Addition: Column Characterization and Selection Systems in Reversed-Phase High-Performance Liquid Chromatography

Optimation of Combination of N-Hexane Solution and Ethyle Acetate on Secondary Metabolite Compounds Profile of Streptomyces hygroscopicus

“…Fractionation II uses a flow rate of 5 mL/minute while fractionation I uses a flow rate of 10 mL/minute. This difference will significantly affect the interaction time between the stationary phase of the column and the compounds contained in the extract so that it may produce chromatogram profiles with different retention times (18).…”

“…The pore size will affect the column area where the smaller the pore size between particles will increase the area that can interact with compound molecules. However, if the pore size is too small, it can reduce the efficiency and resolution of large molecules (18).…”

PoreMS: a software tool for generating silica pore models with user-defined surface functionalisation and pore dimensions

“…Chromatographic silica is known for its disordered pore structure and wide pore size distribution. Average pore diameters of 9-10 nm are fairly standard for RPLC columns intended for small molecules [22,23].…”

“…Molecular simulations, particularly atomistic molecular dynamics (MD) simulations, have already been used to elucidate the mass transport characteristics of different liquid chromatography modes [10][11][12][13][14][15][16] and recently to model catalytic [17][18][19] and drug delivery systems [20]. This suggests that molecular simulations are increasing in importance as an essential building block in multiscale modelling of heterogeneous catalysis [21] and the selection and development of chromatographic columns [22][23][24][25]. The molecular insight gained by such studies was used, for example, to obtain a unified description of diffusion inside mesoporous and microporous structures [26], to rationalise the effect of surface chemistry on olefin metathesis in confined geometries [17], and to provide fundamental insight into reactive ionic liquid films for supported ionic liquid phase (SILP) catalysis relevant for electrochemical systems or the water-gas shift reaction [27][28][29].…”

Сорбенты Для ВЭЖХ. Современное Состояние И Новые Направления Развития (Обзор)

“…Силикагели со средними размерами пор 80, 100, 120 Å применяют для разделения низкомолекулярных соединений, а силикагели с порами 300 Å -для разделения высокомолекулярных соединений и биополимеров. Приводим перечень обзоров по сорбентам для ВЭЖХ [2][3][4][5][6][7][8][9] [111,110] показано, что они могут успешно использоваться и в ВЭЖХ для экспрессных разделений. На жидких кристаллах хорошо разделяются изомеры [111,112].…”