Addition of Mesenchymal Stem Cells to Autologous Platelet-Enhanced Fibrin Scaffolds in Chondral Defects

Lr, Goodrich; Ac, Chen; Nm, Werpy; Aw, Williams; Jd, Kisiday; Aw, Su; Cory, Esther; Morley, Paul S.; Cw, McIlwraith; Rl, Sah; Chu, Constance R.

doi:10.2106/jbjs.o.00407

Cited by 59 publications

(65 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…29 When MSCs were instead combined with platelet-enhanced fibrin scaffolds in a lateral trochlear ridge defect of the stifle joint, ectopic bone formed in the repair tissue, which resulted in a thinner repair tissue when compared with cell-free scaffolds alone. 44 These findings indicated that MSCs should not be combined with platelet-enhanced fibrin scaffold for cartilage regeneration owing to the risk of bone formation. 44 In contrast, when autologous, culture-expanded chondroprogenitor cells were implanted in fibrin scaffolds, improved cartilage repair tissue was observed when compared with culture-expanded allogeneic chondroprogenitor cells or fibrin scaffold alone.…”

Section: Equine Models Of Focal Cartilage Healingmentioning

confidence: 99%

“…44 These findings indicated that MSCs should not be combined with platelet-enhanced fibrin scaffold for cartilage regeneration owing to the risk of bone formation. 44 In contrast, when autologous, culture-expanded chondroprogenitor cells were implanted in fibrin scaffolds, improved cartilage repair tissue was observed when compared with culture-expanded allogeneic chondroprogenitor cells or fibrin scaffold alone. 45 Although, chondroprogenitor cells may differ significantly from MSCs, the study suggests further investigation into using cells within a scaffold.…”

Section: Equine Models Of Focal Cartilage Healingmentioning

confidence: 99%

See 1 more Smart Citation

Equine Models for the Investigation of Mesenchymal Stem Cell Therapies in Orthopaedic Disease

Colbath

Frisbie

Dow

et al. 2017

Operative Techniques in Sports Medicine

Self Cite

View full text Add to dashboard Cite

Section: Equine Models Of Focal Cartilage Healingmentioning

confidence: 99%

Section: Equine Models Of Focal Cartilage Healingmentioning

confidence: 99%

Equine Models for the Investigation of Mesenchymal Stem Cell Therapies in Orthopaedic Disease

Colbath

Frisbie

Dow

et al. 2017

Operative Techniques in Sports Medicine

Self Cite

View full text Add to dashboard Cite

“…Most of these studies suggest promising results with improved cartilage regeneration between 3 months and 2 years. 26,[32][33][34][35][36] Significant superior histologic evidence of cartilage regeneration when using hydrogels are reported when compared to empty defects. The cell sources, collection techniques, cell processing, qualitative and quantitative characterizations, and delivery methods vary widely between studies.…”

Section: Preclinical Animal Modelsmentioning

confidence: 99%

Current and novel injectable hydrogels to treat focal chondral lesions: Properties and applicability

Pascual‐Garrido

Rodríguez-Fontán

Aisenbrey

et al. 2017

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

Focal chondral lesions and early osteoarthritis (OA) are responsible for progressive joint pain and disability in millions of people worldwide, yet there is currently no surgical joint preservation treatment available to fully restore the long term functionality of cartilage. Limitations of current treatments for cartilage defects have prompted the field of cartilage tissue engineering, which seeks to integrate engineering and biological principles to promote the growth of new cartilage to replace damaged tissue. Toward improving cartilage repair, hydrogel design has advanced in recent years to improve their utility. Injectable hydrogels have emerged as a promising scaffold due to their wide range of properties, the ability to encapsulate cells within the material, and their ability to provide cues for cell differentiation. Some of these advances include the development of improved control over in situ gelation (e.g., light), new techniques to process hydrogels (e.g., multi-layers), and better incorporation of biological signals (e.g., immobilization, controlled release, and tethering). This review summarises the innovative approaches to engineer injectable hydrogels toward cartilage repair. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:64-75, 2018.

show abstract

“…In general the implantation of undifferentiated MSCs has been shown to promote the accumulation of cartilage‐like repair tissue, although this approach does not reliably result in regeneration of articular cartilage . Further, several animal studies have reported cartilage healing with undifferentiated MSC grafts that was no better than acellular controls . Therefore, controlled induction of chondrogenesis appears to be an important aspect of the use of MSCs for cartilage tissue engineering.…”

mentioning

confidence: 99%

“…1 Further, several animal studies have reported cartilage healing with undifferentiated MSC grafts that was no better than acellular controls. [2][3][4] Therefore, controlled induction of chondrogenesis appears to be an important aspect of the use of MSCs for cartilage tissue engineering.…”

mentioning

confidence: 99%

Effect of culture duration on chondrogenic preconditioning of equine bone marrow mesenchymal stem cells in self‐assembling peptide hydrogel

Kisiday

Colbath

Tangtrongsup

2018

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

Ex vivo induction of chondrogenesis is a promising approach to improve upon the use of bone marrow mesenchymal stem cells (MSCs) for cartilage tissue engineering. This study evaluated the potential to induce chondrogenesis with days of culture in chondrogenic medium for MSCs encapsulated in self-assembling peptide hydrogel. To simulate the transition from preconditioning culture to implantation, MSCs were isolated from self-assembling peptide hydrogel into an individual cell suspension. Commitment to chondrogenesis was evaluated by seeding preconditioned MSCs into agarose and culturing in the absence of the chondrogenic cytokine transforming growth factor beta (TGFβ). Positive controls consisted of undifferentiated MSCs seeded into agarose and cultured in medium containing TGFβ. Three days of preconditioning was sufficient to produce chondrogenic MSCs that accumulated ∼75% more cartilaginous extracellular matrix than positive controls by day 17. However, gene expression of type X collagen was ∼65-fold higher than positive controls, which was attributed to the absence of TGFβ. Potential induction of immunogenicity with preconditioning culture was indicated by expression of major histocompatibility complex class II (MHCII), which was nearly absence in undifferentiated MSCs, and ∼7% positive for preconditioned cells. These data demonstrate the potential to generate chondrogenic MSCs with days of self-assembling peptide hydrogel, and the ability to readily recover an individual cell suspension that is suited for injectable therapies. However, continued exposure to TGFβ may be necessary to prevent hypertrophy indicated by type X collagen expression, while immunogenicity may be a concern for allogeneic applications. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.

show abstract

Addition of Mesenchymal Stem Cells to Autologous Platelet-Enhanced Fibrin Scaffolds in Chondral Defects

Abstract: APEF supported repair of critical-size full-thickness chondral defects in horses, which was not improved by the addition of BMDMSCs. This work supports further investigation to determine whether APEF enhances cartilage repair in humans.

Cited by 59 publications

References 50 publications

Equine Models for the Investigation of Mesenchymal Stem Cell Therapies in Orthopaedic Disease

Equine Models for the Investigation of Mesenchymal Stem Cell Therapies in Orthopaedic Disease

Current and novel injectable hydrogels to treat focal chondral lesions: Properties and applicability

Effect of culture duration on chondrogenic preconditioning of equine bone marrow mesenchymal stem cells in self‐assembling peptide hydrogel

Contact Info

Product

Resources

About