Addition of β-mercaptoethanol or Trolox® at the morula/blastocyst stage improves the quality of bovine blastocysts and prevents induction of apoptosis and degeneration by prooxidant agents

Feugang, Jean M.; Roover, R. De; Moens, André; Leonard, Samuel L.; Dessy, F.; Donnay, Isabelle

doi:10.1016/s0093-691x(03)00191-2

Cited by 73 publications

(55 citation statements)

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“…In contrast, Geshi et al [16] showed that 10 µM βME in a co-culture system improves embryo development and Cammano et al [15] did not observed any difference between a low (10 µM) or high (100 µM) concentration of βME in development of resulting embryos. In addition, Geshi et al [16], Caamano et al [14] and Feuagang et al [23] reported better promoting effect of βME when added at 4-8 cell, 8-16 cell and beyond the morula stages, respectively. Therefore, it seems that the apparent differences among studies could be attributed to the culture conditions or developmental stage in which βME has been added.…”

Section: Discussionmentioning

confidence: 97%

“…Following cryopreservation, oocytes and embryos become especially more sensitive to the oxidative stress, resulting in lipid peroxidation, membrane injury and structural destruction [3,23,24]. Low molecular-weight thiol compounds, such as βME, maintain the redox state of cells and protect them against the harmful effects of oxidative injuries and a number of studies have indicated the promoting effects of βME during in vitro embryo production [14][15][16][17][18].…”

Section: Discussionmentioning

confidence: 99%

“…However, data on the effect of βME are conflicting or inconsistent regarding favorable concentration of βME used in culture medium, and regarding the stage of IVP in which βME supplementation better supports embryo development. For example, Feugang et al [23] demonstrated no protective effect of βME on embryo development when added at 50 µM. In contrast, Geshi et al [16] showed that 10 µM βME in a co-culture system improves embryo development and Cammano et al [15] did not observed any difference between a low (10 µM) or high (100 µM) concentration of βME in development of resulting embryos.…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Hosseini

Forouzanfar

Hajian

et al. 2009

J Assist Reprod Genet

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Purpose To determine the most optimal stage for antioxidant supplementation of culture medium to improve developmental competence, cryotolerance and DNAfragmentation of bovine embryos. Methods Presumptive zygotes were first cultured in presence or absence of β-mercaptoethanol (β-ME), for 8 days. Subsequently, half of the expanded blastocysts developed in both groups were vitrified, warmed within 30 min and post-warming embryos along with their corresponding non-vitrified embryos were cultured for two further days in presence or absence of (100 µM) βME. Results For vitrified and non-vitrified embryos, the best effect was found when βME was added from day 1 of in vitro culture in continuation with post-warming culture period. Day 1-8 supplementation significantly increased the rates of cleavage, day 7 and day 8 blastocyst production. For non-vitrified embryos, βME addition during day 1-8 and/or 9-10 of embryo culture improved both hatching rate and quality of hatched embryos. For vitrified embryos, however, the percentage of DNA-fragmentation (18.5%) was significantly higher (p≤0.05) than that of embryos developed in absence of βME but supplemented with βME during post-warming period (13.5%). Conclusions Exogenous antioxidant increases the chance of embryos, even those of fair-quality, to develop to blastocyst. However, antioxidant inclusion during in vitro embryo development is not sufficient to maintain the redox state of these embryos during the critical period of post-warming embryo culture, and therefore, there should be a surplus source of exogenous antioxidant during post-warming embryo culture.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Hosseini

Forouzanfar

Hajian

et al. 2009

J Assist Reprod Genet

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“…However, increasing concentrations of vitamin E are toxic to bovine embryos as also observed in mouse embryos (Wang et al, 2002). Indeed, concentrations above 400 mM induced a dose-dependent decrease in blastocyst development and blastocyst cell number (Feugang et al, 2004). In conclusion, buffalo spermatozoa treated with a combination of heparin, caffeine and calcium ionophore failed to increase embryo development compared to heparin alone.…”

Section: Discussionmentioning

confidence: 72%

“…Recently, vitamin E (Trolox R , water-soluble analog) added to culture medium was shown to decrease the H 2 O 2 content and increase the developmental ability to the blastocyst stage and the cell number in porcine IVF embryos (Kitagawa et al, 2004). Moreover, Feugang et al (2004) reported that vitamin E could protect bovine morulae/blastocysts exposed to oxidative stress generated through an increase in ROS production or a decrease in antioxidant protection. However, increasing concentrations of vitamin E are toxic to bovine embryos as also observed in mouse embryos (Wang et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

α-Tocopherol and l-ascorbic acid increase the in vitro development of IVM/IVF swamp buffalo (Bubalus bubalis) embryos

