Added Clinical Value of Whole-mount Histopathology of Radical Prostatectomy Specimens: A Collaborative Review

Cimadamore, Alessia; Liu, Cheng; López-Beltrán, Antonio; Mazzucchelli, Roberta; Lucianò, Roberta; Scarpelli, Marina; Montorsi, Francesco; Montironi, Rodolfo

doi:10.1016/j.euo.2020.08.003

Cited by 15 publications

(17 citation statements)

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“…To the best of our knowledge, no prior studies have reported on the systemic dehydration of liver tissue specimens > 10 cm in size ( 14 , 17 , 19 ). Dehydration for large liver tissue pathological investigations should endeavor to prevent wrinkling, maintain tissue hardness, and facilitate sectioning.…”

Section: Discussionmentioning

confidence: 99%

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Characteristic of Perineural Invasion in Hilar Cholangiocarcinoma Based on Whole-Mount Histologic Large Sections of Liver

et al. 2022

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Background & ObjectivePerineural invasion is an important biological feature of hilar cholangiocarcinoma (HCCA). We developed a whole-mount histologic large sections (WHLS) of the liver to evaluate peripheral nerve invasion (PNI) of HCCA.MethodsUsing sampling, fixation, dehydration, embedding, sectioning, hematoxylin and eosin (H&E) and immunohistochemical (IHC) staining, and scanning, the characteristics of intrahepatic and extrahepatic PNI in 20 patients with Bismuth type III and type IV HCCA were analyzed with WHLS. Correlation between the characteristics of nerve invasion and tumor size, vascular invasion (artery, portal vein), degree of differentiation, microvascular invasion (MVI), carbohydrate antigen19-9 (CA19-9), and differentiation degree of HCCA was statistically evaluated.ResultsThe WHLS of the liver was successfully established, which enabled us to observe intrahepatic and extrahepatic distribution of HCCA and whether surrounding tissues including nervous, blood, and lymph vessels were infiltrated. Extrahepatic and intrahepatic PNI were identified in 20 (100%) patients and 1 (5.0%) patient, respectively. Vessel density decreased in most invaded nerves presented by CD-34, which correlated with 100% of poorly differentiated and 83% of moderately differentiated tumors (P<0.008).ConclusionThis study established a WHLS of the liver that can be used for clinical diagnosis and research, and confirmed that extrahepatic PNI is prevalent, but intrahepatic nerve invasion is rare and does not accompany the invasion scope of bile ducts in types III and IV HCCA. In addition, moderately and poorly differentiated malignant tumors are more prone to PNI, independent of blood supply.

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Section: Discussionmentioning

confidence: 99%

“…WHLS technology was first introduced in 1994 for the diagnosis and staging of breast, prostate, endometrial, and lung cancers as well as brain tumors (15,(17)(18)(19). However, no systematic studies on hepatic WHLS larger than 5cm have been reported.…”

Section: Introductionmentioning

confidence: 99%

Characteristic of Perineural Invasion in Hilar Cholangiocarcinoma Based on Whole-Mount Histologic Large Sections of Liver

et al. 2022

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“…Importantly, however, it has been shown in other tissue types, where whole-mount histopathology is more commonly obtained, that examining the whole cassette does not appear to be superior in detecting anomalous pathological features. 31 Future directions of this work include a study with a larger sample size; more complete evaluation of the surgical specimen, including comparing examination of the entire surgical specimen and a single representative slice; and an in-depth analysis of exactly how MRI acquisition parameters and differences in hardware interact with the values of texture analysis features of VS. In addition, a study focused on the differences between previously irradiated and nonirradiated tumors may be of interest as the underlying mechanisms that lead to necrosis differ between these two contexts.…”

Section: Discussionmentioning

confidence: 99%

“…Concern might naturally arise for undersampling and the underdetection of hemosiderin, mucin, lymphocytes, and necrosis in the tumor and for a single section's potential failure to accurately represent the tumor's overall cellularity. Importantly, however, it has been shown in other tissue types, where whole‐mount histopathology is more commonly obtained, that examining the whole cassette does not appear to be superior in detecting anomalous pathological features 31 …”

