Adding exogenous protein relieves the toxicity of nanoparticles to anammox granular sludge by adsorption and the formation of eco-coronas

Ma, Wenjie; Wang, Xin; Zhang, Jiangtao; Guo, Jie-Yun; Lin, Yan-Xu; Yao, Yu-Xi; Li, Guifeng; Cheng, Ya-Fei; Fan, Nian-Si; Jin, Ren‐Cun

doi:10.1039/d1en01138c

Cited by 4 publications

(5 citation statements)

References 60 publications

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“…During the incubation period, a greater number of BP aggregates were observed on the bottom of the EPEC incubation system compared to B. tropicus (Figure S10a). Previous studies have demonstrated adsorption of extracellular polymeric substances on the surface of nanoparticles, effectively altering aggregation processes in aqueous environments. , This process will prevent direct contact between the nanoparticles and cell membrane, thereby decreasing cytotoxicity . Given this, we hypothesize that BP nanosheets promoted protein production and secretion in EPEC, effectively mediating toxicity alleviation.…”

Section: Resultsmentioning

confidence: 82%

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Transcriptome Analysis Reveals the Growth Promotion Mechanism of Enteropathogenic Escherichia coli Induced by Black Phosphorus Nanosheets

et al. 2023

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With the extensive production and application of black phosphorus (BP) nanosheets, release to the environment is inevitable, which raises concerns about the fate and effects of this two-dimensional (2D) material on sensitive receptors such as environmental microbes. Although the bacterial toxicity of BP nanosheets has been demonstrated, whether the biological response differs in pathogenic and nonpathogenic strains of a microorganism is unknown. Here, enteropathogenic Escherichia coli (EPEC) and nonpathogenic Escherichia coli DH5α (E. coli DH5α), Escherichia coli k12 (E. coli k12), and Bacillus tropicus (B. tropicus) are used to comparatively study the microbial toxicity of BP nanosheets. Upon exposure to BP nanosheets across a range of doses from 10 to 100 μg mL −1 for 12 h, EPEC experienced enhanced growth and E. coli DH5α and E. coli k12 were not affected, whereas B. tropicus exhibited clear toxicity. By combining transcriptome sequencing, proteome analysis, and other sensitive biological techniques, the mechanism of BPinduced growth promotion for EPEC was uncovered. Briefly, BP nanosheets activate the antioxidation system to resist oxidative stress, promote protein synthesis and secretion to attenuate membrane damage, enhance the energy supply, and activate growth-related pathways. None of these impacts were evident with nonpathogenic strains. By describing the mechanism of strain-dependent microbial effects, this study not only highlights the potential risks of BP nanosheets to the environment and to human health but also calls attention to the importance of model strain selection when evaluating the hazard and toxicity of emerging nanomaterials.

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Section: Resultsmentioning

confidence: 82%

“…62,63 This process will prevent direct contact between the nanoparticles and cell membrane, 64 thereby decreasing cytotoxicity. 65 Given this, we hypothesize that BP nanosheets promoted protein production and secretion in EPEC, effectively mediating toxicity alleviation.…”

mentioning

confidence: 98%

Transcriptome Analysis Reveals the Growth Promotion Mechanism of Enteropathogenic Escherichia coli Induced by Black Phosphorus Nanosheets

et al. 2023

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“…Interestingly, natural organic matter was shown to have strong adsorption affinity to metal NPs, and the coating on the NP surface modulated eco-corona formation through ligand exchange and encapsulation mechanism attributed to surface functional groups. The biomolecules encapsulated on the surface of NPs also contributed to eco-corona formation through enhanced aggregation and negative charge properties while altering NP-induced biological processes . These findings implied that the role of eco-corona formation by proteins, lipids, and other natural metabolites in the bio–nano (or biomolecular–nano) system cannot be ignored.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The biomolecules encapsulated on the surface of NPs also contributed to eco-corona formation through enhanced aggregation and negative charge proper-ties 34 while altering NP-induced biological processes. 56 These findings implied that the role of eco-corona formation by proteins, 29 lipids, and other natural metabolites 57 in the bio− nano (or biomolecular−nano) system 58 cannot be ignored.…”

Section: Evolution Of the Active Interface Between Nps And Rootsmentioning

confidence: 99%

Driving Role of Zinc Oxide Nanoparticles with Different Sizes and Hydrophobicity in Metabolic Response and Eco-Corona Formation in Sprouts (Vigna radiata)

Kang,

Bai,

Liu

et al. 2024

Environ. Sci. Technol.