Saikhun

Faisaikarm

Zhang

et al. 2008

Animal

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This study was conducted to investigate the effects of capacitating agents added at in vitro fertilization (IVF) and antioxidants supplemented during in vitro culture (IVC) on the development of buffalo embryos. In experiment I, in vitro embryo development of buffalo embryos was compared when the IVF medium was supplemented with heparin, caffeine and calcium ionophore A23187 either alone or in combination. There was no significant difference (P . 0.05) in the cleavage rates of oocytes among the treatment groups but the development rate to the blastocyst stage and the cell numbers of blastocyst in the heparin-treated group were significantly higher (P , 0.05) than that of other treatments. In experiment II, in vitro embryo development of buffalo embryos was compared when IVC medium was supplemented with either a-tocopherol (250 and 500 mM) or L-ascorbic acid (250 and 500 mM). The rate of development to the blastocyst stage of embryos cultured in medium supplemented with 250 mM a-tocopherol (33%, 41/123) and 250 mM L-ascorbic acid (31%, 38/123) was significantly higher (P , 0.05) than that of those cultured in medium alone (19%, 20/108) but not significantly different (P , 0.05) from medium supplemented with either 500 mM a-tocopherol (24%, 30/123) or 500 mM L-ascorbic acid (25%, 33/133). These results suggest that buffalo spermatozoa treated with heparin were suitable for IVF and that a-tocopherol and L-ascorbic acid added during IVC increased the rate of buffalo embryo development.Keywords: buffalo, embryo, development, a-tocopherol, L-ascorbic acid IntroductionSince the birth of the first buffalo calf from an in vitro fertilized (IVF) oocyte (Madan et al., 1991), a number of articles on in vitro embryo production (IVP) have been published. However, success, in terms of production of transferable stage embryos and birth of calves following embryo transfer, has been limited (Gasparrini, 2002;Nandi et al., 2002). Moreover, the efficiency of IVP in buffalo is much lower than that in cattle (Nandi et al., 2002;Gasparrini, 2002). IVP in cattle can be considered to be a mature technology and available for commercialization (van Wagtendonk-de Leeuw, 2006); on the other hand, IVP systems in buffalo are suboptimal and require substantial improvements (Nandi et al., 2002;Gasparrini, 2002). Several factors might affect the IVP in this species, such as inadequate oocyte maturation, an inappropriate timing of insemination and a non-optimal time of gamete co-incubation. Moreover, the important factors that might affect embryo development are the chemical agents used for capacitation of spermatozoa during IVF and the oxidative damage of embryos during in vitro culture (IVC). Several agents have been used to induce capacitation of buffalo spermatozoa. Heparin is structurally similar to glycosaminoglycans, which were shown to induce capacitation and acrosome reaction (AR) in bovine spermatozoa (Parrish et al., 1988) and several mammalian species including buffalo (Chauhan et al., 1997;Kitiyanant et al., 2002). Caffeine stimulate...

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Elevated p66Shc is associated with intracellular redox imbalance in developmentally compromised bovine embryos

Bain

Madan

Betts

2012

Molecular Reproduction Devel

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The in vitro production of mammalian embryos suffers from low efficiency, with 50-70% of all fertilized oocytes failing to develop to the blastocyst stage. This high rate of developmental failure is due, in part, to the effects of oxidative stress generated by reactive oxygen species (ROS). The p66Shc adaptor protein controls oxidative stress response by regulating intracellular ROS levels through multiple pathways, including mitochondrial ROS generation and the repression of antioxidants. This study explored the relationship between p66Shc levels, redox state, and developmental potential in early bovine embryos. Embryo developmental potential was established based on observing their time of first cleavage. P66Shc, catalase, and mitochondrial-specific, manganese-superoxide dismutate (MnSOD) levels were compared between embryos with high and low developmental potentials. Additionally, p66Shc, catalase, and MnSOD content were assayed following a variety of oxidative stress-inducing and-alleviating conditions. Increased developmental potential correlated with significantly lower p66Shc content, significantly higher levels of catalase and MnSOD, and significantly lower intracellular ROS levels (MitoSOX staining) and reduced DNA damage (γ-H2A.X(phospho S139) immunostaining). p66Shc content was increased by either high (20%) O(2) culture or H(2)O(2) treatment, and significantly decreased by supplementing culture media with the antioxidant polyethylene glycol-conjugated catalase. While the abundance of p66Shc varied according to pro/anti-oxidant culture conditions, antioxidant content varied only according to developmental potential. This discrepancy has important implications regarding ongoing efforts towards maximizing in vitro embryo production.

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Addition of β-mercaptoethanol or Trolox® at the morula/blastocyst stage improves the quality of bovine blastocysts and prevents induction of apoptosis and degeneration by prooxidant agents

Cited by 73 publications

References 72 publications

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

α-Tocopherol and l-ascorbic acid increase the in vitro development of IVM/IVF swamp buffalo (Bubalus bubalis) embryos

Elevated p66Shc is associated with intracellular redox imbalance in developmentally compromised bovine embryos

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