Adar-mediated A-to-I editing is required for embryonic patterning and innate immune response regulation in zebrafish

Liang

et al. 2023

Preprint

The significance of A-to-I RNA editing in neurological development is widely recognized, however, its influence on retina development remains to be thoroughly understood. In this study, we aimed to characterize the temporal landscape of A-to-I editing in mouse retina development using total RNA-seq and Ribosome profiling, with a specific emphasis on its effect on gene translation. Our findings revealed that the editing underwent plastic changes and distinct editing contents facilitated stage-specific functions. Further analysis showed a dynamic interplay between A-to-I editing and alternative splicing, with A-to-I editing having a significant impact on splicing efficiency and increasing the quantity of splicing events. A-to-I editing held the potential of enhancing the translatome's diversity, but this came at the expense of reduced translational efficiency. When coupled with splicing, it could produce a coordinated regulatory effect on translatome dynamics. Overall, our study presents a temporally resolved atlas of A-to-I editing, connecting its dynamic changes with the regulatory impact on splicing and translation.

Section: Resultssupporting

confidence: 94%

“…Adar-mediated A-to-I editing has been established as crucial for normal development of organisms 33,43,44 . Disruptions to Adar can lead to serious consequences such as locomotion and neuron defects seen in flies with mutant Adar 45 .…”

Section: Discussionmentioning

confidence: 99%

Temporal landscape and translational regulation of A-to-I editing in mouse retina development

Liang

et al. 2023

Preprint

“…1 B; Additional file 1 : Supplementary Tables 2 and 3). This proportion is in line with prior research findings [ 25 , 26 ]. Among these A-to-I editing sites, 8104 were previously reported by REDIportal, while the remaining 7005 were newly discovered, and both exhibited a similar motif pattern (Additional file 2 : Fig.…”

Section: Resultssupporting

confidence: 93%

“…ADAR-mediated A-to-I editing has been established as crucial for the normal development of organisms [ 26 , 35 , 36 ]. Disruptions to ADAR can lead to serious consequences such as locomotion and neuron defects seen in flies with mutant ADAR [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Temporal landscape and translational regulation of A-to-I RNA editing in mouse retina development

Yang,

Yi,

Yang

et al. 2024

BMC Biol

Background The significance of A-to-I RNA editing in nervous system development is widely recognized; however, its influence on retina development remains to be thoroughly understood. Results In this study, we performed RNA sequencing and ribosome profiling experiments on developing mouse retinas to characterize the temporal landscape of A-to-I editing. Our findings revealed temporal changes in A-to-I editing, with distinct editing patterns observed across different developmental stages. Further analysis showed the interplay between A-to-I editing and alternative splicing, with A-to-I editing influencing splicing efficiency and the quantity of splicing events. A-to-I editing held the potential to enhance translation diversity, but this came at the expense of reduced translational efficiency. When coupled with splicing, it could produce a coordinated effect on gene translation. Conclusions Overall, this study presents a temporally resolved atlas of A-to-I editing, connecting its changes with the impact on alternative splicing and gene translation in retina development.

“…In mammals, there are two common forms of RNA editing: A-to-I and cytidine to uridine (C-to-U). The former is catalysed by a family of enzymes called adenosine deaminases acting on RNA (ADARs) ( Figure 2D ) ( Yang et al, 2021 ; Niescierowicz et al, 2022 ). Recent evidence indicates the capacity of A-to-I editing to modulate snoRNA precursors.…”

Section: Biogenesis and Function Of Snornasmentioning

confidence: 99%

Small but strong: the emerging role of small nucleolar RNA in cardiovascular diseases

Sun,

Wang,

Luo

et al. 2023

Front. Cell Dev. Biol.

Cardiovascular diseases (CVDs) are the leading cause of mortality and disability worldwide. Numerous studies have demonstrated that non-coding RNAs (ncRNAs) play a primary role in CVD development. Therefore, studies on the mechanisms of ncRNAs are essential for further efforts to prevent and treat CVDs. Small nucleolar RNAs (snoRNAs) are a novel species of non-conventional ncRNAs that guide post-transcriptional modifications and the subsequent maturation of small nuclear RNA and ribosomal RNA. Evidently, snoRNAs are extensively expressed in human tissues and may regulate different illnesses. Particularly, as the next-generation sequencing techniques have progressed, snoRNAs have been shown to be differentially expressed in CVDs, suggesting that they may play a role in the occurrence and progression of cardiac illnesses. However, the molecular processes and signaling pathways underlying the function of snoRNAs remain unidentified. Therefore, it is of great value to comprehensively investigate the association between snoRNAs and CVDs. The aim of this review was to collate existing literature on the biogenesis, characteristics, and potential regulatory mechanisms of snoRNAs. In particular, we present a scientific update on these snoRNAs and their relevance to CVDs in an effort to cast new light on the functions of snoRNAs in the clinical diagnosis of CVDs.