Additional deleterious effects of alcohol consumption on sperm parameters and DNA integrity in diabetic mice

Pourentezari, Majid; Talebi, Ali Reza; Mangoli, Esmat; Anvari, Morteza; Rahimipour, Marzieh

doi:10.1111/and.12481

Cited by 27 publications

(28 citation statements)

References 17 publications

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“…Thereafter, it was stained for 10 min in freshly prepared acridine orange (0.19 mg/mL in Mcllvain phosphate–citrate buffer, pH 4.0). The smear was then examined under a fluorescent microscope (Olympus BX41) with a 460‐nm filter (Pourentezari et al ., ). For each rat, two slides were stained, and the number of spermatozoa with fragmented nDNA (orange–red fluorescence) was counted in 100 spermatozoa/field.…”

Section: Methodsmentioning

confidence: 97%

Down‐regulation of steroidogenesis‐related genes and its accompanying fertility decline in streptozotocin‐induced diabetic male rats: ameliorative effect of metformin

et al. 2018

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Background: Metformin has long been used for glycemic control in diabetic state. Recently, other benefits of metformin beyond blood glucose regulation have emerged. Objectives: To investigate the effect of metformin on the expression of testicular steroidogenesis-related genes, spermatogenesis, and fertility of male diabetic rats. Materials and methods: Eighteen adult male Sprague Dawley rats were divided into three groups, namely normal control (NC), diabetic control (DC), and metformin-treated (300 mg/kg body weight/day) diabetic rats (D+Met). Diabetes was induced using a single intraperitoneal injection of streptozotocin (60 mg/kg b.w.), followed by oral treatment with metformin for four weeks. Results: Diabetes decreased serum and intratesticular testosterone levels and increased serum but not intratesticular levels of luteinizing hormone. Sperm count, motility, viability, and normal morphology were decreased, while sperm nuclear DNA fragmentation was increased in DC group, relative to NC group. Testicular mRNA levels of androgen receptor, luteinizing hormone receptor, cytochrome P450 enzyme (CYP11A1), steroidogenic acute regulatory (StAR) protein, 3b-hydroxysteroid dehydrogenase (HSD), and 17b-HSD, as well as the level of StAR protein and activities of CYP11A1, 3b-HSD, and 17b-HSD, were decreased in DC group. Similarly, decreased activities of epididymal antioxidant enzymes and increased lipid peroxidation were observed in DC group. Consequently, decreased litter size, fetal weight, mating and fertility indices, and increased pre-and post-implantation losses were recorded in DC group. Following intervention with metformin, we observed increases in serum and intratesticular testosterone levels, Leydig cell count, improved sperm parameters, and decreased sperm nuclear DNA fragmentation. Furthermore, mRNA levels and activities of steroidogenesis-related enzymes were increased, with improved fertility outcome. Discussion and conclusion: Diabetes mellitus is associated with dysregulation of steroidogenesis, abnormal spermatogenesis, and fertility decline. Controlling hyperglycemia is therefore crucial in preserving male reproductive function. Metformin not only regulates blood glucose level, but also preserves male fertility in diabetic state. Research has shown that DM impairs several aspects of the male reproductive function (Nna et al., 2017a). Decreased daily sperm production, sperm count, motility and viability, increased 110

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Section: Methodsmentioning

confidence: 97%

Down‐regulation of steroidogenesis‐related genes and its accompanying fertility decline in streptozotocin‐induced diabetic male rats: ameliorative effect of metformin

et al. 2018

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“…Diabetes mellitus (DM) DM is one of the most common diseases that threats human health in the modern world ( 17 ). Its effects on human health are mostly due to the hyperglycaemic and hypoinsulinemic states caused by the disease, usually affecting neurological, endocrinological, and reproductive functions ( 18 - 20 ). DM has been related to the sexual dysfunction, both in men and women.…”

Section: Introductionmentioning

confidence: 99%

“…Usually, the concentration of seminal insulin is higher than that in the serum. Numerous studies have demonstrated a marked reduction in sperm quality in diabetic male human and animals ( 20 , 24 ). Mangoli et al showed that DM had negative effects on the sperm parameters and chromatin features in mice ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

The etiologies of sperm DNA abnormalities in male infertility: An assessment and review

