Additional glucoamylase genes increase ethanol productivity on rice and potato waste streams by a recombinant amylolytic yeast

Cripwell, Rosemary A.; My, Rebecca; Treu, Laura; Campanaro, Stefano; Favaro, Lorenzo; van Zyl, Willem H.; Viljoen-Bloom, Marinda

doi:10.1016/j.biortech.2023.129787

Cited by 3 publications

(1 citation statement)

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“…5 Previous research has shown that lipid-accumulating microorganisms are ideal for biodiesel production because they accumulate lipids in the form of triacylglycerol, which is transesterified into fatty acid methyl esters (FAME) to produce biodiesel. 6 Microorganisms use lignocellulosic waste to produce biofuel that is comparable to existing fuels and suitable for diesel engines. 7 Fatty acid ethyl esters (FAEE) were used to create recombinant E. coli-based microbial biodiesel in early 2006.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Production from Rice-Wash Water with Streptomyces fradiae Fermentation: Improved Free Fatty Acid Concentration

Kumar,

Anandan,

Kaliyaperumal

2024

J. Pure Appl. Microbiol.

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The lipid-producing gram-positive bacteria were isolated from soil and it was identified as Streptomyces fradiae JJ1 MK733985.1. Bacterial lipid synthesis was explored using rice washed water through the fermentation research. The exponential growth phase was reached on the fifth day of fermentation, according to the results. The ability of Streptomyces fradiae to produce microbial oil was compared to that of International Streptomyces Project-2 Medium (ISP2) medium. The results showed that RWW culture medium yielded 85.4% fatty acids and 60.3 g/L biomass, while ISP2 medium yielded 83.2% wt fatty acids and 59.5 g/L biomass. The microbial oil was transesterified into Fatty acid methyl esters (FAME), and its characteristics were studied using American Society for Testing Materials (ASTM) standards. The specific gravity was 0.864, the density was 0.863, the gross heating value was 42498 kj/kg, the cetane number was 37, and the acid value was 0.46. Streptomyces fradiae grew with an ISP2 density of 0.835, specific gravity of 0.845, gross heating value of 42326 kj/kg, and acid value of 0.43. According to the findings, Streptomyces fradiae isolated from soil can grow in RWW medium for the first time and provide the same yield as commercial media while being significantly less expensive.

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Section: Introductionmentioning

confidence: 99%