Addition of humic acid accelerates the growth of Euglena pisciformis AEW501 and the accumulation of lipids

Fan, Panpan; Xu, Pingping; Zhu, Yuxuan; Tu, Xiaojie; Song, Gaofei; Zuo, Yuegang; Bi, Yonghong

doi:10.1007/s10811-021-02623-9

Cited by 9 publications

(7 citation statements)

References 61 publications

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“…E. pisciformis AEW501 was obtained from the State Key Laboratory of Freshwater Ecology and Biotechnology in the Institute of Hydrobiology, Chinese Academy of Sciences (Wuhan, China). The inoculum was cultivated in a modified mixotrophic AF-6 medium ( Table S1, Supporting Information ) at 20 °C, with the constant light intensity of 50 μmol photons m −2 ∙s −1 using cool white fluorescent lamps as previously reported [ 25 ]. To prepare the inoculants, a single colony of E. pisciformis AEW501 was inoculated into a 50 mL modified AF-6 medium in a 100 mL Erlenmeyer flask.…”

Section: Methodsmentioning

confidence: 99%

“…The total lipid content and fatty acid composition were determined according to the protocol of Fan et al [ 25 ]. The range of vitamins in E. pisformis cells was determined according to Syad et al [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

“…It was shown that E. pisciformis AEW501 could grow under autotrophic, mixotrophic, and heterotrophic modes. Interestingly, the growth rate of this algae under mixotrophic conditions was higher and the paramylon granules were visible in the cytoplasm [ 25 ]. Therefore, mixotrophic cultivation is trending toward the development of new Euglena species to increase both biomass and paramylon production.…”

Section: Introductionmentioning

confidence: 99%

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Mixotrophic Cultivation Optimization of Microalga Euglena pisciformis AEW501 for Paramylon Production

Fan

Deng

et al. 2022

Marine Drugs

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Euglena, a flagellated unicellular protist, has recently received widespread attention for various high-value metabolites, especially paramylon, which was only found in Euglenophyta. The limited species and low biomass of Euglena has impeded paramylon exploitation and utilization. This study established an optimal cultivation method of Euglena pisciformis AEW501 for paramylon production under mixotrophic cultivation. The results showed that the optimum mixotrophic conditions were 20 °C, pH 7.0, and 63 μmol photons m−2∙s−1, and the concentrations of sodium acetate and diammonium hydrogen phosphate were 0.98 g L−1 and 0.79 g L−1, respectively. The maximal biomass and paramylon content were 0.72 g L−1 and 71.39% of dry weight. The algal powder contained more than 16 amino acids, 6 vitamins, and 10 unsaturated fatty acids under the optimal cultivation. E. pisciformis paramylon was pure β-1,3-glucan-type polysaccharide (the purity was up to 99.13 ± 0.61%) composed of linear glucose chains linked together by β-1,3-glycosidic bonds. These findings present a valuable basis for the industrial exploitation of paramylon with E. pisciformis AEW501.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mixotrophic Cultivation Optimization of Microalga Euglena pisciformis AEW501 for Paramylon Production

Fan

Deng

et al. 2022

Marine Drugs

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“…Humic substances are well recognized to aid natural plant growth as a result of the occurrence of rich bioactive organic carbon sources. Therefore, the activity of numerous antioxidant enzymes can be effectively improved to promote the physiological metabolisms of plant cells [24]. Studies carried out by Fan [24] and Zheng [25] observed that the supplementation of humic acid alleviated the growth of Euglena pisciformis AEW501 and Scenedesmus Capricornus, respectively.…”

Section: Nutrient Recovery From Different Extraction Methodsmentioning

confidence: 99%

“…Therefore, the activity of numerous antioxidant enzymes can be effectively improved to promote the physiological metabolisms of plant cells [24]. Studies carried out by Fan [24] and Zheng [25] observed that the supplementation of humic acid alleviated the growth of Euglena pisciformis AEW501 and Scenedesmus Capricornus, respectively. When the culture medium was improved with 50 mg/L humic acids, maximum biomass productivity of 35 mg/L/d and greatest high-value product (lipid content) accumulation of 60% were achieved with Euglena pisciformis AEW501 [24].…”

Section: Nutrient Recovery From Different Extraction Methodsmentioning

confidence: 99%

Malaysian Virgin Soil Extracts as Natural Growth Enhancer for Targeted Green Microalgae Species

et al. 2022

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The microalgae-based industries are trending upwards, particularly as the feed ingredient for aquaculture. Therefore, a sustainable and reasonably priced source of nutrients to support the mass cultivation of microalgae is in great demand. The present study explored the feasibility of using extracts from virgin soil as natural growth-promoting nutrients for the cultivation of Nannochloropsis oculata, Nannochloropsis oceanica, and Chlorella sorokiniana. The extracts were obtained from Bera Lake Forest using five different treatment methods. The greatest retrieval of dissolved organic carbon, total dissolved nitrogen, and total dissolved phosphorus were observed with the autoclave treatment method at 121 °C twice, yielding a respective concentration of 336.56 mg/L, 13.40 mg/L, and 0.14 mg/L, respectively. The highest growth was recorded with Nannochloropsis oculata resulting in an optical density of 0.488 ± 0.009 (×103 cell mL−1), exhibiting 43% and 44% enhanced growth in comparison to Nannochloropsis oceanica and Chlorella sorokiniana, respectively. The specific growth rate (0.114 a ± 0.007 d−1) was the highest for Nannochloropsis oculata when the 24 h-extraction method was used, whereas the utilization of the autoclave 121 °C twice treatment method contributed to the highest specific growth of Nannochloropsis ocenica (0.069 a ± 0.003 d−1) and Chlorella sorokiniana (0.080 a ± 0.001 d−1). Collectively, these findings suggested that the addition of soil extracts which is sustainable and inexpensive promoted the growth of microalgae compared to the control system. A further study investigating the optimum culture conditions for enhanced microalgae growth will be carried out for the mass production of microalgae biomass.

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Exogenous ethanol induces cell giantism accompanied by enhanced accumulation of lipid and carbohydrates in Chlorella sorokiniana

Abate,

Bi,

Song

et al. 2024

J Appl Phycol

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Addition of humic acid accelerates the growth of Euglena pisciformis AEW501 and the accumulation of lipids

Cited by 9 publications

References 61 publications

Mixotrophic Cultivation Optimization of Microalga Euglena pisciformis AEW501 for Paramylon Production

Mixotrophic Cultivation Optimization of Microalga Euglena pisciformis AEW501 for Paramylon Production

Malaysian Virgin Soil Extracts as Natural Growth Enhancer for Targeted Green Microalgae Species

Exogenous ethanol induces cell giantism accompanied by enhanced accumulation of lipid and carbohydrates in Chlorella sorokiniana

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