Add salt, add sugar: N-glycosylation in <i>Haloferax volcanii</i>

Kaminski, Lina; Naparstek, Shai; Kandiba, Lina; Cohen-Rosenzweig, Chen; Arbiv, Adi; Konrad, Zvia; Eichler, Jerry

doi:10.1042/bst20120142

Cited by 14 publications

(11 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is unclear if pili or other types of lectin molecules are involved in cellular interactions that initiate Haloferax fusion events. Similar to our findings, different Haloferax species are also known to be differentially glycosylated (45) leading to semi-specific cell-cell recognition (44). Cell fusion between different Haloferax species could also be observed but with far lower efficiency (46).…”

Section: Discussionsupporting

confidence: 90%

Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns

Wolferen

Shajahan

Heinrich

et al. 2019

Preprint

View full text Add to dashboard Cite

18The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-19 specific cellular aggregates. Within these aggregates, cells exchange DNA in order to repair 20 DNA double strand breaks via homologous recombination. Substitution of the S. 21 acidocaldarius pilin subunits UpsA and UpsB with their homologs from Sulfolobus tokodaii 22 42 43 glycan chain (Glc 1 Man 2 GlcNAc 2 QuiS, containing glucose, mannose, N-acetylglucosamine 132 and the Sulfolobus-specific sulfoquinovose residues) (37) (Figure 2). The addition of N-133 acetylgucosamine or glucose did not result in altered cellular aggregation (Figure 2A and B); 134 however, in the presence of mannose, cell aggregates were significantly smaller ( Figure 2B). 135This suggests that the mannose molecules partially saturate the binding sites of the Ups-pili 136 and thereby inhibit interactions between pili and the glycan chains on the S-layer of the host 137 cell resulting in reduced aggregation. 138Defining the glycosylation pattern of S. tokodaii S-layer proteins 139

show abstract

Section: Discussionsupporting

confidence: 90%

Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns

Wolferen

Shajahan

Heinrich

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Interestingly, the modification of S-layer proteins seems to be of a highly dynamic nature in Archaea and can be modified reversibly in response to altering environmental conditions (84). Glycosylation seems to have an adaptive role, enabling the alteration of cell surface properties (e.g., charge distribution, hydration layer, or interaction between cell surface proteins) to provide protection, promote survival against rapidly changing environmental conditions, and establish inter-or intraspecies interactions (82,85). Moreover, the large S-layer subunit conserved in all Thaumarchaeota clusters in two different COGs representing the two orders Ca.…”

Section: Evidence For Exopolysaccharide Production and Cell Surfacementioning

confidence: 99%

Proteomics and comparative genomics of Nitrososphaera viennensis reveal the core genome and adaptations of archaeal ammonia oxidizers

Kerou

Offre

Valledor

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

166

196

View full text Add to dashboard Cite

Ammonia-oxidizing archaea (AOA) are among the most abundant microorganisms and key players in the global nitrogen and carbon cycles. They share a common energy metabolism but represent a heterogeneous group with respect to their environmental distribution and adaptions, growth requirements, and genome contents. We report here the genome and proteome of Nitrososphaera viennensis EN76, the type species of the archaeal class Nitrososphaeria of the phylum Thaumarchaeota encompassing all known AOA. N. viennensis is a soil organism with a 2.52-Mb genome and 3,123 predicted proteincoding genes. Proteomic analysis revealed that nearly 50% of the predicted genes were translated under standard laboratory growth conditions. Comparison with genomes of closely related species of the predominantly terrestrial Nitrososphaerales as well as the more streamlined marine Nitrosopumilales [Candidatus (Ca.) order] and the acidophile "Ca. Nitrosotalea devanaterra" revealed a core genome of AOA comprising 860 genes, which allowed for the reconstruction of central metabolic pathways common to all known AOA and expressed in the N. viennensis and "Ca. Nitrosopelagicus brevis" proteomes. Concomitantly, we were able to identify candidate proteins for as yet unidentified crucial steps in central metabolisms. In addition to unraveling aspects of core AOA metabolism, we identified specific metabolic innovations associated with the Nitrososphaerales mediating growth and survival in the soil milieu, including the capacity for biofilm formation, cell surface modifications and cell adhesion, and carbohydrate conversions as well as detoxification of aromatic compounds and drugs. ammonia oxidation | proteomics | archaea | comparative genomics | biofilm

show abstract

“…volcanii and S. acidocaldarius, the S-layer glycoprotein has been used extensively as the model reporter of N-glycosylation (6,107,118,222). This protein is particularly important in Archaea, as opposed to Bacteria, since most Archaea, including these two organisms (63,223), have a cell envelope consisting solely of an S-layer overlying a cytoplasmic membrane without the benefit of an intermediate peptidoglycan equivalent, a situation never found in S-layer-bearing Bacteria (224,225).…”

Section: Biological Effects Of N-linked Glycosylation Perturbations Imentioning

confidence: 99%

N-Linked Glycosylation in Archaea: a Structural, Functional, and Genetic Analysis

Jarrell

Ding

Meyer

et al. 2014

Microbiol Mol Biol Rev

Self Cite

180

214

View full text Add to dashboard Cite

SUMMARY N-glycosylation of proteins is one of the most prevalent posttranslational modifications in nature. Accordingly, a pathway with shared commonalities is found in all three domains of life. While excellent model systems have been developed for studying N-glycosylation in both Eukarya and Bacteria , an understanding of this process in Archaea was hampered until recently by a lack of effective molecular tools. However, within the last decade, impressive advances in the study of the archaeal version of this important pathway have been made for halophiles, methanogens, and thermoacidophiles, combining glycan structural information obtained by mass spectrometry with bioinformatic, genetic, biochemical, and enzymatic data. These studies reveal both features shared with the eukaryal and bacterial domains and novel archaeon-specific aspects. Unique features of N-glycosylation in Archaea include the presence of unusual dolichol lipid carriers, the use of a variety of linking sugars that connect the glycan to proteins, the presence of novel sugars as glycan constituents, the presence of two very different N-linked glycans attached to the same protein, and the ability to vary the N-glycan composition under different growth conditions. These advances are the focus of this review, with an emphasis on N-glycosylation pathways in Haloferax , Methanococcus , and Sulfolobus .

show abstract

Add salt, add sugar: N-glycosylation in Haloferax volcanii

Cited by 14 publications

References 31 publications

Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns

Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns

Proteomics and comparative genomics of Nitrososphaera viennensis reveal the core genome and adaptations of archaeal ammonia oxidizers

N-Linked Glycosylation in Archaea: a Structural, Functional, and Genetic Analysis

Contact Info

Product

Resources

About