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Beckerle, Mary C.; Crawford, Aaron W.; Bertagnolli, Maria E.; Locke, Sarah J.; Miller, Dale

doi:10.1002/ar.1092270316

Cited by 2 publications

(4 citation statements)

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“…As measured in vitro, each of the proteins in a focal adhesion has multiple, interdependent interactions, the nature of which may change as each is assembled into a functional structure (Niggli and Gimona, 1993). For exam-talin is seen to accumulate at the plasma membrane (Beckerle et al, 1989). The recruitment of talin to such membrane sites in vivo may involve binding to integrin.…”

Section: Introductionmentioning

confidence: 99%

Talin requires beta-integrin, but not vinculin, for its assembly into focal adhesion-like structures in the nematode Caenorhabditis elegans.

Moulder

Huang

Waterston

et al. 1996

MBoC

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In cultured cells, the 230-kDa protein talin is found at discrete plasma membrane foci known as focal adhesions, sites that anchor the intracellular actin cytoskeleton to the extracellular matrix. The regulated assembly of focal adhesions influences the direction of cell migrations or the reorientation of cell shapes. Biochemical studies of talin have shown that it binds to the proteins integrin, vinculin, and actin in vitro. To understand the function of talin in vivo and to correlate its in vitro and in vivo biochemical properties, various genetic approaches have been adopted. With the intention of using genetics in the study of talin, we identified a homologue to mouse talin in a genetic model system, the nematode Caenorhabditis elegans. C. elegans talin is 39% identical and 59% similar to mouse talin. In wild-type adult C. elegans, talin colocalizes with integrin, vinculin, and alpha-actinin in the focal adhesion-like structures found in the body-wall muscle. By examining the organization of talin in two different C. elegans mutant strains that do not make either beta-integrin or vinculin, we were able to determine that talin does not require vinculin for its initial organization at the membrane, but that it depends critically on the presence of integrin for its initial assembly at membrane foci.

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Section: Introductionmentioning

confidence: 99%

Talin requires beta-integrin, but not vinculin, for its assembly into focal adhesion-like structures in the nematode Caenorhabditis elegans.

Moulder

Huang

Waterston

et al. 1996

MBoC

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“…In BS-C-1 cells, a tumor promoter-induced increase in talin phosphorylation was correlated with a dramatic loss in the integrity of focal contacts and organized actin filament arrays (Turner et al, 1989). In chicken embryo fibroblasts, a similar increase in talin phosphorylation did not result in a detectable loss of established adhesion plaques or stress fibers; however, in this case, the tumor promoter did induce a loss of talin-rich focal contact precursors (Beckerle, 1990).…”

Section: Talinmentioning

confidence: 72%

“…It is an in vitro substrate for purified protein kinase C at serine and threonine residues (Litchfield and Ball, 1986). When chicken embryo fibroblasts were exposed to the tumorpromoting phorbol ester, 12-myristate 13 acetate, which augments protein kinase C activity, phosphorylation on serine and threonine residues of talin increased three fold (Beckerle, 1990). The phosphorylation sites are located mainly on the 47 kDa talin fragment (Beckerle, 1990).…”

Section: Talinmentioning

confidence: 99%

“…When chicken embryo fibroblasts were exposed to the tumorpromoting phorbol ester, 12-myristate 13 acetate, which augments protein kinase C activity, phosphorylation on serine and threonine residues of talin increased three fold (Beckerle, 1990). The phosphorylation sites are located mainly on the 47 kDa talin fragment (Beckerle, 1990). A recent study showed that the 47 kDa fragment of talin also is a substrate for protein kinase P, which is a protein kinase that exhibits phospholipid-stimulation in the presence of Mn^^ (Simons and Elias, 1993).…”

Section: Talinmentioning

confidence: 99%

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The interaction between actin filaments and the cytoskeleton-membrane attachment site protein talin

Zhang

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Addenda

Abstract: Actin-membrane interaction at sites of cellsubstratum adhesion.

Cited by 2 publications

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Talin requires beta-integrin, but not vinculin, for its assembly into focal adhesion-like structures in the nematode Caenorhabditis elegans.

Talin requires beta-integrin, but not vinculin, for its assembly into focal adhesion-like structures in the nematode Caenorhabditis elegans.

The interaction between actin filaments and the cytoskeleton-membrane attachment site protein talin

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