Added Value of Biophysics to Study Lipid-Driven Biological Processes: The Case of Surfactins, a Class of Natural Amphiphile Molecules

Gilliard, Guillaume; Furlan, Aurélien L.; Smeralda, Willy; Pršić, Jelena; Deleu, Magali

doi:10.3390/ijms232213831

Cited by 7 publications

(2 citation statements)

References 80 publications

(247 reference statements)

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“…Consequently, we concluded that the DMPC/SF complex spontaneously assembles into nanodiscs in aqueous solutions. The interaction between SF and biomimetic membrane models has been previously reported: atomic-force microscopy imaging on lipid monolayers confirmed the formation of SF-rich clusters upon mixing SF with DMPC [ 25 , 26 ]. This phase separation phenomenon is associated with the strong interactions between SF molecules.…”

Section: Resultsmentioning

confidence: 90%

Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation

Imura,

Yanagisawa,

Ikeda

et al. 2024

Molecules

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Nanodiscs belong to a category of water-soluble lipid bilayer nanoparticles. In vivo nanodisc platforms are useful for studying isolated membrane proteins in their native lipid environment. Thus, the development of a practical method for nanodisc reconstruction has garnered consider-able research interest. This paper reports the self-assembly of a mixture of bio-derived cyclic peptide, surfactin (SF), and l-α-dimyristoylphosphatidylcholine (DMPC). We found that SF induced the solubilization of DMPC multilamellar vesicles to form their nanodiscs, which was confirmed by size-exclusion chromatography, dynamic light scattering, and transmission electron microscopy analyses. Owing to its amphiphilic nature, the self-assembled structure prevents the exposure of the hydrophobic lipid core to aqueous media, thus embedding ubiquinol (CoQ10) as a hydrophobic model compound within the inner region of the nanodiscs. These results highlight the feasibility of preparing nanodiscs without the need for laborious procedures, thereby showcasing their potential to serve as promising carriers for membrane proteins and various organic compounds. Additionally, the regulated self-assembly of the DMPC/SF mixture led to the formation of fibrous architectures. These results show the potential of this mixture to function as a nanoscale membrane surface for investigating molecular recognition events.

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Section: Resultsmentioning

confidence: 90%

Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation

Imura,

Yanagisawa,

Ikeda

et al. 2024

Molecules

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“…Lateral membrane heterogeneities could also play a crucial role. For the Bacillus CLiP surfactin, for example, it was shown that it inserts preferentially at the boundaries between the gel and liquid phases of model membranes (Deleu et al, 2013;Gilliard et al, 2022). However, effects of sterols may be much more specific than on order and thickness only.…”

Section: Implications For Antimicrobial Activitymentioning

confidence: 99%

The effect of membrane thickness on the membrane permeabilizing activity of the cyclic lipopeptide tolaasin II

Steigenberger

Mergen²,

Roo³

et al. 2022

Front. Mol. Biosci.

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Tolaasin II is an amphiphilic, membrane-active, cyclic lipopeptide produced by Pseudomonas tolaasii and is responsible for brown blotch disease in mushroom. To better understand the mode of action and membrane selectivity of tolaasin II and related lipopeptides, its permeabilizing effect on liposomes of different membrane thickness was characterized. An equi-activity analysis served to distinguish between the effects of membrane partitioning and the intrinsic activity of the membrane-bound peptide. It was found that thicker membranes require higher local peptide concentrations to become leaky. More specifically, the mole ratio of membrane-bound peptide per lipid needed to induce 50% leakage of calcein within 1 h, Re50, increased monotonically with membrane thickness from 0.0016 for the 14:1 to 0.0070 for the 20:1 lipid-chains. Moreover, fast but limited leakage kinetics in the low-lipid regime were observed implying a mode of action based on membrane asymmetry stress in this time and concentration window. While the assembly of the peptide to oligomeric pores of defined length along the bilayer z-axis can in principle explain inhibition by increasing membrane thickness, it cannot account for the observed limited leakage. Therefore, reduced intrinsic membrane-permeabilizing activity with increasing membrane thickness is attributed here to the increased mechanical strength and order of thicker membranes.

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Deciphering the distinct biocontrol activities of lipopeptides fengycin and surfactin through their differential impact on lipid membranes

Gilliard,

Demortier,

Boubsi

et al. 2024

Colloids and Surfaces B: Biointerfaces

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Added Value of Biophysics to Study Lipid-Driven Biological Processes: The Case of Surfactins, a Class of Natural Amphiphile Molecules

Cited by 7 publications

References 80 publications

Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation

Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation

The effect of membrane thickness on the membrane permeabilizing activity of the cyclic lipopeptide tolaasin II

Deciphering the distinct biocontrol activities of lipopeptides fengycin and surfactin through their differential impact on lipid membranes

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