Addition of carbon sources and nutrient salts can inhibit gangue acidification by changing microbial community structure

Zhu, Quanmin; Ruan, Mengying; Hu, Zhenqi; Yuan, Chun

doi:10.1007/s11356-022-21726-5

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…However, the results of an experiment by Zhu [13] showed that Thiobacillus was not the dominant genus in the microbial community during the early stages of gangue oxidation and that other reasons might exist for gangue acidification. In this study, when the pH of the leaching solution of the newly produced gangue was 4.65, the relative abundance of Thiobacillus spp.…”

Section: Discussionmentioning

confidence: 97%

“…They proliferated rapidly during the second week of the experiment and disappeared after the third week. This may be related to changes in the concentration of moisture, organic matter, and inorganic salts in the gangue [13,23]. Yoon et al [24] suggested that the increase in the structural richness and diversity of the soil bacterial community was also beneficial for inhibiting the proliferation of pathogenic bacteria such as Listeria.…”

Section: Microbial Community Structure Of Coal Ganguementioning

confidence: 99%

“…Therefore, it is especially crucial to take prompt measures to inhibit the growth of acidophilic bacteria in newly generated coal gangue. An important period in the acidification of gangue is within 30 d after it is produced, where it undergoes a neutral to acidic conversion [13]. At this stage, the microbial community on the surface of coal gangue also undergoes significant changes.…”

Section: Introductionmentioning

confidence: 99%

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The Relationship between Acid Production and the Microbial Community of Newly Produced Coal Gangue in the Early Oxidation Stage

Zhu,

Ruan,

et al. 2023

Microorganisms

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Coal gangue is a solid waste formed during coal production, and the acid mine drainage it generates during open-pit storage severely pollutes the ecological environment of mining areas. Microorganisms play a crucial catalytic role in acidification, and their species and gene functions change during the oxidation process of coal gangue. In this study, the changes in microbial community structure were investigated during the initial acidification process for newly produced gangue exposed to moisture by monitoring the changes in pH, EC, sulfate ion concentration, and the iron oxidation rate of gangue leaching solutions. Moreover, the composition and functional abundance of microbial communities on the surface of the gangue were analyzed with rainfall simulation experiments and 16S rRNA sequencing. The study yielded the following findings: (1) The critical period for newly produced gangue oxidation spanned from 0~15 d after its exposure to water; the pH of leaching solutions decreased from 4.65 to 4.09 during this time, and the concentration and oxidation rate of iron in the leaching solutions remained at low levels, indicating that iron oxidation was not the main driver for acidification during this stage. (2) When the gangue was kept dry, Burkholderia spp. dominated the gangue microbial community. When the gangue was exposed to moisture, the rate of acidification accelerated, and Pseudomonas replaced Burkholderia as the dominant genus in the community. (3) In terms of gene function, the microbial community of the acidified gangue had stronger nitrogen cycling functions, and an increase in the abundance of microorganisms related to the sulfur cycle occurred after day 15 of the experiment. The microbial community in the acidified gangue had more stress resistance than the community of the newly formed gangue, but its potential to decompose environmental pollutants decreased.

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Section: Discussionmentioning

confidence: 97%