Pourmasumi¹,

Sabeti²,

Rahiminia³

et al. 2017

IJRM

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The sperm DNA damage may occur in testis, genital ducts, and also after ejaculation. Mechanisms altering chromatin remodeling are abortive apoptosis and oxidative stress resulting from reactive oxygen species. Three classifications of intratesticular, post-testicular, and external factors have been correlated with increased levels of human sperm DNA damage which can affect the potential of fertility. Lifestyle, environment, medical, and iatrogenic factors might be considered to cause dysmetabolism to make distracting interactions and endocrine disrupting compounds. As a result, these may induce chromatin/DNA alteration in germ cells, which may be transmitted across generations with phenotypic consequences. Alcohol consumption may not increase the rate of sperm residual histones and protamine deficiency; however, it causes an increase in the percentage of spermatozoa with DNA fragmentation and apoptosis. In a medical problem as spinal cord injury, poor semen parameters and sperm DNA damage were reported. Infection induces reactive oxygen species production, decreases the total antioxidant capacity and sperm DNA fragmentation or antigen production that lead to sperm dysfunctions and DNA fragmentation. While reactive oxygen species generation increases with age, oxidative stress may be responsible for the age-dependent sperm DNA damage. The exposing of reproductive organs in older men to oxidative stress for a long time may produce more DNA-damaged spermatozoa than youngers. Examining the sperm chromatin quality in testicular cancer and Hodgkin’s lymphoma patients prior to chemotherapy demonstrated the high incidence of DNA damage and low compaction in spermatozoa at the time of the diagnosis. In chemotherapy cycle with genotoxic agents in cancer patients, an increase in sperm DNA damage was shown after treatment. In overall, those factors occurring during the prenatal or the adult life alter the distribution of proteins associated with sperm chromatin induce changes in germ cells which can be detected in infertile patients.

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“…Apart from alcohol, chronic diseases like diabetes has been associated with infertility too. Another experimental study that involved ethanol consumption in diabetes animal model has been established (Pourentezari et al, 2016). This study used male mice with streptozotocin (200 mg/kg, single dose, intraperitoneal) for diabetes induction.…”

Section: Preclinical Evidencementioning

confidence: 99%

Effect of Alcohol Consumption on the Sperm DNA Integrity: A Systematic Review

Jaafar¹,

Osman²,

Kumar³

et al. 2017

JAS

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There is no solid conclusion on the conventional sperm parameters in association with alcohol consumption, evaluation of sperm DNA integrity thus become a more reliable parameter. Hereby, this literature search was performed to summarize alcohol consumption on the sperm DNA integrity. A computerized database search was done through MEDLINE via Ovid (since 1946 until August 2017) and Cochrane was used. The following set of keywords: 'alcohol consumption OR alcohol intake OR alcohol diet OR drinking alcohol OR ethanol diet' AND 'sperm DNA OR sperm chromatin OR sperm genome OR sperm histone OR sperm protamine' were utilised. 24 articles were retrieved where only five studies conform to the inclusion criteria All studies demonstrated a negative effect of alcohol consumption on sperm DNA integrity, regardless of various range of alcohol doses and duration of alcohol consumption. Out of five studies reviewed, four studies were using a different approach to measure the sperm DNA damage. Hereby, this review identified a need to use a single approach of DNA damage test by having various method of alcohol administration and/or vice versa so that the extension of sperm DNA damage to alcohol consumption will have a better conclusion. On the same note, a few studies have reported the reversibility on conventional semen parameters, none has been done on the sperm DNA damage upon alcohol withdrawal. Therefore, the role of alcohol withdrawal on the reversibility of sperm DNA damage needs to be as well investigated further.

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Additional deleterious effects of alcohol consumption on sperm parameters and DNA integrity in diabetic mice

Cited by 27 publications

References 17 publications

Down‐regulation of steroidogenesis‐related genes and its accompanying fertility decline in streptozotocin‐induced diabetic male rats: ameliorative effect of metformin

Down‐regulation of steroidogenesis‐related genes and its accompanying fertility decline in streptozotocin‐induced diabetic male rats: ameliorative effect of metformin

The etiologies of sperm DNA abnormalities in male infertility: An assessment and review

Effect of Alcohol Consumption on the Sperm DNA Integrity: A Systematic Review

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