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Zinc oxide nanoparticles (ZnO NPs) cause biotoxicity and pose a potential ecological threat; however, their effects on plant metabolism and eco-corona evolution between NPs and organisms remain unclear. This study clarified the molecular mechanisms underlying physiological and metabolic responses induced by three different ZnO NPs with different sizes and hydrophobicity in sprouts (Vigna radiata) and explored the critical regulation of eco-corona formation in root–nano systems. Results indicated that smaller-sized ZnO inhibited root elongation by up to 37.14% and triggered oxidative burst and apoptosis. Metabolomics confirmed that physiological maintenance after n-ZnO exposure was mainly attributed to the effective stabilization of nitrogen fixation and defense systems by biotransformation of the flavonoid pathway. Larger-sized or hydrophobic group-modified ZnO exhibited low toxicity in sprouts, with 0.89-fold upregulation of citrate in central carbon metabolism. This contributed to providing energy for resistance to NP stress through amino acid and carbon/nitrogen metabolism, accompanied by changes in membrane properties. Notably, smaller-sized and hydrophobic NPs intensely stimulated the release of root metabolites, forming corona complexes with exudates. The hydrogen-bonded wrapping mechanism in protein secondary structure and hydrophobic interactions of heterogeneous functional groups drove eco-corona formation, along with the corona evolution intensity of n-ZnO > s-ZnO > b-ZnO based on higher (α-helix + 3-turn helix)/β-sheet ratios. This study provides crucial insight into metabolic and eco-corona evolution in bionano fates.

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“…Commercial γ-Fe 2 O 3 NPs (99.5% purity, 20 nm) were purchased from Sinopharm, China. Briefly, the mixture of γ-Fe 2 O 3 NPs and sodium dodecylbenzenesulfonate with a ratio of 1:0.017 (w/w) was homogenized in an ultrasonic cell disruptor (Ningbo Scientz Biotechnology, China) at 40 kHz and 250 W. 15 The γ-Fe 2 O 3 NP suspension was prepared on a daily basis to avoid aggregation.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Insight into the Regulation Mechanism of Iron Oxide Nanoparticles in Anammox Consortia: Autophagy-Dependent Ferroptosis

Wang,

Hou,

Wang

et al. 2024

ACS EST Water

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Iron oxide nanoparticles (IONPs) have been widely used and pose a high risk to human and animal health. In this study, the fate and regulation mechanism of γ-Fe2O3 NPs in an anaerobic ammonium oxidation (anammox) system were studied from the perspective of morphology, biotransformation, and microbial interaction. The lowest nitrogen removal efficiency (NRE) of the anammox process was 63.8% under γ-Fe2O3 NP stress. The Fe(II) and Fe(III) concentrations increased with the bioaccumulation of γ-Fe2O3 NPs, which caused high-level reactive oxygen species (ROS) and ferroptosis in the anammox consortia. They inhibited the synthesis pathways of ATP and heme c, which further reduced the detoxification ability of microbiota. Moreover, Fe(II) could be oxidized to Fe(III) in the form of Fe(III)-O, which formed biocrusts on the cell surface and limited the microbial substrate utilization. Microbial community analysis showed that the low-concentration γ-Fe2O3 NPs increased the abundance of functional bacteria related to nitrogen transformation, while 50 mg L–1 of γ-Fe2O3 NPs significantly inhibited their activity and metabolism. These findings deepen our understanding of the Fe–N network and provide a guidance for the practical application and operation of anammox process, especially in treating wastewater containing iron oxide nanomaterials.

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Adding exogenous protein relieves the toxicity of nanoparticles to anammox granular sludge by adsorption and the formation of eco-coronas

Abstract: MgO nanoparticles (NPs) are widely used in bactericides, fuel cells and electronic materials and are frequently detected in aquatic environments. Anaerobic ammonium oxidation (anammox) is a highly efficient and economical...

Cited by 4 publications

References 60 publications

Transcriptome Analysis Reveals the Growth Promotion Mechanism of Enteropathogenic Escherichia coli Induced by Black Phosphorus Nanosheets

Transcriptome Analysis Reveals the Growth Promotion Mechanism of Enteropathogenic Escherichia coli Induced by Black Phosphorus Nanosheets

Driving Role of Zinc Oxide Nanoparticles with Different Sizes and Hydrophobicity in Metabolic Response and Eco-Corona Formation in Sprouts (Vigna radiata)

Insight into the Regulation Mechanism of Iron Oxide Nanoparticles in Anammox Consortia: Autophagy-Dependent Ferroptosis

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