Addition of l-carnitine to formulated feed improved growth performance, antioxidant status and lipid metabolism of juvenile largemouth bass, Micropterus salmoides

Chen, Yifang; Sun, Zhenzhu; Liang, Zuman; Xie, Yongdong; Tan, Xiaohong; Su, Jiliang; Luo, Qiulan; Zhu, Junyan; Liu, Qingying; Wang, An‐Li

doi:10.1016/j.aquaculture.2019.734434

Cited by 22 publications

(8 citation statements)

References 44 publications

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“…Each sample was run in duplicate, and the relative expressions were calculated according to Vandesompele et al (36). The primers of qPCR were designed using the National Center for Biotechnology Information (NCBI) primer BLAST service or from the previous studies (37)(38)(39)(40)(41)(42) and shown in Table 2.…”

Section: Sample Collection and Growth Performance Determinationmentioning

confidence: 99%

Dietary berberine alleviates high carbohydrate diet-induced intestinal damages and improves lipid metabolism in largemouth bass (Micropterus salmoides)

Gong

Liu

et al. 2022

Front. Nutr.

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High carbohydrate diet (HCD) causes metabolism disorder and intestinal damages in aquaculture fish. Berberine has been applied to improve obesity, diabetes and NAFLD. However, whether berberine contributes to the alleviation of HCD-induced intestinal damages in aquaculture fish is still unclear. Here we investigated the effects and mechanism of berberine on HCD-induced intestinal damages in largemouth bass (Micropterus salmoides). We found dietary berberine (50 mg/kg) improved the physical indexes (VSI and HSI) without affecting the growth performance and survival rate of largemouth bass. Importantly, the results showed that dietary berberine reduced the HCD-induced tissue damages and repaired the barrier in the intestine of largemouth bass. We observed dietary berberine significantly suppressed HCD-induced intestinal apoptosis rate (from 31.21 to 8.35%) and the activity level of Caspase3/9 (P < 0.05) by alleviating the inflammation (il1β, il8, tgfβ, and IL-6, P < 0.05) and ER stress (atf6, xbp1, perk, eif2α, chopa, chopb, and BIP, P < 0.05) in largemouth bass. Further results showed that dietary berberine declined the HCD-induced excessive lipogenesis (oil red O area, TG content, acaca, fasn, scd, pparγ, and srebp1, P < 0.05) and promoted the lipolysis (hsl, lpl, cpt1a, and cpt2, P < 0.05) via activating adenosine monophosphate-activated protein kinase (AMPK, P < 0.05) and inhibiting sterol regulatory element-binding protein 1 (SREBP1, P < 0.05) in the intestine of largemouth bass. Besides, we also found that dietary berberine significantly promoted the hepatic lipid catabolism (hsl, lpl, cpt1a, and cpt2, P < 0.05) and glycolysis (pk and ira, P < 0.05) to reduce the systematic lipid deposition in largemouth bass fed with HCD. Therefore, we elucidated that 50 mg/kg dietary berberine alleviated HCD-induced intestinal damages and improved AMPK/SREBP1-mediated lipid metabolism in largemouth bass, and evaluated the feasibility for berberine as an aquafeed additive to enhance the intestinal function of aquaculture species.

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Section: Sample Collection and Growth Performance Determinationmentioning

confidence: 99%

Dietary berberine alleviates high carbohydrate diet-induced intestinal damages and improves lipid metabolism in largemouth bass (Micropterus salmoides)

Gong

Liu

et al. 2022

Front. Nutr.

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“…Carnitine was also used in the extender media for rainbow trout sperm cryopreservation and it was able to improve post-thaw motility, although fertility was not affected by the supplement, the higher fertilization rate was obtained with samples cryopreserved with carnitine [ 29 ]. In other marine and freshwater species, such as the European seabass [ 116 ], largemouth bass ( Micropterus salmoides ) [ 117 ] and common carp [ 118 ], L-carnitine proved to have an impact on the growth performance and antioxidant status. Further studies are necessary to better comprehend the mechanisms of action of this compound, specially at fish spermatozoa level.…”