Section: Discussionmentioning

confidence: 99%

MRI Texture Features are Associated with Vestibular Schwannoma Histology

et al. 2020

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Objectives/Hypothesis: To determine if commonly used radiomics features have an association with histological findings in vestibular schwannomas (VS).Study Design: Retrospective case-series. Methods: Patients were selected from an internal database of those who had a gadolinium-enhanced T1-weighted MRI scan captured prior to surgical resection of VS. Texture features from the presurgical magnetic resonance image (MRI) were extracted, and pathologists examined the resected tumors to assess for the presence of mucin, lymphocytes, necrosis, and hemosiderin and used a validated computational tool to determine cellularity. Sensitivity, specificity, and positive likelihood ratios were also computed for selected features using the Youden index to determine the optimal cut-off value.Results: A total of 45 patients were included. We found significant associations between multiple MRI texture features and the presence of mucin, lymphocytes, hemosiderin, and cellularity. No significant associations between MRI texture features and necrosis were identified. We were able to identify significant positive likelihood ratios using Youden index cut-off values for mucin (2.3; 95% CI 1.2-4.3), hemosiderin (1.5; 95% CI 1.04-2.1), lymphocytes (3.8; 95% CI 1.2-11.7), and necrosis (1.5; 95% CI 1.1-2.2).Conclusions: MRI texture features are associated with underlying histology in VS.

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“…In the case of radical prostatectomies, the significance of these practical limitations should not be underestimated: a survey by Samaratunga et al found that only 16% of 148 respondents reported on using WMS for their radical prostatectomy sectioning. 3 Despite the benefits of WMS, particularly in radical prostatectomy, 2 it is evident that their acquisition may not be feasible for every centre due to the technical limitations regarding whole-slide scanner availability and specimen size. In these cases, the benefits of WMS may still be obtainable if the individual tissue fragments could be "stitched" together into an artificial WMS, resembling the original specimen cross-section.…”

Section: Purposementioning

confidence: 99%

PythoStitcher: an iterative approach for stitching digitized tissue fragments into full resolution whole-mount reconstructions

Schouten

Litjens

2023

Medical Imaging 2023: Digital and Computational Pathology

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In histopathology, whole-mount sections (WMS) can be utilized to capture entire cross-sections of excised tissue, thereby reducing the number of slides per case, preserving tissue context, and minimizing cutting artefacts. Additionally, the use of WMS allows for easier correlation of histology and pre-operative imaging. However, in digital pathology, WMS are not frequently acquired due to the limited availability of whole-slide scanners that can scan double-width glass slides. Moreover, whole-mounts may still be too large to fit on a single double-width glass slide, and archival material is typically stored as tissue fragments. In this work, we present PythoStitcher, an improved version of the AutoStitcher algorithm for automatically stitching multiple tissue fragments and constructing a full-resolution WMS. PythoStitcher is based on a genetic algorithm that iteratively optimizes the affine transformation matrix for each tissue fragment using the Euclidean distance between corner points of neighbouring fragments. We demonstrate the efficiency and generalisability of PythoStitcher in a pilot validation of ten prostatectomy cases with four fragments and two oesophagectomy cases with two fragments. On average, PythoStitcher obtained the final stitching result in 60 seconds (range 51-73) for the prostatectomy cases and 68 seconds (range 43-94) for the oesophagectomy cases. The stitching accuracy was objectively evaluated by computing the average Euclidean distance between all corner points from the edges of neighbouring fragments. For the reconstructed WMS at full resolution, the average residual registration error was 1.6 mm (range 0.5-3.2) for the prostatectomy cases and 2.1 mm (range 0.5-3.8) for the oesophagectomy cases. The key strengths of PythoStitcher include its computational efficiency, the ability to reconstruct the WMS in full resolution, and an open-source implementation in Python.

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Added Clinical Value of Whole-mount Histopathology of Radical Prostatectomy Specimens: A Collaborative Review

Cited by 15 publications

References 50 publications

Characteristic of Perineural Invasion in Hilar Cholangiocarcinoma Based on Whole-Mount Histologic Large Sections of Liver

Characteristic of Perineural Invasion in Hilar Cholangiocarcinoma Based on Whole-Mount Histologic Large Sections of Liver

MRI Texture Features are Associated with Vestibular Schwannoma Histology

PythoStitcher: an iterative approach for stitching digitized tissue fragments into full resolution whole-mount reconstructions

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