Addi-Chek filtration, BACTEC, and 10-ml culture methods for recovery of microorganisms from dialysis effluent during episodes of peritonitis

Males, B M; Walshe, J. Joseph; Garringer, L; Koscinski, D; Amsterdam, Daniel

doi:10.1128/jcm.23.2.350-353.1986

Cited by 29 publications

(29 citation statements)

References 8 publications

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“…Several studies have shown that culture rates increased significantly with the BACTEC blood culture system compared with conventional culture systems [3,5,9,12]. In our study, we also found that the BACTEC blood culture system increased the culture positivity (73/127) compared with direct inoculation of the centrifuged deposit of fluid into different culture media (63/127).…”

supporting

confidence: 74%

“…The low culture positivity in patients with peritonitis on CAPD has been attributed to the low load of the organisms in the majority of the patients, the presence of an antimicrobial agent in the peritoneal fluid of the patient receiving antimicrobial therapy, the presence of intracellular organisms, peritonitis caused by endotoxin released by bacterial infection and the culture technique used for the isolation of the organism [9]. Different investigators have used several methods to enhance the yield of organisms from the CAPD effluent, including the use of mass spectroscopy and sequencing and MALDI-TOF [3][4][5][6][7][8][9][10][11]. In the present study, we have observed high culture positivity with water lysis specimens and specimens pretreated with Triton-X and Tween-80 incorporated blood agar when compared with the automated blood culture system and direct inoculation of the centrifuged deposit of the specimen into different culture media.…”

mentioning

confidence: 99%

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Evaluation of different culture methods for the diagnosis of peritonitis in patients on continuous ambulatory peritoneal dialysis

Iyer

Reddy²,

Gande

et al. 2014

Clinical Microbiology and Infection

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A total of 170 continuous ambulatory peritoneal dialysis (CAPD) fluids were processed by various culture methods, including direct inoculation of the centrifuged sediment, inoculation into automated blood culture bottles, water lysis, Tween-80 incorporated blood agar, and Triton-X treatment of the specimen. Of 170 CAPD fluids, 127 showed the growth of bacteria/fungi. Sixty-three fluids showed growth by all methods, the water lysis alone detected 24 additional positive cultures, while Tween-80 blood agar and Triton-X yielded 30 additional positive cultures. A combination of water lysis, Tween-80 blood agar and Triton-X treatment of the CAPD fluid is recommended for diagnosis of CAPD peritonitis in resource-limited settings.

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supporting

confidence: 74%

mentioning

confidence: 99%

Evaluation of different culture methods for the diagnosis of peritonitis in patients on continuous ambulatory peritoneal dialysis

Iyer

Reddy²,

Gande

et al. 2014

Clinical Microbiology and Infection

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“…Generally, the larger the volume of effluent cultured, the higher the detection rates. However, comparative studies indicate that the minimum volume that should be tested is 10 ml (5,13,15,21). The method chosen should include an enrichment broth with antiphagocytic and lytic properties (3,18,19).…”

mentioning

confidence: 99%

“…The method chosen should include an enrichment broth with antiphagocytic and lytic properties (3,18,19). Both centrifugation (12,13,20) and filtration (11)(12)(13)(14)20) have been tested as means of concentrating bacteria from CAPD effluents. Semiautomated blood culture systems have been used for the detection of peritonitis in CAPD effluents.…”

mentioning

confidence: 99%

Improved detection of bacterial growth in continuous ambulatory peritoneal dialysis effluent by use of BacT/Alert FAN bottles

et al. 1997

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Culture-negative peritonitis is a major complication for patients on continuous ambulatory peritoneal dialysis (CAPD) and precludes organism-specific therapy. The aim of the present study was to compare inoculation of 10 ml of CAPD effluent into BacT/Alert blood culture bottles (FAN [fastidious antimicrobic neutralizing], BacTAlert aerobic [BTA], and BacT/Alert anaerobic [BTAn] bottles) to our conventional method of using 50 ml of concentrated CAPD effluent to inoculate peptone broth bottles (BD bottles) and MacConkey agar and blood agar medium (BA-MAC). The FAN, BTA, and BTAn bottles were monitored automatically in the BacT/Alert blood culture instrument. A total of 207 CAPD effluents were studied, and in 97 bacteria were detected by at least one method. Compared to BTA bottles (79 of 97; 81.4%), BTAn bottles (78 of 97; 80.4%), and BD bottles (88 of 97; 90.7%), the single best broth medium for detecting bacterial growth in CAPD effluents was the FAN bottle (90 of 97 effluents; 92.8%). A total of 125 bacterial species were detected by any method, and the majority (91.8%) of CAPD effluents were infected with a single species. A combination of FAN and BTAn bottles detected 111 of 125 (88.8%) of all organisms, whereas a combination of BD bottles and BA-MAC detected 107 of 125 (85.6%) of all organisms. One or more organisms that would have been completely missed by the

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“…Numerous studies have documented the advantage of using blood-culture bottle kits to improve the microbial yield of peritoneal fluid culture [2,[4][5][6][7][8][9]. The widely used BacT/AlerT In this study, we evaluated the performance of inoculation and culture of PD effluent using FAN Plus blood culture media.…”

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confidence: 99%

Evaluation of BacT/Alert FAN Plus Bottles for the Culture of Peritoneal Dialysate

et al. 2019

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Background: A major complication of peritoneal dialysis (PD) is peritonitis, and bacterial culture of PD effluent in a blood culture bottle is the preferred technique for diagnosis of peritonitis. In this study, we compared dialysate inoculation and culture using the BacT/AlerT Ⓡ Fastidious Antimicrobial Neutralization Plus blood culture bottles (FAN Plus; bioMérieux, France) to the conventional centrifugation culture method. Methods: A total of 170 PD effluents were simultaneously processed by the conventional centrifugation culture method and by culture using FAN Plus media with two different inoculation procedures: inoculation after centrifugation and direct bedside inoculation. Results: Of the 52 cultures that were positive on at least one of the culture methods, 27 samples were positive on conventional centrifugation. However, 46 samples showed growth following inoculation into the FAN Plus media after centrifugation, and 47 samples were positive on the direct FAN Plus inoculation method. Using the case definition for PD peritonitis to classify samples, sensitivity of the conventional method was 50.0% (95% CI, 33.7-66.3%), whereas the sensitivity of the FAN Plus media was 78.9% (95% CI, 62.2-89.9%) by inoculation after centrifugation and 86.8% (95% CI, 71.1-95.1%) by direct inoculation. Use of both inoculation methods with FAN Plus media resulted in 92.1% sensitivity (95% CI, 89.2-99.9%). Conclusion: Culture using FAN Plus media demonstrated a superior bacterial recovery rate to the conventional centrifugation culture method. A combination of the two inoculation methods with FAN Plus media is recommended for the best diagnostic yield, while direct inoculation alone can be useful due to its simplicity and cost-effectiveness. (Ann Clin Microbiol 2019;22:90-95)

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Addi-Chek filtration, BACTEC, and 10-ml culture methods for recovery of microorganisms from dialysis effluent during episodes of peritonitis

Cited by 29 publications

References 8 publications

Evaluation of different culture methods for the diagnosis of peritonitis in patients on continuous ambulatory peritoneal dialysis

Evaluation of different culture methods for the diagnosis of peritonitis in patients on continuous ambulatory peritoneal dialysis

Improved detection of bacterial growth in continuous ambulatory peritoneal dialysis effluent by use of BacT/Alert FAN bottles

Evaluation of BacT/Alert FAN Plus Bottles for the Culture of Peritoneal Dialysate

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