Section: Fish Sperm Antioxidant System and Supplementation: Classimentioning

confidence: 99%

Antioxidants in Fish Sperm and the Potential Role of Melatonin

2020

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In recent years, the effects of novel antioxidants have played an important role in the research focusing on fish cell protection. As food demand grows, aquaculture production becomes more intensive, and fish are more exposed to oxidative stress conditions, like high densities, temperature shifting, frequent fish handling and samplings, and prophylactic or disease treatments, which expose fish to a different environment. Particularly in reproduction, germ cells lose antioxidant capacity with spermatogenesis, as spermatozoa are more prone to oxidative stress. Antioxidants have been used in a variety of fish physiological problems including in reproduction and in the establishment of cryopreservation protocols. From the most used antioxidants to natural plant food and herbs, and endogenously produced antioxidants, like melatonin, a review of the literature available in terms of their effects on the protection of fish spermatozoa is presented here in a classified structure. Several direct and indirect approaches to improve gamete quality using antioxidants administration are mentioned (through feed supplementation or by adding in cryopreservation media), as well as factors affecting the efficiency of these molecules and their mechanisms of action. Special attention is given to the unclear melatonin pathway and its potential scavenger activity to prevent and counteract oxidative stress damage on fish spermatozoa.

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“…Progress is urgently needed to find effective additives to reduce inflammation and abnormal lipid deposition in fish fed the diet with a high level of TO. L-Carnitine plays a crucial role in shuttling long-chain fatty acids into mitochondria (21) and reduces abnormal lipid deposition (22,23) . L-Carnitine can also increase the immunocompetence of several fish species to better cope with biotic and abiotic stresses (24)(25)(26) .…”

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confidence: 99%

Effects of dietary terrestrial oils supplemented with l-carnitine on growth, antioxidant capacity, lipid metabolism and inflammation in large yellow croaker (Larimichthys crocea)

Chen

et al. 2020

Br J Nutr

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The present study was conducted to determine the effects of dietary terrestrial oils (TO) supplemented with L-carnitine on growth performance, biochemical and antioxidant response, lipid metabolism and inflammation in large yellow croaker (Larimichthys crocea). Three iso-nitrogenous and iso-lipidic experimental diets were formulated with FO (fish oil, the control group), 75% TO (75% FO was substituted by the oil mixture with equal amounts of soybean oil, linseed oil and pork lard) and 75% TOC (75% TO supplemented with 800 mg/kg L-carnitine), respectively. Compared to the control group, feed efficiency ratio and specific growth rate were significantly increased in fish fed diets with 75% TO and 75% TOC. Hepatic lipid content, serum triglyceride level, low-density lipoprotein-cholesterol level and the mRNA expression of pro-inflammatory genes (tnfα and ifnγ) were significantly increased in fish fed the diet with 75% TO compared to the control group. However, the supplementation of 800 mg/kg L-carnitine in the 75% TO diet repressed hepatic lipid content, serum low-density lipoprotein-cholesterol level and the mRNA expression of tnfα and ifnγ in fish compared to fish fed the diet with 75% TO. Total antioxidant capacity, the activity of superoxide dismutase, the mRNA expression of cpt-I and the activity of CPT-I were significantly increased in fish fed the diet with 75% TOC compared to 75% TO. In conclusion, these results suggested that the supplementation of 800 mg/kg L-carnitine in the diet with terrestrial oils mixture could increase growth, antioxidant capacity, fatty acid oxidation and decrease the expression of inflammatory genes in large yellow croaker.

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Addition of l-carnitine to formulated feed improved growth performance, antioxidant status and lipid metabolism of juvenile largemouth bass, Micropterus salmoides

Cited by 22 publications

References 44 publications

Dietary berberine alleviates high carbohydrate diet-induced intestinal damages and improves lipid metabolism in largemouth bass (Micropterus salmoides)

Dietary berberine alleviates high carbohydrate diet-induced intestinal damages and improves lipid metabolism in largemouth bass (Micropterus salmoides)

Antioxidants in Fish Sperm and the Potential Role of Melatonin

Effects of dietary terrestrial oils supplemented with l-carnitine on growth, antioxidant capacity, lipid metabolism and inflammation in large yellow croaker (Larimichthys crocea)

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