Additional species of Aspergillus causing bole rot disease in Agave sisalana

Santos, Patrícia; Silva, Augusto César Moura da; Corrêa, Élida Barbosa; Magalhães, Valter Cruz; Souza, Jorge Teodoro de

doi:10.1590/s1982-56762014000400008

Cited by 10 publications

(9 citation statements)

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“…These symptoms were described as yellowish leaves (external symptoms) and brown internal tissue surrounded by reddish tissue inside sisal aerial stem (internal symptoms) (Coutinho et al, 2006 ). Santos et al ( 2014 ) reported that A. niger, Aspergillus brasiliensis , and Aspergillus tubingensis , isolated from the soils of sisal-producing areas, were able to cause this disease in sisal plants inoculated under greenhouse conditions. Nevertheless, these authors pointed out that only A. niger was isolated from field symptomatic plants, and the epidemiological significance of the other two Aspergillus species remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil

et al. 2018

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Approximately 75% of the worldwide production of hard natural fibers originates from sisal, an industrial crop from arid and semiarid tropical regions. Brazil is the world's largest producer of sisal fiber, accounting for more than 40% of the worldwide production, and sisal bole rot disease has been the main phytosanitary problem of this crop. All previous studies reporting Aspergillus niger as the causal agent of the disease were based on the morphological features of fungal isolates from infected plant tissues in pure cultures. Black aspergilli are one of the most complex and difficult groups to classify and identify. Therefore, we performed an integrative analysis of this disease based on the isolation of black aspergilli from the endospheres and soils in the root zones of symptomatic adult plants, in vivo pathogenicity tests, histopathology of symptomatic plants, and molecular phylogeny and worldwide genetic variability of the causal agent. All sisal isolates were pathogenic and unequivocally produced symptoms of bole rot disease in healthy plants. In all tree-based phylogenetic methods used, a monophyletic group formed by A. welwitschiae along with all sisal isolates was retrieved. Ten A. welwitschiae haplotypes have been identified in the world, and three occur in the largest sisal-producing area. Most of the isolates are from a unique haplotype, present in only the sisal-producing region. A. welwitschiae destroyed parenchymatic and vascular cylinder cells and induced the necrosis of internal stem tissues. Therefore, sisal bole disease is probably the consequence of a saprotrophic fungus that opportunistically invades sisal plants and behaves as a typical necrotrophic pathogen.

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Section: Introductionmentioning

confidence: 99%

Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil

et al. 2018

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“…Tabela 1 -Frequência de ocorrência de fungos associados à Bromeliaceae na Serra da Jibóia, Morro da Redenção e Orquilândia Tropical, estado da Bahia. As cinco espécies de fungos comuns aos locais estudados são frequentemente encontradas em outros hospedeiros, sendo amplamente registradas (Costa et al 2009;Reis et al 2009;Costa et al 2012;Mussi-Dias et al 2012;Santos et al 2014;Costa & Gusmão 2016;Candeias et al 2016).…”

Section: Resultsunclassified

Parâmetros ecológicos de fungos em Bromeliaceae em ecossistemas naturais e cultivadas na Bahia

Palha

Silva

et al. 2018

Rodriguésia

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Resumo Entre maio/2015 e fevereiro/2016 foi realizado um estudo objetivando estimar a riqueza, frequência e similaridade fúngica associada a Bromeliaceae de três áreas no estado da Bahia: Serra da Jibóia (município de Santa Terezinha), Morro da Redenção (município de Campo Formoso) e Orquilândia Tropical (município de Camaçari). Foram coletadas amostras de três espécies de Bromeliaceae por área: Alcantarea nahoumii, Vriesea bahiana, Aechmea sp. (Serra da Jibóia); Aechmea aquilega, Hohenbergia catingae, Tillandsia gardneri (Morro da Redenção) e Aechmea victoriana, A. naohoumii, Neoregelia compacta (Orquilândia Tropical). Foram selecionados dez indivíduos de cada espécie, destes, três folhas com sintomas ou sinais de fungos foram coletadas. Em laboratório efetuou-se o isolamento indireto dos fragmentos de folhas com desinfestação em álcool 70%, NaOCl 1% e lavagens em água destilada. Foram obtidos 19 gêneros distribuídos em 27 táxons. Na Orquilândia Tropical obteve-se a maior riqueza de espécies (17 táxons), as demais áreas apresentaram 14 táxons. Houve baixa similaridade fúngica nas plantas estudadas: 16,6% a 50% - Serra da Jibóia, 33,3% a 58,8% - Morro da Redenção e 23,3% a 58,3% - Orquilândia Tropical. Constatou-se diversidade fúngica para as espécies de bromélias tanto em ecossistemas naturais como em áreas cultivadas.

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“…Isolate AN18 of A. niger obtained by Santos et al (2014) from a diseased sisal plant was used in all experiments. The cultivation of A. niger was done in two culture media: 1) one minimal medium (MM) composed of 10 g sucrose, 2 g NaNO 3 , 1 g KH 2 PO 4 , 0.5 g MgSO 4 .7H 2 O, and 0.5 g KCl per litre plus 0.2 mL of a stock solution prepared with citric acid 5 g, ZnSO 4 .6H 2 O 5 g, Fe(NH 4 )2(SO 4 )2.6H 2 O 1 g, CuSO 4 .5H 2 O 250 mg, MnSO 4 50 mg, H 3 BO 3 (Boric Acid) 50 mg, Na 2 MoO 4 .2H 2 O 50 mg and distilled water 95 mL; and 2) the same medium amended with 1% of sisal extract (MS) before autoclaving at 120 ºC for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…The disease known as bole rot is one of the main factors leading to the sisal decadence. Although A. niger Thiegh, has been initially pointed out as the sole cause of the disease, other species in the section Nigri, such as A. brasiliensis and A. tubingensis are also pathogenic to sisal (Santos et al, 2014). This disease is considered to be the most important phytosanitary problem of sisal in Brazil by producers, extensionists and scientists due to its high destructive potential.…”

Section: Introductionmentioning

confidence: 99%

Putative pathogenicity genes of Aspergillus niger in sisal and their expression in vitro

Souza

Jesus

Santos

2017

Agraria

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Sisal (Agave sisalana Perrine ex Engelm) is cultivated in large areas of the Brazilian semi-arid Northeastern region. This crop allows thousands of people to obtain minimal wages. However, a disease known as bole rot, caused by Aspergillus species, is causing a decline in production. The objective of this study was to investigate the involvement of hydrolytic enzymes (cellulase, cutinase, protease and lipase) and ochratoxin A in an in vitro simulation of the interaction between A. niger Tiegh. and sisal to understand the mechanisms of fungal pathogenicity. Primers for cutinase lipase, protease, ochratoxin A, and elongation factor 1-α (EF), with this last one used as endogenous control were designed and optimized for real time quantitative PCR (qPCR) with the SYBR Green methodology. The genes potentially involved in the pathogenicity of A. niger showed higher expression in medium supplemented with sisal as compared to the minimal medium. The primers reported herein will allow detailed studies of the biological events leading to bole rot disease in sisal. These results represent the first data on genes putatively involved in the pathogenicity of A. niger to sisal.

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Additional species of Aspergillus causing bole rot disease in Agave sisalana

Cited by 10 publications

References 11 publications

Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil

Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil

Parâmetros ecológicos de fungos em Bromeliaceae em ecossistemas naturais e cultivadas na Bahia

Putative pathogenicity genes of Aspergillus niger in sisal and their expression in vitro

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