Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements

Antonijevic, Djordje; Jeschke, Anke; Čolović, Božana; Milovanović, Petar; Jevremović, Danimir; Kisić, Danilo; Scheidt, Annika vom; Hahn, Michael; Amling, Michael; Jokanovic, Vukoman; Busse, Björn; Djurić, Marija

doi:10.1016/j.joen.2015.09.008

Cited by 21 publications

(19 citation statements)

References 28 publications

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“…), bone marrow mesenchymal stem cells showed good proliferation after seeding on this material (Antonijevic et al . ), comet assays on human lymphocytes was associated with low genotoxic potential of this cement (Opačić‐Galić et al . ) and the study of subcutaneous implantation in rats and rabbits revealed high tolerance to the implanted material (Petrović et al .…”

Section: Introductionmentioning

confidence: 99%

“…New formulations of CS and CA cements have been developed by innovative methods of synthesis based on nanotechnology which overcomes these disadvantages (Jokanovi c et al 2014). CS cements have already been investigated in many in vitro and in vivo studies: MTT assays have revealed increased metabolic activity of L929 and MRC-5 cells (Petrovi c et al 2015, Cetenovic et al 2017, bone marrow mesenchymal stem cells showed good proliferation after seeding on this material (Antonijevic et al 2015), comet assays on human lymphocytes was associated with low genotoxic potential of this cement (Opa ci c-Gali c et al 2013) and the study of subcutaneous implantation in rats and rabbits revealed high tolerance to the implanted material (Petrovi c et al 2015, Opa ci c-Gali c et al 2017. Although these materials have good biological behaviour, which suggests their use for clinical application, their biocompatibility has to be further assessed in order to investigate all aspects of their biocompatibility (defined by the international standard ISO 10993: Biological evaluation of medical devices).…”

Section: Introductionmentioning

confidence: 99%

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Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

et al. 2019

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Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty‐four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t‐test) and nonparametric tests (Kruskal–Wallis and Mann–Whitney U‐test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

et al. 2019

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“…The use of YbF 3 in the concentration of 30% was sufficient to promote proper radiopacity, without changing the properties of experimental cement (9). The association of PC with YbF 3 demonstrated radiopacity higher than 4 mm/Al and an increase in the compressive strength of the material (10).…”

Section: Introductionmentioning

confidence: 93%

Ytterbium Oxide as Radiopacifier of Calcium Silicate-Based Cements. Physicochemical and Biological Properties

et al. 2018

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This study evaluated physicochemical properties, cytotoxicity and bioactivity of MTA Angelus (MTA), calcium silicate-based cement (CSC) and CSC with 30% Ytterbium oxide (CSC/Yb2O3). Setting time was evaluated using Gilmore needles. Compressive strength was evaluated in a mechanical machine. Radiopacity was evaluated using radiographs of materials and an aluminum scale. Solubility was evaluated after immersion in water. Cell viability was evaluated by means of MTT assay and neutral red staining, and the mineralization activity by using alkaline phosphatase activity and Alizarin Red staining. The data were submitted to ANOVA, Tukey and Bonferroni tests (5% significance). The bioactive potential was evaluated by scanning electron microscopy. The materials presented similar setting time. MTA showed the lowest compressive strength. MTA and CSC/Yb2O3 presented similar radiopacity. CSC/Yb2O3 showed low solubility. Saos-2 cell viability tests showed no cytotoxic effect, except to 1:1 dilution in NR assay which had lower cell viability when compared to the control. ALP at 1 and 7 days was similar to the control. MTA and CSC had greater ALP activity at 3 days when compared to control. All the materials present higher mineralized nodules when compared with the control. SEM analysis showed structures suggesting the presence of calcium phosphate on the surface of materials demonstrating bioactivity. Ytterbium oxide proved to be a properly radiopacifying agent for calcium silicate-based cement since it did not affected the physicochemical and biological properties besides preserving the bioactive potential of this material.

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“…Although there are some attepts to use different radiopacifier, like ytterbium trifluoride 18 and zirconium oxide 19 that may improve some other properties, we decided to use Bi 2 O 3 because they provide lower contrast. The same radiocontrast agent was used in the case of MTA + .…”

Section: Mtt Testmentioning

confidence: 99%

In vitro biocompatibility of nanostructured endodontic materials using SCAP cells

Ćetenović¹,

Čolović²,

Vasilijić³

et al. 2017

Balkan J Dent Med

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Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements

Cited by 21 publications

References 28 publications

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

Ytterbium Oxide as Radiopacifier of Calcium Silicate-Based Cements. Physicochemical and Biological Properties

In vitro biocompatibility of nanostructured endodontic materials using SCAP cells

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