and 3) prominent shape (commitment phase). A furrow region was formed at the base of leaf primordium during the bract initiation, while significant broadening of SAM was observed. Cell division patterns manifested in modification of anisotropic clusters from isotropic cellular packets, as a result of which profound morphological changes of apices occurred. Based on these findings, we propose that the structural alterations prior to doming may be taken into account for determination of the initial development and reproduction signs in apple trees.Additional key words: apple, cell division pattern, epi-illumination light microscopy, fluorescence microscopy, shoot apical meristem.

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“…The material was fixed in FAA (5 parts formalin, 5 parts glacial acetic acid, 90 parts 50% ethanol) for at least 24 h. The specimens were then rinsed, dehydrated in 70% ethanol and further dehydrated in 95% ethanol. Afterwards, they were stained with alcohol soluble nigrosin (0.4% nigrosin in 95% ethanol) for at least two days according to the method reported by Charlton et al (1989). The samples were washed in 95% ethanol before microscopic examinations.…”

Section: Source Of Materialsmentioning

confidence: 99%

Inflorescence and floral ontogeny in Crocus sativus L. (Iridaceae)

Dadpour

Naghiloo

Gohari

et al. 2012

Flora - Morphology, Distribution, Functional Ecology of Plants

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Additions to the technique of epi-illumination light microscopy for the study of floral and vegetative apices

Cited by 52 publications

References 0 publications

Floral ontogeny in Astragalus compactus (Leguminosae: Papilionoideae: Galegeae): variable occurrence of bracteoles and variable patterns of sepal initiation

Floral ontogeny in Astragalus compactus (Leguminosae: Papilionoideae: Galegeae): variable occurrence of bracteoles and variable patterns of sepal initiation

Determination of floral initiation in Malus domestica: a novel morphogenetic approach

Inflorescence and floral ontogeny in Crocus sativus L. (Iridaceae)

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