Additional evidence for recent divergence of Chinese Epimedium (Berberidaceae) derived from AFLP, chloroplast and nuclear data supplemented with characterisation of leaflet pubescence

Smet, Yannick De; Goetghebeur, Paul; Wanke, Stefan; Asselman, Pieter; Samain, Marie‐Stéphanie

doi:10.5091/plecevo.2012.646

Cited by 20 publications

(23 citation statements)

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“…In this study, the results showed that E. wushanense can be distinguished from its relative species (Figure 7). Phylogenetic analysis based on karyomorphology [31] and molecular markers [4,32] has been done to confirm the evolution of this genus. However, the further subdivision of the Chinese sect.…”

Section: Resultsmentioning

confidence: 99%

“…However, controversies still exist in the classification and discrimination of Epimedium species. On the one hand, phylogenetic research based on molecular markers [2], flavonoid types [3], and amplified fragment length polymorphisms (AFLP) [4] could not support Stearn’s classification system. On the other hand, the majority of Epimedium species are easily confused with one another due to their highly similar morphology, thereby complicating the distinction between these species.…”

Section: Introductionmentioning

confidence: 99%

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Development of Plastid Genomic Resources for Discrimination and Classification of Epimedium wushanense (Berberidaceae)

Guo

Ren

et al. 2019

IJMS

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Epimedium wushanense (Berberidaceae) is recorded as the source plant of Epimedii Wushanensis Folium in the Chinese Pharmacopoeia. However, controversies exist on the classification of E. wushanense and its closely related species, namely, E. pseudowushanense, E. chlorandrum, E. mikinorii, E. ilicifolium, and E. borealiguizhouense. These species are often confused with one another because of their highly similar morphological characteristics. This confusion leads to misuse in the medicinal market threatening efficiency and safety. Here, we studied the plastid genomes of these Epimedium species. Results show that the plastid genomes of E. wushanense and its relative species are typical circular tetramerous structure, with lengths of 156,855–158,251 bp. A total of 112 genes were identified from the Epimedium plastid genomes, including 78 protein-coding, 30 tRNA, and 4 rRNA genes. A loss of rpl32 gene in E. chlorandrum was found for the first time in this study. The phylogenetic trees constructed indicated that E. wushanense can be distinguished from its closely related species. E. wushanense shows a closer relationship to species in ser. Dolichocerae. In conclusion, the use of plastid genomes contributes useful genetic information for identifying medicinally important species E. wushanense and provides new evidence for understanding phylogenetic relationships within the Epimedium genus.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Plastid Genomic Resources for Discrimination and Classification of Epimedium wushanense (Berberidaceae)

Guo

Ren

et al. 2019

IJMS

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“…A number of taxonomic controversies exist in the Chinese sect. Diphyllon [18,19,48], and efficient methods for species identification and classification are lacking. In our study, the commonly used DNA barcodes were effective in identifying 17 Epimedium species, but the phylogenetic relationships of Chinese sect.…”

Section: Discussionmentioning

confidence: 99%

“…Chinese sect. Diphyllon reaches the highest species diversity with more than 80% species in Epimedium [17], presenting numerous taxonomic controversies [18,19,20,21]. Morphological classification experts identify and publish new species according to their leaf and corolla characteristics [12].…”

Section: Introductionmentioning

confidence: 99%

A Systematic Study on DNA Barcoding of Medicinally Important Genus Epimedium L. (Berberidaceae)

Guo

Ren

et al. 2018

Genes

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Genus Epimedium consists of approximately 50 species in China, and more than half of them possess medicinal properties. The high similarity of species’ morphological characteristics complicates the identification accuracy, leading to potential risks in herbal efficacy and medical safety. In this study, we tested the applicability of four single loci, namely, rbcL, psbA-trnH, internal transcribed spacer (ITS), and ITS2, and their combinations as DNA barcodes to identify 37 Epimedium species on the basis of the analyses, including the success rates of PCR amplifications and sequencing, specific genetic divergence, distance-based method, and character-based method. Among them, character-based method showed the best applicability for identifying Epimedium species. As for the DNA barcodes, psbA-trnH showed the best performance among the four single loci with nine species being correctly differentiated. Moreover, psbA-trnH + ITS and psbA-trnH + ITS + rbcL exhibited the highest identification ability among all the multilocus combinations, and 17 species, of which 12 are medicinally used, could be efficiently discriminated. The DNA barcode data set developed in our study contributes valuable information to Chinese resources of Epimedium. It provides a new means for discrimination of the species within this medicinally important genus, thus guaranteeing correct and safe usage of Herba Epimedii.

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“…Rhizophyllum and four sections of subg. Epimedium as five distinctive clades (Sun et al, 2005 ; Zhang et al, 2007 , 2014 ; De Smet et al, 2012 ). The two subgenera were not well-supported, the relationships between five clades were unresolved except for sect.…”

Section: Introductionmentioning

confidence: 99%

The Complete Chloroplast Genome Sequences of Five Epimedium Species: Lights into Phylogenetic and Taxonomic Analyses

et al. 2016

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Epimedium L. is a phylogenetically and economically important genus in the family Berberidaceae. We here sequenced the complete chloroplast (cp) genomes of four Epimedium species using Illumina sequencing technology via a combination of de novo and reference-guided assembly, which was also the first comprehensive cp genome analysis on Epimedium combining the cp genome sequence of E. koreanum previously reported. The five Epimedium cp genomes exhibited typical quadripartite and circular structure that was rather conserved in genomic structure and the synteny of gene order. However, these cp genomes presented obvious variations at the boundaries of the four regions because of the expansion and contraction of the inverted repeat (IR) region and the single-copy (SC) boundary regions. The trnQ-UUG duplication occurred in the five Epimedium cp genomes, which was not found in the other basal eudicotyledons. The rapidly evolving cp genome regions were detected among the five cp genomes, as well as the difference of simple sequence repeats (SSR) and repeat sequence were identified. Phylogenetic relationships among the five Epimedium species based on their cp genomes showed accordance with the updated system of the genus on the whole, but reminded that the evolutionary relationships and the divisions of the genus need further investigation applying more evidences. The availability of these cp genomes provided valuable genetic information for accurately identifying species, taxonomy and phylogenetic resolution and evolution of Epimedium, and assist in exploration and utilization of Epimedium plants.

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Additional evidence for recent divergence of Chinese Epimedium (Berberidaceae) derived from AFLP, chloroplast and nuclear data supplemented with characterisation of leaflet pubescence

Cited by 20 publications

References 0 publications

Development of Plastid Genomic Resources for Discrimination and Classification of Epimedium wushanense (Berberidaceae)

Development of Plastid Genomic Resources for Discrimination and Classification of Epimedium wushanense (Berberidaceae)

A Systematic Study on DNA Barcoding of Medicinally Important Genus Epimedium L. (Berberidaceae)

The Complete Chloroplast Genome Sequences of Five Epimedium Species: Lights into Phylogenetic and Taxonomic Analyses

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