Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator

Wang, Peng; Siao, Wei; Zhao, Xiuyang; Arora, Deepanksha; Ren, Wei; Eeckhout, Dominique; Leene, Jelle Van; Kumar, Rahul; Houbaert, Anaxi; Winne, Nancy De; Mylle, Evelien; Vandorpe, Michaël; Korver, Ruud A.; Testerink, Christa; Gevaert, Kris; Vanneste, Steffen; Jaeger, Geert De; Damme, Daniël Van; Russinova, Eugenia

doi:10.1038/s41477-022-01328-2

Cited by 7 publications

(5 citation statements)

References 88 publications

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“…For the past decade, research on constructing large‐scale plant protein–protein interaction (PPI) networks has mainly focused on model plants, and the capability of experimental‐based large‐scale PPI networks in these plants was thought to be significantly inferior to the actual number of protein interactions in plants (Altmann et al ., 2020; Jones et al ., 2014; Smakowska‐Luzan et al ., 2018; Wang et al ., 2023). Currently, there is still a lack of experimentally‐derived, large‐scale PPI data in plants, especially with respect to protein interaction networks specific to seed organs (Rhee and Mutwil, 2014).…”

Section: Figurementioning

confidence: 99%

BnSTINet: An experimentally‐based transcription factor interaction network in seeds of Brassica napus

Yin,

Jia,

et al. 2024

Plant Biotechnology Journal

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Section: Figurementioning

confidence: 99%

BnSTINet: An experimentally‐based transcription factor interaction network in seeds of Brassica napus

Yin,

Jia,

et al. 2024

Plant Biotechnology Journal

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“…A conserved function in autophagy of AtEH proteins 11 , and Ede1p and Pan1p in yeast 21,22 support the idea that they are functional homologs. We previously showed that AtEH1 co-purifies with AP-2 9,14 , and that AtEH1 interacts with anionic phospholipids and cargo 12 . Interactomics and live cell imaging suggest TPC assembles as an octameric complex on the membrane during the initiation of endocytosis 9,23 , with the lipid binding proteins AtEH1, AtEH2, and the muniscin-like protein TML forming an interface with the plasma membrane 13 .…”

Section: Introductionmentioning

confidence: 99%

“…TPC is essential for plant life, as viable knock-out mutants in individual subunits have not been identified [9][10][11] . Recent studies have established the critical role of TPC during endocytosis by demonstrating its interaction with many essential endocytic components, including anionic phospholipids 12,13 , AP-2 9,14 , clathrin 15 , and ubiquitinated cargo 16 . Furthermore, destabilisation of TPC leads to impaired endocytic internalisation, and is correlated with a disruption in membrane bending during clathrin-coated pit formation 17,18 .…”

Section: Introductionmentioning

confidence: 99%

Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants

Damme

Dragwidge

Wang

et al. 2023

Preprint

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Clathrin-mediated endocytosis is an essential cellular internalisation pathway involving the dynamic assembly of clathrin and accessory proteins to form membrane-bound vesicles. The evolutionarily ancient TSET/TPLATE complex (TPC) plays an essential, but not well-defined role in endocytosis in plants. Here, we show that two highly disordered TPC subunits, AtEH1 and AtEH2 function as scaffolds to drive biomolecular condensation of the complex. These condensates specifically nucleate on the plasma membrane through interactions with anionic phospholipids, and facilitate the dynamic recruitment and assembly of clathrin, early-, and late-stage endocytic accessory proteins. Importantly, clathrin forms ordered assemblies within the condensate environment. Biomolecular condensation therefore acts to promote dynamic protein assemblies throughout clathrin-mediated endocytosis. Furthermore, the disordered region sequence properties of AtEH1 regulate the material properties of the endocytic condensates in vivo. Alteration of the material properties influences endocytosis dynamics, and thereby impairs environmental adaption. In conclusion, our findings reveal how collective interactions shape endocytosis.

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“…TPC is essential for plant life, as viable knock-out mutants have not been identified (Gadeyne et al, 2014; Van Damme et al, 2006; Wang et al, 2019). Recent studies have established the critical role of TPC during endocytosis by demonstrating its interaction with many essential endocytic components, including anionic phospholipids (Yperman et al, 2021a; Yperman et al, 2021b), the AP-2 complex (Gadeyne et al, 2014; Wang et al, 2023), clathrin (Van Damme et al, 2011), and ubiquitinated cargo (Grones et al, 2022). Furthermore, destabilisation of TPC leads to impaired endocytic internalisation, and disrupts membrane bending during clathrin-coated pit formation (Johnson et al, 2021; Wang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…A conserved function in autophagy of AtEH proteins (Wang et al, 2019), and Ede1p and Pan1p in yeast (Liu et al, 2022; Wilfling et al, 2020) support the idea that they are functional homologs. We previously showed that AtEH1 co-purifies with AP-2 (Gadeyne et al, 2014; Wang et al, 2023), and AtEH1 interacts with anionic phospholipids and cargo (Yperman et al, 2021a). Interactomics and live cell imaging suggest TPC assembles as an octameric complex on the membrane during the initiation of endocytosis (Gadeyne et al, 2014; Wang et al, 2020), with the lipid binding proteins AtEH1, AtEH2, and the muniscin-like protein TML forming an interface with the plasma membrane (Yperman et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants

Dragwidge

Wang

Brocard³

et al. 2022

Preprint

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Endocytosis is the process by which cells internalise molecules from their cell surface via plasma membrane-derived vesicles. In plants, clathrin-mediated endocytosis requires the evolutionarily ancient TSET/TPLATE complex (TPC), which was lost in metazoan and fungal lineages. TPC is required for membrane bending, but how TPC functions in the initiation of endocytosis and clathrin assembly is unclear. Here we used live-cell imaging and biochemical approaches to investigate the function of the Arabidopsis thaliana TPC subunit AtEH1/Pan1. Using in vitro and in vivo experiments we found that AtEH/Pan1 proteins can self-assemble into condensates through phase separation, which is influenced by both structured and intrinsically disordered regions. The proteome composition of these condensates revealed many key endocytic components which are selectively recruited via prion-like- and IDR-based interactions, including the ESCRT-0 TOM-Like proteins. Furthermore, AtEH/Pan1 condensates selectively nucleate on the plasma membrane by binding specific phospholipid species that are recognised by their EH domains. Visualization of the ultrastructure of the endocytic condensates via CLEM-ET revealed that the coat protein clathrin can assemble into lattices within condensates. Our results reveal that AtEH/Pan1 proteins act as scaffolds to direct endocytic machinery to specific plasma membrane regions to initiate internalisation. These findings provide new insight into the interplay between membranes and protein condensates.

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Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator

Cited by 7 publications

References 88 publications

BnSTINet: An experimentally‐based transcription factor interaction network in seeds of Brassica napus

BnSTINet: An experimentally‐based transcription factor interaction network in seeds of Brassica napus

Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants

Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants

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